Restriction map for
pFN21AE6439
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CpoI DraII DraIII EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRV FauI FbaI FseI FspI
HgaI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 954 GCGG CCGC(-3/-1)
AlwNI 1360 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1190 AGCGCT AGC^GCT
AseI 1384 ATTAAT AT^TAAT
AsnI 1384 ATTAAT AT^TAAT
Asp700I 86 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1152 TGGCCA TGG^CCA
BanI 7 GGTGCC G^G[CT][GA]CC
BbrPI 1027 CACGTG CAC^GTG
BcnI 334 CCGGG CC^[GC]GG
BfaI 880 CTAG C^TAG
BglII 1259 AGATCT A^GATCT
BsaAI 1027 CACGTG [CT]AC^GT[GA]
BsmI 817 GAATGC GAATGC(1/-1)
BspDI 1388 ATCGAT AT^CGAT
BspHI 1121 TCATGA T^CATGA
BsrDI 636 CATTGC GCAATG(2/0)
BsrFI 223 GCCGGC [GA]^CCGG[CT]
BstXI 1022 CCATCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1191 GCGC GCG^C
Cfr10I 223 GCCGGC [GA]^CCGG[CT]
ClaI 1388 ATCGAT AT^CGAT
DraI 1264 TTTAAA TTT^AAA
DrdI 589 GACTTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1152 TGGCCA [CT]^GGCC[GA]
Eco47III 1190 AGCGCT AGC^GCT
EcoNI 193 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 751 GAATTC G^AATTC
EcoT14I 1105 CCATGG C^C[AT][AT]GG
EcoT22I 819 ATGCAT ATGCA^T
HaeII 1190 AGCGCT [GA]GCGC^[CT]
HhaI 1191 GCGC GCG^C
HinP1I 1191 GCGC G^CGC
HincII 1110 GTTAAC GT[CT]^[GA]AC
HindII 1110 GTTAAC GT[CT]^[GA]AC
HpaI 1110 GTTAAC GTT^AAC
MaeI 880 CTAG C^TAG
MaeII 1028 ACGT A^CGT
MluNI 1152 TGGCCA TGG^CCA
MscI 1152 TGGCCA TGG^CCA
MspA1I 1175 CAGCTG C[AC]G^C[GT]G
NaeI 223 GCCGGC GCC^GGC
NciI 334 CCGGG CC^[GC]GG
NcoI 1105 CCATGG C^CATGG
NgoMIV 223 GCCGGC G^CCGGC
NsiI 819 ATGCAT ATGCA^T
PmaCI 1027 CACGTG CAC^GTG
PmlI 1027 CACGTG CAC^GTG
PshBI 1384 ATTAAT AT^TAAT
PvuII 1175 CAGCTG CAG^CTG
RcaI 1121 TCATGA T^CATGA
StuI 854 AGGCCT AGG^CCT
StyI 1105 CCATGG C^C[AT][AT]GG
XcmI 307 CCAGTAGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 86 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 751 GAATTC [GA]^AATT[CT]
991 AAATTT
Alw44I 542 GTGCAC G^TGCAC
957 GTGCAC
AocI 103 CCTAAGG CC^T[ACGT]AGG
967 CCTGAGG
ApaLI 542 GTGCAC G^TGCAC
957 GTGCAC
ApoI 751 GAATTC [GA]^AATT[CT]
991 AAATTT
AspHI 542 GTGCAC G[AT]GC[AT]^C
957 GTGCAC
AvaII 36 GGTCC G^G[AT]CC
425 GGACC
BamHI 1219 GGATCC G^GATCC
1413 GGATCC
BbvI 1174 GCAGC GCAGC(8/12)
1177 GCTGC
BsiHKAI 542 GTGCAC G[AT]GC[AT]^C
957 GTGCAC
Bsu36I 103 CCTAAGG CC^T[ACGT]AGG
967 CCTGAGG
Cac8I 223 GCCGGC GC[ACGT]^[ACGT]GC
630 GCCAGC
DsaI 495 CCACGG C^C[GA][CT]GG
1105 CCATGG
Eco81I 103 CCTAAGG CC^T[ACGT]AGG
967 CCTGAGG
Fnu4HI 1174 GCAGC GC^[ACGT]GC
1177 GCTGC
ItaI 1174 GCAGC GC^[ACGT]GC
1177 GCTGC
MwoI 435 GCTCTCCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
812 GCCATGAATGC
ScaI 794 AGTACT AGT^ACT
1070 AGTACT
SfaNI 45 GATGC GCATC(5/9)
301 GATGC
SfcI 322 CTACAG C^T[GA][CT]AG
1058 CTATAG
TaqI 1329 TCGA T^CGA
1389 TCGA
TseI 1174 GCAGC G^C[AT]GC
1177 GCTGC
TthHB8I 1329 TCGA T^CGA
1389 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII FokI
HaeIII HapII HinfI HpaII HphI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
Tsp45I Tsp509I TspRI XhoII