(Linear) (Six-base) MAPSORT of: KIEE6439.seq Check: 2177 from: 1 to: 1428 KIEE6439.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 459 1428 Size: 459 969 AfeI AGC'GCT Cuts at: 0 1192 1428 Size: 1192 236 AlwNI CAG_nnn'CTG Cuts at: 0 1365 1428 Size: 1365 63 ApaLI G'TGCA_C Cuts at: 0 542 957 1428 Size: 542 415 471 Fragments arranged by size: 542 471 415 ApoI r'AATT_y Cuts at: 0 751 991 1428 Size: 751 240 437 Fragments arranged by size: 751 437 240 AseI AT'TA_AT Cuts at: 0 1385 1428 Size: 1385 43 BamHI G'GATC_C Cuts at: 0 1219 1413 1428 Size: 1219 194 15 Fragments arranged by size: 1219 194 15 BanI G'GyrC_C Cuts at: 0 7 1428 Size: 7 1421 BfrBI ATG'CAT Cuts at: 0 821 1428 Size: 821 607 BglII A'GATC_T Cuts at: 0 1259 1428 Size: 1259 169 Bme1580I G_kGCm'C Cuts at: 0 469 546 961 1428 Size: 469 77 415 467 Fragments arranged by size: 469 467 415 77 BmrI ACTGGGnnnn_n' Cuts at: 0 301 611 1428 Size: 301 310 817 Fragments arranged by size: 817 310 301 BsaAI yAC'GTr Cuts at: 0 1029 1428 Size: 1029 399 BseYI C'CCAG_C Cuts at: 0 151 279 1428 Size: 151 128 1149 Fragments arranged by size: 1149 151 128 BsiHKAI G_wGCw'C Cuts at: 0 546 961 1428 Size: 546 415 467 Fragments arranged by size: 546 467 415 BsmI GAATG_Cn' Cuts at: 0 823 1428 Size: 823 605 Bsp1286I G_dGCh'C Cuts at: 0 469 546 961 1428 Size: 469 77 415 467 Fragments arranged by size: 469 467 415 77 BspHI T'CATG_A Cuts at: 0 1121 1428 Size: 1121 307 BsrDI GCAATG_nn' Cuts at: 0 635 1428 Size: 635 793 BsrFI r'CCGG_y Cuts at: 0 223 1428 Size: 223 1205 BstXI CCAn_nnnn'nTGG Cuts at: 0 1029 1428 Size: 1029 399 BstYI r'GATC_y Cuts at: 0 158 1219 1259 1413 1428 Size: 158 1061 40 154 15 Fragments arranged by size: 1061 158 154 40 15 Bsu36I CC'TnA_GG Cuts at: 0 104 968 1428 Size: 104 864 460 Fragments arranged by size: 864 460 104 BtgI C'CryG_G Cuts at: 0 495 1105 1428 Size: 495 610 323 Fragments arranged by size: 610 495 323 BtsI GCAGTG_nn' Cuts at: 0 814 1428 Size: 814 614 ClaI AT'CG_AT Cuts at: 0 1389 1428 Size: 1389 39 DraI TTT'AAA Cuts at: 0 1266 1428 Size: 1266 162 DrdI GACnn_nn'nnGTC Cuts at: 0 595 1428 Size: 595 833 EaeI y'GGCC_r Cuts at: 0 1152 1428 Size: 1152 276 EarI CTCTTCn'nnn_ Cuts at: 0 165 208 268 1428 Size: 165 43 60 1160 Fragments arranged by size: 1160 165 60 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 459 1428 Size: 459 969 EcoNI CCTnn'n_nnAGG Cuts at: 0 197 1428 Size: 197 1231 EcoRI G'AATT_C Cuts at: 0 751 1428 Size: 751 677 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 920 1428 Size: 920 508 HaeII r_GCGC'y Cuts at: 0 1194 1428 Size: 1194 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 491 523 712 744 1007 1039 1428 Size: 491 32 189 32 263 32 389 Fragments arranged by size: 491 389 263 189 32 32 32 HincII GTy'rAC Cuts at: 0 1112 1428 Size: 1112 316 HpaI GTT'AAC Cuts at: 0 1112 1428 Size: 1112 316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 755 1404 1428 Size: 755 649 24 Fragments arranged by size: 755 649 24 MscI TGG'CCA Cuts at: 0 1154 1428 Size: 1154 274 MslI CAynn'nnrTG Cuts at: 0 500 1027 1168 1428 Size: 500 527 141 260 Fragments arranged by size: 527 500 260 141 MspA1I CmG'CkG Cuts at: 0 1177 1428 Size: 1177 251 NaeI GCC'GGC Cuts at: 0 225 1428 Size: 225 1203 NcoI C'CATG_G Cuts at: 0 1105 1428 Size: 1105 323 NgoMIV G'CCGG_C Cuts at: 0 223 1428 Size: 223 1205 NsiI A_TGCA'T Cuts at: 0 823 1428 Size: 823 605 PmlI CAC'GTG Cuts at: 0 1029 1428 Size: 1029 399 PsiI TTA'TAA Cuts at: 0 1133 1428 Size: 1133 295 PvuII CAG'CTG Cuts at: 0 1177 1428 Size: 1177 251 ScaI AGT'ACT Cuts at: 0 796 1072 1428 Size: 796 276 356 Fragments arranged by size: 796 356 276 SfcI C'TryA_G Cuts at: 0 322 1058 1428 Size: 322 736 370 Fragments arranged by size: 736 370 322 StuI AGG'CCT Cuts at: 0 856 1428 Size: 856 572 StyI C'CwwG_G Cuts at: 0 1105 1428 Size: 1105 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1294 1428 Size: 1294 134 TatI w'GTAC_w Cuts at: 0 794 1070 1089 1428 Size: 794 276 19 339 Fragments arranged by size: 794 339 276 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 347 379 632 664 1428 Size: 347 32 253 32 764 Fragments arranged by size: 764 347 253 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 314 1428 Size: 314 1114 XmnI GAAnn'nnTTC Cuts at: 0 90 1428 Size: 90 1338 Enzymes that do cut: AcuI AfeI AlwNI ApaLI ApoI AseI BamHI BanI BfrBI BglII Bme1580I BmrI BsaAI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PmlI PsiI PvuII ScaI SfcI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI