(Linear) (Six-base) MAPSORT of: KIEE4504.seq Check: 2104 from: 1 to: 1584 KIEE4504.orf With 182 enzymes: SgfI * March 22, 2012 18:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 453 1584 Size: 453 1131 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 100 112 659 754 946 1584 Size: 41 59 12 547 95 192 638 Fragments arranged by size: 638 547 192 95 59 41 12 AfeI AGC'GCT Cuts at: 0 1014 1584 Size: 1014 570 AflII C'TTAA_G Cuts at: 0 811 1584 Size: 811 773 AflIII A'CryG_T Cuts at: 0 144 1397 1584 Size: 144 1253 187 Fragments arranged by size: 1253 187 144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 343 1163 1584 Size: 343 820 421 Fragments arranged by size: 820 421 343 AlwNI CAG_nnn'CTG Cuts at: 0 1381 1584 Size: 1381 203 ApoI r'AATT_y Cuts at: 0 110 930 1068 1487 1584 Size: 110 820 138 419 97 Fragments arranged by size: 820 419 138 110 97 BamHI G'GATC_C Cuts at: 0 963 1584 Size: 963 621 BanII G_rGCy'C Cuts at: 0 673 1442 1546 1584 Size: 673 769 104 38 Fragments arranged by size: 769 673 104 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 1452 1584 Size: 1452 132 BbvCI CC'TCA_GC Cuts at: 0 449 902 1584 Size: 449 453 682 Fragments arranged by size: 682 453 449 BclI T'GATC_A Cuts at: 0 299 558 1584 Size: 299 259 1026 Fragments arranged by size: 1026 299 259 BlpI GC'TnA_GC Cuts at: 0 373 1584 Size: 373 1211 Bme1580I G_kGCm'C Cuts at: 0 164 332 1232 1584 Size: 164 168 900 352 Fragments arranged by size: 900 352 168 164 BmgBI CAC'GTC Cuts at: 0 1179 1584 Size: 1179 405 BmrI ACTGGGnnnn_n' Cuts at: 0 126 1584 Size: 126 1458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 464 938 1584 Size: 334 130 474 646 Fragments arranged by size: 646 474 334 130 Bpu10I CC'TnA_GC Cuts at: 0 449 902 1584 Size: 449 453 682 Fragments arranged by size: 682 453 449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1426 1584 Size: 328 1098 158 Fragments arranged by size: 1098 328 158 BsaI GGTCTCn'nnnn_ Cuts at: 0 914 1584 Size: 914 670 BsaAI yAC'GTr Cuts at: 0 876 1398 1584 Size: 876 522 186 Fragments arranged by size: 876 522 186 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 437 467 1584 Size: 391 30 16 30 1117 Fragments arranged by size: 1117 391 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 399 697 1096 1310 1450 1510 1584 Size: 399 298 399 214 140 60 74 Fragments arranged by size: 399 399 298 214 140 74 60 BseYI C'CCAG_C Cuts at: 0 13 1539 1584 Size: 13 1526 45 Fragments arranged by size: 1526 45 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 16 584 1584 Size: 16 568 1000 Fragments arranged by size: 1000 568 16 BsiHKAI G_wGCw'C Cuts at: 0 473 673 1397 1442 1584 Size: 473 200 724 45 142 Fragments arranged by size: 724 473 200 142 45 Bsp1286I G_dGCh'C Cuts at: 0 164 332 473 673 1232 1397 1442 1546 Size: 164 168 141 200 559 165 45 104 Cuts at: 1546 1584 Size: 38 Fragments arranged by size: 559 200 168 165 164 141 104 45 38 BspHI T'CATG_A Cuts at: 0 1529 1584 Size: 1529 55 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 59 453 1584 Size: 59 394 1131 Fragments arranged by size: 1131 394 59 BsrBI CCG'CTC Cuts at: 0 99 1211 1584 Size: 99 1112 373 Fragments arranged by size: 1112 373 99 BsrDI GCAATG_nn' Cuts at: 0 314 1584 Size: 314 1270 BsrGI T'GTAC_A Cuts at: 0 398 909 1584 Size: 398 511 675 Fragments arranged by size: 675 511 398 BstAPI GCAn_nnn'nTGC Cuts at: 0 477 1381 1584 Size: 477 904 203 Fragments arranged by size: 904 477 203 BstYI r'GATC_y Cuts at: 0 963 1026 1584 Size: 963 63 558 Fragments arranged by size: 963 558 63 BtgI C'CryG_G Cuts at: 0 131 256 413 1195 1584 Size: 131 125 157 782 389 Fragments arranged by size: 782 389 157 131 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1488 1584 Size: 1488 96 DraI TTT'AAA Cuts at: 0 530 1584 Size: 530 1054 EaeI y'GGCC_r Cuts at: 0 1007 1049 1584 Size: 1007 42 535 Fragments arranged by size: 1007 535 42 EarI CTCTTCn'nnn_ Cuts at: 0 17 65 515 674 678 1584 Size: 17 48 450 159 4 906 Fragments arranged by size: 906 450 159 48 17 4 EcoICRI GAG'CTC Cuts at: 0 671 1440 1584 Size: 671 769 144 Fragments arranged by size: 769 671 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 100 112 334 464 659 754 938 Size: 41 59 12 222 130 195 95 184 Cuts at: 938 946 1584 Size: 8 638 Fragments arranged by size: 638 222 195 184 130 95 59 41 12 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 886 1373 1584 Size: 55 831 487 211 Fragments arranged by size: 831 487 211 55 EcoO109I rG'GnC_Cy Cuts at: 0 1057 1584 Size: 1057 527 EcoRI G'AATT_C Cuts at: 0 930 1068 1584 Size: 930 138 516 Fragments arranged by size: 930 516 138 EcoRV GAT'ATC Cuts at: 0 1107 1584 Size: 1107 477 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 759 1333 1476 1584 Size: 759 574 143 108 Fragments arranged by size: 759 574 143 108 HaeII r_GCGC'y Cuts at: 0 1016 1584 Size: 1016 568 MscI TGG'CCA Cuts at: 0 1009 1051 1584 Size: 1009 42 533 Fragments arranged by size: 1009 533 42 MslI CAynn'nnrTG Cuts at: 0 325 1129 1145 1584 Size: 325 804 16 439 Fragments arranged by size: 804 439 325 16 NcoI C'CATG_G Cuts at: 0 256 1584 Size: 256 1328 NspI r_CATG'y Cuts at: 0 148 799 1317 1584 Size: 148 651 518 267 Fragments arranged by size: 651 518 267 148 PciI A'CATG_T Cuts at: 0 144 1584 Size: 144 1440 PfoI T'CCnGG_A Cuts at: 0 645 915 971 1584 Size: 645 270 56 613 Fragments arranged by size: 645 613 270 56 PmlI CAC'GTG Cuts at: 0 1398 1584 Size: 1398 186 PstI C_TGCA'G Cuts at: 0 56 509 1247 1584 Size: 56 453 738 337 Fragments arranged by size: 738 453 337 56 SacI G_AGCT'C Cuts at: 0 673 1442 1584 Size: 673 769 142 Fragments arranged by size: 769 673 142 SapI GCTCTTCn'nnn_ Cuts at: 0 678 1584 Size: 678 906 SbfI CC_TGCA'GG Cuts at: 0 56 1584 Size: 56 1528 SfcI C'TryA_G Cuts at: 0 52 505 1243 1331 1584 Size: 52 453 738 88 253 Fragments arranged by size: 738 453 253 88 52 SmlI C'TyrA_G Cuts at: 0 343 811 1441 1584 Size: 343 468 630 143 Fragments arranged by size: 630 468 343 143 SphI G_CATG'C Cuts at: 0 1317 1584 Size: 1317 267 StuI AGG'CCT Cuts at: 0 742 1584 Size: 742 842 StyI C'CwwG_G Cuts at: 0 256 332 1232 1308 1584 Size: 256 76 900 76 276 Fragments arranged by size: 900 276 256 76 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1157 1584 Size: 1157 427 TatI w'GTAC_w Cuts at: 0 383 398 909 1584 Size: 383 15 511 675 Fragments arranged by size: 675 511 383 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 437 469 1584 Size: 437 32 1115 Fragments arranged by size: 1115 437 32 XbaI T'CTAG_A Cuts at: 0 267 1584 Size: 267 1317 XmnI GAAnn'nnTTC Cuts at: 0 742 1459 1584 Size: 742 717 125 Fragments arranged by size: 742 717 125 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AlfI AlwNI ApoI BamHI BanII BbsI BbvCI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstYI BtgI BtgZI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII MscI MslI NcoI NspI PciI PfoI PmlI PstI SacI SapI SbfI SfcI SmlI SphI StuI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI BfrBI BglI BglII BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI EagI EciI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XcmI XhoI XmaI ZraI