Restriction map for
pFN21AB7579
(Linear) (Six-base) MAPSORT of: KIBB7579.seq Check: 9180 from: 1 to: 660
KIBB7579.orf
With 182 enzymes: SgfI *
July 3, 2012 16:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 660
Size: 237 423
AlwNI CAG_nnn'CTG
Cuts at: 0 157 660
Size: 157 503
ApaI G_GGCC'C
Cuts at: 0 164 187 660
Size: 164 23 473
Fragments arranged by size:
473 164 23
AvaI C'yCGr_G
Cuts at: 0 165 187 660
Size: 165 22 473
Fragments arranged by size:
473 165 22
BamHI G'GATC_C
Cuts at: 0 263 660
Size: 263 397
BanI G'GyrC_C
Cuts at: 0 115 129 660
Size: 115 14 531
Fragments arranged by size:
531 115 14
BanII G_rGCy'C
Cuts at: 0 164 187 660
Size: 164 23 473
Fragments arranged by size:
473 164 23
BbvCI CC'TCA_GC
Cuts at: 0 125 660
Size: 125 535
BglI GCCn_nnn'nGGC
Cuts at: 0 55 91 660
Size: 55 36 569
Fragments arranged by size:
569 55 36
BglII A'GATC_T
Cuts at: 0 633 660
Size: 633 27
Bme1580I G_kGCm'C
Cuts at: 0 118 164 187 660
Size: 118 46 23 473
Fragments arranged by size:
473 118 46 23
Bpu10I CC'TnA_GC
Cuts at: 0 125 660
Size: 125 535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 660
Size: 622 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 153 660
Size: 153 507
BseYI C'CCAG_C
Cuts at: 0 157 660
Size: 157 503
BsiEI CG_ry'CG
Cuts at: 0 47 80 176 231 660
Size: 47 33 96 55 429
Fragments arranged by size:
429 96 55 47 33
Bsp1286I G_dGCh'C
Cuts at: 0 118 164 187 660
Size: 118 46 23 473
Fragments arranged by size:
473 118 46 23
BsrBI CCG'CTC
Cuts at: 0 222 660
Size: 222 438
BsrFI r'CCGG_y
Cuts at: 0 106 660
Size: 106 554
BstBI TT'CG_AA
Cuts at: 0 366 660
Size: 366 294
BstYI r'GATC_y
Cuts at: 0 263 633 660
Size: 263 370 27
Fragments arranged by size:
370 263 27
BtgI C'CryG_G
Cuts at: 0 30 74 660
Size: 30 44 586
Fragments arranged by size:
586 44 30
BtsI GCAGTG_nn'
Cuts at: 0 59 68 660
Size: 59 9 592
Fragments arranged by size:
592 59 9
EaeI y'GGCC_r
Cuts at: 0 44 56 77 173 228 660
Size: 44 12 21 96 55 432
Fragments arranged by size:
432 96 55 44 21 12
EagI C'GGCC_G
Cuts at: 0 44 77 173 228 660
Size: 44 33 96 55 432
Fragments arranged by size:
432 96 55 44 33
EarI CTCTTCn'nnn_
Cuts at: 0 265 660
Size: 265 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 660
Size: 237 423
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 660
Size: 208 452
EcoO109I rG'GnC_Cy
Cuts at: 0 161 184 660
Size: 161 23 476
Fragments arranged by size:
476 161 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 547 660
Size: 547 113
MspA1I CmG'CkG
Cuts at: 0 32 76 154 660
Size: 32 44 78 506
Fragments arranged by size:
506 78 44 32
NdeI CA'TA_TG
Cuts at: 0 331 660
Size: 331 329
NotI GC'GGCC_GC
Cuts at: 0 44 77 660
Size: 44 33 583
Fragments arranged by size:
583 44 33
PspOMI G'GGCC_C
Cuts at: 0 160 183 660
Size: 160 23 477
Fragments arranged by size:
477 160 23
SacII CC_GC'GG
Cuts at: 0 33 77 660
Size: 33 44 583
Fragments arranged by size:
583 44 33
SapI GCTCTTCn'nnn_
Cuts at: 0 265 660
Size: 265 395
SmaI CCC'GGG
Cuts at: 0 167 189 660
Size: 167 22 471
Fragments arranged by size:
471 167 22
SmlI C'TyrA_G
Cuts at: 0 637 660
Size: 637 23
XmaI C'CCGG_G
Cuts at: 0 165 187 660
Size: 165 22 473
Fragments arranged by size:
473 165 22
XmnI GAAnn'nnTTC
Cuts at: 0 7 660
Size: 7 653
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BamHI BanI BanII BbvCI
BglI BglII Bme1580I Bpu10I BpuEI BsaI BseYI BsiEI
Bsp1286I BsrBI BsrFI BstBI BstYI BtgI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI MspA1I NdeI
NotI PspOMI SacII SapI SmaI SmlI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NgoMIV NheI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI ZraI