(Circular) (Six-base) MAPSORT of: pFN21AE2157.seq Check: 7587 from: 1 to: 5695 pFN21AE2157 5695 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3758 5142 5410 1632 Size: 654 1472 1384 268 1917 Fragments arranged by size: 1917 1472 1384 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2753 2951 4594 5571 1203 Size: 1550 198 1643 977 1327 Fragments arranged by size: 1643 1550 1327 977 198 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2248 1166 Size: 1082 4613 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 3438 5010 3438 Size: 1572 4123 ApaI G_GGCC'C Cuts at: 2634 2634 Size: 5695 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 2097 2894 3004 3230 3382 5491 1493 Size: 604 797 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 604 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2811 3020 1870 Size: 73 868 209 4545 Fragments arranged by size: 4545 868 209 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2957 2990 2957 Size: 33 5662 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3016 4471 618 Size: 325 217 627 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 627 325 217 BanII G_rGCy'C Cuts at: 1391 2540 2634 3014 1391 Size: 1149 94 380 4072 Fragments arranged by size: 4072 1149 380 94 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2348 2985 3587 4797 2348 Size: 637 602 1210 3246 Fragments arranged by size: 3246 1210 637 602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4306 136 Size: 107 122 71 631 605 2634 1525 Fragments arranged by size: 2634 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2634 3756 4912 1790 Size: 844 1122 1156 2573 Fragments arranged by size: 2573 1156 1122 844 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2673 2990 4355 1239 Size: 1434 317 1365 2579 Fragments arranged by size: 2579 1434 1365 317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 3820 4685 4983 2753 Size: 1067 865 298 3465 Fragments arranged by size: 3465 1067 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 4898 5394 4898 Size: 496 5199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3756 3841 4912 3756 Size: 85 1071 4539 Fragments arranged by size: 4539 1071 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2540 2634 3014 3756 3841 4912 1391 Size: 399 750 94 380 742 85 1071 2174 Fragments arranged by size: 2174 1071 750 742 399 380 94 85 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 3050 844 Size: 1571 635 3489 Fragments arranged by size: 3489 1571 635 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2939 4190 4364 65 Size: 1183 1691 1251 174 1396 Fragments arranged by size: 1691 1396 1251 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4339 4526 1700 Size: 110 2529 187 2869 Fragments arranged by size: 2869 2529 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2217 794 Size: 1423 4272 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2235 1184 Size: 1051 4644 BstYI r'GATC_y Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690 Size: 1053 367 385 17 1452 86 12 346 Cuts at: 5690 1972 Size: 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2092 513 Size: 552 1027 4116 Fragments arranged by size: 4116 1027 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 3211 4005 4025 3211 Size: 794 20 4881 Fragments arranged by size: 4881 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2577 2999 2577 Size: 422 5273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3142 4031 5660 Size: 54 1425 50 275 18 1312 889 1629 Cuts at: 5660 8 Size: 43 Fragments arranged by size: 1629 1425 1312 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2442 4282 4656 4802 387 Size: 1064 138 853 1840 374 146 1280 Fragments arranged by size: 1840 1280 1064 853 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2673 2990 3758 4355 5142 5410 Size: 393 654 387 317 768 597 787 268 Cuts at: 5410 1239 Size: 1524 Fragments arranged by size: 1524 787 768 654 597 393 387 317 268 EcoO109I rG'GnC_Cy Cuts at: 2631 2631 Size: 5695 EcoRI G'AATT_C Cuts at: 2097 3004 3382 2097 Size: 907 378 4410 Fragments arranged by size: 4410 907 378 EcoRV GAT'ATC Cuts at: 2043 2915 2043 Size: 872 4823 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 4842 1678 Size: 3164 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 4336 4368 4410 Size: 32 135 32 913 32 1819 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 1819 913 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 2864 748 Size: 2116 3579 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3002 4809 4993 1305 Size: 1697 1807 184 2007 Fragments arranged by size: 2007 1807 1697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2248 2602 2785 2799 2845 3653 4012 Size: 648 1082 354 183 14 46 808 359 Cuts at: 4012 4171 518 Size: 159 2042 Fragments arranged by size: 2042 1082 808 648 359 354 183 159 46 14 MspA1I CmG'CkG Cuts at: 1606 2891 3111 3788 4936 5181 1606 Size: 1285 220 677 1148 245 2120 Fragments arranged by size: 2120 1285 1148 677 245 220 NcoI C'CATG_G Cuts at: 513 1065 2092 513 Size: 552 1027 4116 Fragments arranged by size: 4116 1027 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2220 2757 2955 3053 4598 1132 Size: 97 991 537 198 98 1545 2229 Fragments arranged by size: 2229 1545 991 537 198 98 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 2753 2951 4594 2753 Size: 198 1643 3854 Fragments arranged by size: 3854 1643 198 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2770 1613 Size: 1157 4538 PsiI TTA'TAA Cuts at: 2832 3265 2832 Size: 433 5262 PspOMI G'GGCC_C Cuts at: 2630 2630 Size: 5695 PstI C_TGCA'G Cuts at: 830 3047 830 Size: 2217 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2891 2891 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2142 3043 4178 4859 5050 826 Size: 219 1097 901 1135 681 191 1471 Fragments arranged by size: 1471 1135 1097 901 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2732 3799 4700 4962 820 Size: 197 926 789 1067 901 262 1553 Fragments arranged by size: 1553 1067 926 901 789 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5695 StyI C'CwwG_G Cuts at: 513 1065 2092 3134 513 Size: 552 1027 1042 3074 Fragments arranged by size: 3074 1042 1027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3941 5653 Size: 274 80 33 51 1443 484 1480 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1480 1443 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI