Restriction map for
pFN21AE6439
(Circular) (Six-base) MAPSORT of: pFN21AE6439.seq Check: 1539 from: 1 to: 6133
pFN21AE6439 6133 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2464 4196 5580 5848 1632
Size: 832 1732 1384 268 1917
Fragments arranged by size:
1917 1732 1384 832 268
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 6133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3370 3876 5448 3370
Size: 506 1572 4055
Fragments arranged by size:
4055 1572 506
ApaLI G'TGCA_C
Cuts at: 2547 2962 4190 5346 2547
Size: 415 1228 1156 3334
Fragments arranged by size:
3334 1228 1156 415
ApoI r'AATT_y
Cuts at: 1493 2756 2996 3442 3668 3820 5929 1493
Size: 1263 240 446 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 446 240 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3390 4687 160
Size: 872 2358 1297 1606
Fragments arranged by size:
2358 1606 1297 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BamHI G'GATC_C
Cuts at: 3224 3418 3463 3830 3224
Size: 194 45 367 5527
Fragments arranged by size:
5527 367 194 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 3454 4909 618
Size: 325 217 627 225 1442 1455 1842
Fragments arranged by size:
1842 1455 1442 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 3452 1391
Size: 2061 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BfrBI ATG'CAT
Cuts at: 2826 2826
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3264 6128 3264
Size: 2864 3269
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2474 2551 2966 4194 5350 1790
Size: 684 77 415 1228 1156 2573
Fragments arranged by size:
2573 1228 1156 684 415 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2306 2616 3864
Size: 416 53 83 399 24 889 310 1248
Cuts at: 3864 4822 442
Size: 958 1753
Fragments arranged by size:
1753 1248 958 889 416 399 310 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4793 1239
Size: 3554 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3034 3924 493
Size: 711 1830 890 2702
Fragments arranged by size:
2702 1830 890 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4566 4964 5238 5385 5796 4566
Size: 398 274 147 411 4903
Fragments arranged by size:
4903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6133
BseYI C'CCAG_C
Cuts at: 2156 2284 5336 5832 2156
Size: 128 3052 496 2457
Fragments arranged by size:
3052 2457 496 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 2966 4194 4279 5350 2551
Size: 415 1228 85 1071 3334
Fragments arranged by size:
3334 1228 1071 415 85
BsmI GAATG_Cn'
Cuts at: 2828 3644 3737 2828
Size: 816 93 5224
Fragments arranged by size:
5224 816 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2474 2551 2966 3452 4194 4279 5350
Size: 399 684 77 415 486 742 85 1071
Cuts at: 5350 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 684 486 415 399 85
77
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 3126 4022 5752 3126
Size: 896 1730 3507
Fragments arranged by size:
3507 1730 896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3488 844
Size: 2644 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 4628 4802 65
Size: 1183 1392 1988 174 1396
Fragments arranged by size:
1988 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 4777 4964 1700
Size: 110 418 2549 187 2869
Fragments arranged by size:
2869 2549 418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstXI CCAn_nnnn'nTGG
Cuts at: 3034 3034
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2163 3224 3264 3418 3463 3830 4215 4232
Size: 191 1061 40 154 45 367 385 17
Cuts at: 4232 5684 5770 5782 6128 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1061 385 367 346 191 154
86 45 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2109 2973 2109
Size: 864 5269
BtgI C'CryG_G
Cuts at: 513 1065 2004 2500 3110 513
Size: 552 939 496 610 3536
Fragments arranged by size:
3536 939 610 552 496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2819 3649 4443 4463 2819
Size: 830 794 20 4489
Fragments arranged by size:
4489 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 3394 1554
Size: 1840 4293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3271 3437 3271
Size: 166 5967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 5140 809
Size: 507 1284 2540 1802
Fragments arranged by size:
2540 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 3580 4469
Size: 54 1425 50 275 18 1327 423 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1327 889 423 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2170 2213 2273 4063 1353
Size: 817 43 60 1790 3423
Fragments arranged by size:
3423 1790 817 60 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2464 4196 4793 5580 5848 1239
Size: 393 832 1732 597 787 268 1524
Fragments arranged by size:
1732 1524 832 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2202 2202
Size: 6133
EcoRI G'AATT_C
Cuts at: 2756 3442 3820 2756
Size: 686 378 5069
Fragments arranged by size:
5069 686 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2925 2925
Size: 6133
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 3199 5280 1678
Size: 1521 2081 2531
Fragments arranged by size:
2531 2081 1521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2496 2528 2717 2749 3012
Size: 32 135 32 924 32 189 32 263
Cuts at: 3012 3044 4774 4806 4848 4880 1373
Size: 32 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 924 263 189 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3477 3723 669
Size: 988 281 1179 360 246 3079
Fragments arranged by size:
3079 1179 988 360 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3117 3723 3117
Size: 606 5527
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 3409 5247 5431 1305
Size: 1455 649 1838 184 2007
Fragments arranged by size:
2007 1838 1455 649 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3159 10
Size: 54 1425 343 1327 2984
Fragments arranged by size:
2984 1425 1327 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 3032 3173 4091 4450 4609 518
Size: 648 1339 527 141 918 359 159 2042
Fragments arranged by size:
2042 1339 918 648 527 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 3182 3549 4226 5374 5619 1606
Size: 1576 367 677 1148 245 2120
Fragments arranged by size:
2120 1576 1148 677 367 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 6133
NcoI C'CATG_G
Cuts at: 513 1065 2004 3110 513
Size: 552 939 1106 3536
Fragments arranged by size:
3536 1106 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NsiI A_TGCA'T
Cuts at: 2828 2828
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PmlI CAC'GTG
Cuts at: 3034 3034
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3138 3703 3138
Size: 565 5568
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 3182 3182
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 2801 3077 4381 6093 2801
Size: 276 1304 1712 2841
Fragments arranged by size:
2841 1712 1304 276
SfcI C'TryA_G
Cuts at: 826 1045 2327 3063 3481 4616 5297 5488 826
Size: 219 1282 736 418 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 736 681 418 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2861 1517
Size: 1344 4789
StyI C'CwwG_G
Cuts at: 513 1065 2004 3110 3572 513
Size: 552 939 1106 462 3074
Fragments arranged by size:
3074 1106 939 552 462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 4160 4177 4330 4515 4960 325
Size: 1255 1719 861 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 861 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 3075 3094
Size: 274 80 33 51 1443 822 276 19
Cuts at: 3094 4379 6091 96
Size: 1285 1712 138
Fragments arranged by size:
1712 1443 1285 822 276 274 138 80
51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2352 2384 2637 2669 6093 6125 2352
Size: 32 253 32 3424 32 2360
Fragments arranged by size:
3424 2360 253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2319 2319
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 2095 4262 2095
Size: 2167 3966
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI