(Circular) (Six-base) MAPSORT of: pFN21AE4504.seq Check: 193 from: 1 to: 6289 pFN21AE4504 6289 bp With 182 enzymes: SgfI * March 28, 2012 14:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2458 2458 Size: 6289 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3632 1937 Size: 1695 4594 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2105 2117 2664 2759 2951 4352 5736 Size: 414 59 12 547 95 192 1401 1384 Cuts at: 5736 6004 1632 Size: 268 1917 Fragments arranged by size: 1917 1401 1384 547 414 268 192 95 59 12 AfeI AGC'GCT Cuts at: 3019 3019 Size: 6289 AflII C'TTAA_G Cuts at: 820 1017 2816 820 Size: 197 1799 4293 Fragments arranged by size: 4293 1799 197 AflIII A'CryG_T Cuts at: 1203 2149 3402 5188 6165 1203 Size: 946 1253 1786 977 1327 Fragments arranged by size: 1786 1327 1253 977 946 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2348 3168 1184 Size: 1164 820 4305 Fragments arranged by size: 4305 1164 820 AlwNI CAG_nnn'CTG Cuts at: 3386 4032 5604 3386 Size: 646 1572 4071 Fragments arranged by size: 4071 1572 646 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 2115 2935 3073 3492 3598 3824 3976 6085 Size: 622 820 138 419 106 226 152 2109 Cuts at: 6085 1493 Size: 1697 Fragments arranged by size: 2109 1697 820 622 419 226 152 138 106 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 3614 1870 Size: 73 1671 4545 Fragments arranged by size: 4545 1671 73 BamHI G'GATC_C Cuts at: 2968 3619 3986 2968 Size: 651 367 5271 Fragments arranged by size: 5271 651 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3610 5065 618 Size: 325 217 627 1823 1455 1842 Fragments arranged by size: 1842 1823 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2678 3447 3551 3608 1391 Size: 1287 769 104 57 4072 Fragments arranged by size: 4072 1287 769 104 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3457 928 Size: 971 1558 3760 Fragments arranged by size: 3760 1558 971 BbvCI CC'TCA_GC Cuts at: 2454 2907 2454 Size: 453 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4480 4514 1903 Size: 34 2543 34 3678 Fragments arranged by size: 3678 2543 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 2304 2563 1548 Size: 756 259 5274 Fragments arranged by size: 5274 756 259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4900 136 Size: 107 122 71 631 605 3228 1525 Fragments arranged by size: 3228 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 2378 3706 2378 Size: 1328 4961 Bme1580I G_kGCm'C Cuts at: 1790 2169 2337 3237 4350 5506 1790 Size: 379 168 900 1113 1156 2573 Fragments arranged by size: 2573 1156 1113 900 379 168 BmgBI CAC'GTC Cuts at: 1318 3184 1318 Size: 1866 4423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2131 4020 4978 Size: 416 53 83 399 24 714 1889 958 Cuts at: 4978 442 Size: 1753 Fragments arranged by size: 1889 1753 958 714 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2469 2943 4949 1239 Size: 1100 130 474 2006 2579 Fragments arranged by size: 2579 2006 1100 474 130 Bpu10I CC'TnA_GC Cuts at: 2454 2907 2454 Size: 453 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3431 4414 5279 5577 2333 Size: 1098 983 865 298 3045 Fragments arranged by size: 3045 1098 983 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2919 4952 882 Size: 617 1420 2033 2219 Fragments arranged by size: 2219 2033 1420 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 3403 4080 493 Size: 711 1677 522 677 2702 Fragments arranged by size: 2702 1677 711 677 522 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4096 4478 5113 275 Size: 53 83 186 936 2563 382 635 1451 Fragments arranged by size: 2563 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4722 5120 5394 5541 5952 4722 Size: 398 274 147 411 5059 Fragments arranged by size: 5059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 2442 2472 1178 Size: 30 165 30 993 30 16 30 4995 Fragments arranged by size: 4995 993 165 30 30 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2404 2702 3101 3315 3455 3515 1186 Size: 1218 298 399 214 140 60 3960 Fragments arranged by size: 3960 1218 399 298 214 140 60 BseYI C'CCAG_C Cuts at: 2018 3544 5492 5988 2018 Size: 1526 1948 496 2319 Fragments arranged by size: 2319 1948 1526 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2021 2589 1163 Size: 573 285 568 4863 Fragments arranged by size: 4863 573 568 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3739 4500 5528 1540 Size: 275 186 1738 761 1028 2301 Fragments arranged by size: 2301 1738 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 2678 3402 3447 4350 4435 5506 2478 Size: 200 724 45 903 85 1071 3261 Fragments arranged by size: 3261 1071 903 724 200 85 45 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2337 2478 2678 3237 3402 3447 Size: 399 379 168 141 200 559 165 45 Cuts at: 3447 3551 3608 4350 4435 5506 1391 Size: 104 57 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 379 200 168 165 141 104 85 57 45 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 3534 4178 5908 3534 Size: 644 1730 3915 Fragments arranged by size: 3915 1730 644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 2458 3644 844 Size: 1220 394 1186 3489 Fragments arranged by size: 3489 1220 1186 394 BsrBI CCG'CTC Cuts at: 1394 1823 2104 3216 4176 1394 Size: 429 281 1112 960 3507 Fragments arranged by size: 3507 1112 960 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 4784 4958 65 Size: 1183 1071 2465 174 1396 Fragments arranged by size: 2465 1396 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4933 5120 1700 Size: 110 3123 187 2869 Fragments arranged by size: 3123 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2403 2914 96 Size: 2307 511 3471 Fragments arranged by size: 3471 2307 511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 4349 5361 4349 Size: 1012 5277 BstAPI GCAn_nnn'nTGC Cuts at: 794 2482 3386 794 Size: 1688 904 3697 Fragments arranged by size: 3697 1688 904 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6289 BstYI r'GATC_y Cuts at: 1972 2968 3031 3619 3986 4371 4388 5840 5926 Size: 996 63 588 367 385 17 1452 86 Cuts at: 5926 5938 6284 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 996 588 385 367 346 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2261 2418 3200 513 Size: 552 939 132 125 157 782 3602 Fragments arranged by size: 3602 939 782 552 157 132 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3493 487 Size: 683 56 2267 3283 Fragments arranged by size: 3283 2267 683 56 BtsI GCAGTG_nn' Cuts at: 3805 4599 4619 3805 Size: 794 20 5475 Fragments arranged by size: 5475 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 2535 3593 2535 Size: 1058 5231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3012 3054 3736 Size: 54 1425 50 275 18 1182 42 682 Cuts at: 3736 4625 6254 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1182 889 682 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3736 1537 Size: 275 1924 4090 Fragments arranged by size: 4090 1924 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2070 2520 2679 2683 4219 1353 Size: 669 48 450 159 4 1536 3423 Fragments arranged by size: 3423 1536 669 450 159 48 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4876 5250 5396 387 Size: 1064 138 3287 374 146 1280 Fragments arranged by size: 3287 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2676 3445 2676 Size: 769 5520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2105 2117 2339 2469 2664 2759 Size: 393 414 59 12 222 130 195 95 Cuts at: 2759 2943 2951 4352 4949 5736 6004 1239 Size: 184 8 1401 597 787 268 1524 Fragments arranged by size: 1524 1401 787 597 414 393 268 222 195 184 130 95 59 12 8 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2891 3378 2060 Size: 831 487 4971 Fragments arranged by size: 4971 831 487 EcoO109I rG'GnC_Cy Cuts at: 3062 3062 Size: 6289 EcoRI G'AATT_C Cuts at: 2935 3073 3598 3976 2935 Size: 138 525 378 5248 Fragments arranged by size: 5248 525 378 138 EcoRV GAT'ATC Cuts at: 3112 3112 Size: 6289 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 3338 3481 2764 Size: 574 143 5572 Fragments arranged by size: 5572 574 143 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 3021 5436 1678 Size: 1343 2415 2531 Fragments arranged by size: 2531 2415 1343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373 Size: 32 135 32 3358 32 42 32 2626 Fragments arranged by size: 3358 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5403 5587 1305 Size: 4098 184 2007 Fragments arranged by size: 4098 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3014 3056 10 Size: 54 1425 343 1182 42 3243 Fragments arranged by size: 3243 1425 1182 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2330 3134 3150 4247 4606 4765 518 Size: 648 1164 804 16 1097 359 159 2042 Fragments arranged by size: 2042 1164 1097 804 648 359 159 16 MspA1I CmG'CkG Cuts at: 1606 3705 4382 5530 5775 1606 Size: 2099 677 1148 245 2120 Fragments arranged by size: 2120 2099 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2261 513 Size: 552 939 257 4541 Fragments arranged by size: 4541 939 552 257 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 2153 2804 3322 3647 5192 1132 Size: 97 924 651 518 325 1545 2229 Fragments arranged by size: 2229 1545 924 651 518 325 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6289 PciI A'CATG_T Cuts at: 2149 5188 2149 Size: 3039 3250 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6289 PfoI T'CCnGG_A Cuts at: 2650 2920 2976 2650 Size: 270 56 5963 Fragments arranged by size: 5963 270 56 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PmlI CAC'GTG Cuts at: 3403 3403 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6289 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PstI C_TGCA'G Cuts at: 830 2061 2514 3252 3641 830 Size: 1231 453 738 389 3478 Fragments arranged by size: 3478 1231 738 453 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 SacI G_AGCT'C Cuts at: 2678 3447 2678 Size: 769 5520 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SapI GCTCTTCn'nnn_ Cuts at: 2683 2683 Size: 6289 SbfI CC_TGCA'GG Cuts at: 2061 3641 2061 Size: 1580 4709 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SfcI C'TryA_G Cuts at: 826 1045 2057 2510 3248 3336 3637 4772 5453 Size: 219 1012 453 738 88 301 1135 681 Cuts at: 5453 5644 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1012 738 681 453 301 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3616 1872 Size: 1744 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 2816 3446 4393 5294 5556 Size: 197 926 405 468 630 947 901 262 Cuts at: 5556 820 Size: 1553 Fragments arranged by size: 1553 947 926 901 630 468 405 262 197 SnaBI TAC'GTA Cuts at: 493 4080 493 Size: 3587 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3322 3647 1132 Size: 2190 325 3774 Fragments arranged by size: 3774 2190 325 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 2747 1517 Size: 1230 5059 StyI C'CwwG_G Cuts at: 513 1065 2004 2261 2337 3237 3313 3728 513 Size: 552 939 257 76 900 76 415 3074 Fragments arranged by size: 3074 939 900 552 415 257 76 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3162 4316 4333 4486 4671 5116 325 Size: 1255 1582 1154 17 153 185 445 1498 Fragments arranged by size: 1582 1498 1255 1154 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 2403 2914 Size: 274 80 33 51 1443 411 15 511 Cuts at: 2914 4535 6247 96 Size: 1621 1712 138 Fragments arranged by size: 1712 1621 1443 511 411 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 6249 6281 2442 Size: 32 3775 32 2450 Fragments arranged by size: 3775 2450 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 2272 3625 2272 Size: 1353 4936 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 3614 1870 Size: 1744 4545 XmnI GAAnn'nnTTC Cuts at: 2747 3464 4418 2747 Size: 717 954 4618 Fragments arranged by size: 4618 954 717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI