Restriction map for
pFN21AE3921
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsoBI Bsp1286I
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I
CelII CpoI DraII DraIII DrdI
DsaI Eam1105I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI FspI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NarI NcoI NdeI
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 51 CGCG CG^CG
AcyI 27 GACGCC G[GA]^CG[CT]C
Aor51HI 1161 AGCGCT AGC^GCT
AvaII 157 GGTCC G^G[AT]CC
BbvI 212 GCAGC GCAGC(8/12)
BcgI 185 CGAGGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 555 TGATCA T^GATCA
BcnI 160 CCGGG CC^[GC]GG
BpmI 1149 CTCCAG CTGGAG(16/14)
BsaHI 27 GACGCC G[GA]^CG[CT]C
BsaI 265 GAGACC GGTCTC(1/5)
BsgI 697 GTGCAG GTGCAG(16/14)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsmBI 25 GAGACG CGTCTC(1/5)
BsmI 1016 GCATTC GAATGC(1/-1)
BspDI 865 ATCGAT AT^CGAT
BspHI 1089 TCATGA T^CATGA
BsrDI 807 CATTGC GCAATG(2/0)
BsrFI 37 GCCGGC [GA]^CCGG[CT]
BstEII 1132 GGTCACC G^GT[ACGT]ACC
BstNI 352 CCTGG CC^[AT]GG
BstPI 1132 GGTCACC G^GT[ACGT]ACC
BstUI 51 CGCG CG^CG
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
ClaI 865 ATCGAT AT^CGAT
DraI 981 TTTAAA TTT^AAA
EaeI 53 CGGCCG [CT]^GGCC[GA]
EagI 53 CGGCCG C^GGCCG
EarI 100 GAAGAG CTCTTC(1/4)
EclXI 53 CGGCCG C^GGCCG
Eco47III 1161 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
EcoO65I 1132 GGTCACC G^GT[ACGT]ACC
EcoRII 352 CCTGG ^CC[AT]GG
EcoT14I 725 CCTTGG C^C[AT][AT]GG
FauI 118 GCGGG CCCGC(4/6)
FbaI 555 TGATCA T^GATCA
Hin1I 27 GACGCC G[GA]^CG[CT]C
HincII 499 GTTAAC GT[CT]^[GA]AC
HindII 499 GTTAAC GT[CT]^[GA]AC
HpaI 499 GTTAAC GTT^AAC
Ksp632I 100 GAAGAG CTCTTC(1/4)
MaeII 425 ACGT A^CGT
MspA1I 213 CAGCGG C[AC]G^C[GT]G
MvaI 352 CCTGG CC^[AT]GG
MvnI 51 CGCG CG^CG
NaeI 37 GCCGGC GCC^GGC
NciI 160 CCGGG CC^[GC]GG
NgoMIV 37 GCCGGC G^CCGGC
PflMI 231 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 203 GACTC GAGTC(4/5)
PstI 7 CTGCAG CTGCA^G
RcaI 1089 TCATGA T^CATGA
SfaNI 613 GATGC GCATC(5/9)
SfcI 7 CTGCAG C^T[GA][CT]AG
SspI 672 AATATT AAT^ATT
StyI 725 CCTTGG C^C[AT][AT]GG
TaqI 866 TCGA T^CGA
TseI 212 GCAGC G^C[AT]GC
TthHB8I 866 TCGA T^CGA
Van91I 231 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 227 CCATCCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 12 GTAC GT^AC
284 GTAC
AlwNI 611 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1122 CAGGAGCTG
BsmAI 25 GAGAC GTCTC(1/5)
265 GAGAC
BsrI 1008 ACTGG ACTGG(1/-1)
1151 CCAGT
BssKI 160 CCGGG ^CC[ACGT]GG
352 CCTGG
Cfr13I 157 GGTCC G^G[ACGT]CC
593 GGGCC
DdeI 637 CTTAG C^T[ACGT]AG
1021 CTAAG
HaeII 166 GGCGCT [GA]GCGC^[CT]
1161 AGCGCT
HaeIII 54 GGCC GG^CC
594 GGCC
HgaI 27 GACGC GACGC(5/10)
433 GACGC
HindIII 773 AAGCTT A^AGCTT
1032 AAGCTT
RsaI 12 GTAC GT^AC
284 GTAC
Sau96I 157 GGTCC G^G[ACGT]CC
593 GGGCC
ScrFI 160 CCGGG CC^[ACGT]GG
352 CCTGG
SpeI 652 ACTAGT A^CTAGT
1141 ACTAGT
TspRI 1007 CACTG CA[GC]TG[ACGT][ACGT]^
1069 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BsaJI BseRI BsiYI BslI BsmFI
BstF5I Cac8I CfoI DpnII Fnu4HI
FokI HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MwoI NdeII NlaIII Sau3AI TfiI
Tru9I Tsp45I Tsp509I