Restriction map for
pFN21AB7579
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NlaIII NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 152 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 3 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 104 GGACC G^G[AT]CC
BamHI 263 GGATCC G^GATCC
BbvCI 124 GCTGAGG CCTCAGC(-5/-2)
BglII 633 AGATCT A^GATCT
BsaI 147 GGTCTC GGTCTC(1/5)
BsmAI 148 GTCTC GTCTC(1/5)
BsmFI 103 GGGAC GGGAC(10/14)
BsrBI 220 GAGCGG CCGCTC(-3/-3)
BsrFI 106 ACCGGC [GA]^CCGG[CT]
BsrI 568 CCAGT ACTGG(1/-1)
BstBI 365 TTCGAA TT^CGAA
BstNI 511 CCAGG CC^[AT]GG
Cfr10I 106 ACCGGC [GA]^CCGG[CT]
EarI 270 GAAGAG CTCTTC(1/4)
EcoNI 204 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 511 CCAGG ^CC[AT]GG
HinfI 409 GATTC G^A[ACGT]TC
Ksp632I 270 GAAGAG CTCTTC(1/4)
MvaI 511 CCAGG CC^[AT]GG
NdeI 330 CATATG CA^TATG
NspV 365 TTCGAA TT^CGAA
SapI 270 GAAGAGC GCTCTTC(1/4)
SfuI 365 TTCGAA TT^CGAA
SmlI 637 CTCAAG C^T[CT][GA]AG
TfiI 409 GATTC G^A[AT]TC
XmnI 3 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 264 GATCC GGATC(4/5)
263 GGATC
ApaI 160 GGGCCC GGGCC^C
183 GGGCCC
AvaI 165 CCCGGG C^[CT]CG[GA]G
187 CCCGGG
BanI 115 GGCACC G^G[CT][GA]CC
129 GGTGCC
BanII 160 GGGCCC G[GA]GC[CT]^C
183 GGGCCC
BbvI 70 GCTGC GCAGC(8/12)
200 GCTGC
BglI 49 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
85 GCCGCCTCGGC
BsoBI 165 CCCGGG C^[CT]CG[GA]G
187 CCCGGG
BstF5I 39 GGATG GGATG(2/0)
213 GGATG
BstYI 263 GGATCC [GA]^GATC[CT]
633 AGATCT
DraII 160 GGGCCCC [GA]G^G[ACGT]CC[CT]
183 GGGCCCC
DsaI 30 CCGCGG C^C[GA][CT]GG
74 CCGCGG
EcoO109I 160 GGGCCCC [GA]G^G[ACGT]CC[CT]
183 GGGCCCC
FokI 39 GGATG GGATG(9/13)
213 GGATG
KspI 30 CCGCGG CCGC^GG
74 CCGCGG
MflI 263 GGATCC [GA]^GATC[CT]
633 AGATCT
NotI 43 GCGGCCGC GC^GGCCGC
76 GCGGCCGC
SacII 30 CCGCGG CCGC^GG
74 CCGCGG
SmaI 165 CCCGGG CCC^GGG
187 CCCGGG
TaqI 299 TCGA T^CGA
366 TCGA
TseI 70 GCTGC G^C[AT]GC
200 GCTGC
Tsp509I 354 AATT ^AATT
455 AATT
TthHB8I 299 TCGA T^CGA
366 TCGA
XhoII 263 GGATCC [GA]^GATC[CT]
633 AGATCT
XmaCI 165 CCCGGG C^CCGGG
187 CCCGGG
XmaI 165 CCCGGG C^CCGGG
187 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI BcnI BmyI
BsaJI BsiEI BsiYI BslI Bsp1286I
BssKI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EagI EclXI
Eco52I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MnlI MseI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI SfaNI
Tru9I TspRI