Restriction map for
pFN21AE4504
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BcgI
BciVI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflII 811 CTTAAG C^TTAAG
AlwNI 1376 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1012 AGCGCT AGC^GCT
BamHI 963 GGATCC G^GATCC
BbrPI 1396 CACGTG CAC^GTG
BbsI 1459 GTCTTC GAAGAC(2/6)
BfrI 811 CTTAAG C^TTAAG
BlpI 372 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 372 GCTCAGC GC^T[ACGT]AGC
BpuAI 1459 GTCTTC GAAGAC(2/6)
BsaI 920 GAGACC GGTCTC(1/5)
BsmAI 920 GAGAC GTCTC(1/5)
BspHI 1529 TCATGA T^CATGA
BspLU11I 144 ACATGT A^CATGT
BsrDI 307 GCAATG GCAATG(2/0)
CelII 372 GCTCAGC GC^T[ACGT]AGC
CfoI 1013 GCGC GCG^C
DraI 528 TTTAAA TTT^AAA
DraII 1056 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1012 AGCGCT AGC^GCT
EcoO109I 1056 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1105 GATATC GAT^ATC
FauI 99 GCGGG CCCGC(4/6)
HaeII 1012 AGCGCT [GA]GCGC^[CT]
HgaI 7 GACGC GACGC(5/10)
HhaI 1013 GCGC GCG^C
HinP1I 1013 GCGC G^CGC
NcoI 256 CCATGG C^CATGG
PmaCI 1396 CACGTG CAC^GTG
PmlI 1396 CACGTG CAC^GTG
RcaI 1529 TCATGA T^CATGA
SapI 671 GCTCTTC GCTCTTC(1/4)
SbfI 51 CCTGCAGG CCTGCA^GG
SphI 1313 GCATGC GCATG^C
Sse8387I 51 CCTGCAGG CCTGCA^GG
StuI 740 AGGCCT AGG^CCT
Tsp45I 418 GTCAC ^GT[GC]AC
XbaI 267 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 144 ACATGT A^C[GA][CT]GT
1397 ACGTGT
Asp700I 738 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1455 GAAAGTCTTC
BalI 1007 TGGCCA TGG^CCA
1049 TGGCCA
BbvCI 448 GCTGAGG CCTCAGC(-5/-2)
901 GCTGAGG
BclI 299 TGATCA T^GATCA
558 TGATCA
BfaI 268 CTAG C^TAG
391 CTAG
BsaAI 874 TACGTG [CT]AC^GT[GA]
1396 CACGTG
BsgI 31 CTGCAC GTGCAG(16/14)
599 CTGCAC
BsmFI 5 GGGAC GGGAC(10/14)
1305 GTCCC
Bsp1407I 398 TGTACA T^GTACA
909 TGTACA
BspMI 50 ACCTGC ACCTGC(4/8)
462 GCAGGT
BsrBI 97 GAGCGG CCGCTC(-3/-3)
1209 GAGCGG
BsrGI 398 TGTACA T^GTACA
909 TGTACA
BsrI 116 ACTGG ACTGG(1/-1)
1005 ACTGG
BstAPI 471 GCACCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1375 GCAGGCTCTGC
BstYI 963 GGATCC [GA]^GATC[CT]
1026 AGATCC
EaeI 1007 TGGCCA [CT]^GGCC[GA]
1049 TGGCCA
EcoRI 930 GAATTC G^AATTC
1068 GAATTC
FbaI 299 TGATCA T^GATCA
558 TGATCA
MaeI 268 CTAG C^TAG
391 CTAG
MflI 963 GGATCC [GA]^GATC[CT]
1026 AGATCC
MluNI 1007 TGGCCA TGG^CCA
1049 TGGCCA
MscI 1007 TGGCCA TGG^CCA
1049 TGGCCA
SacI 669 GAGCTC GAGCT^C
1438 GAGCTC
SspBI 398 TGTACA T^GTACA
909 TGTACA
TaqI 1301 TCGA T^CGA
1463 TCGA
TfiI 430 GATTC G^A[AT]TC
633 GAATC
TthHB8I 1301 TCGA T^CGA
1463 TCGA
XhoII 963 GGATCC [GA]^GATC[CT]
1026 AGATCC
XmnI 738 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1455 GAAAGTCTTC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
Tru9I TseI Tsp509I TspRI