(Circular) (Six-base) MAPSORT of: pFN21AE3921.seq Check: 7898 from: 1 to: 5902 pFN21AE3921 5902 bp With 182 enzymes: SgfI * March 28, 2012 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2389 3965 5349 5617 1632 Size: 757 1576 1384 268 1917 Fragments arranged by size: 1917 1576 1384 757 268 AfeI AGC'GCT Cuts at: 3168 3168 Size: 5902 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3034 1184 Size: 1850 4052 AlwNI CAG_nnn'CTG Cuts at: 2621 3132 3645 5217 2621 Size: 511 513 1572 3306 Fragments arranged by size: 3306 1572 513 511 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2335 2518 2761 3002 3211 3437 3589 5698 Size: 842 183 243 241 209 226 152 2109 Cuts at: 5698 1493 Size: 1697 Fragments arranged by size: 2109 1697 842 243 241 226 209 183 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 3227 1870 Size: 73 1284 4545 Fragments arranged by size: 4545 1284 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 3221 1391 Size: 1830 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2179 2213 4093 4127 1903 Size: 34 242 34 1880 34 3678 Fragments arranged by size: 3678 1880 242 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 2560 1548 Size: 1012 4890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3139 4562 1239 Size: 1900 1423 2579 Fragments arranged by size: 2579 1900 1423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2264 4565 882 Size: 617 765 2301 2219 Fragments arranged by size: 2301 2219 765 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 3709 4091 4726 Size: 53 83 186 936 500 1676 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1676 1451 936 635 500 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3121 3151 1178 Size: 30 165 30 1718 30 3929 Fragments arranged by size: 3929 1718 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2111 2114 2123 1186 Size: 856 69 3 9 4965 Fragments arranged by size: 4965 856 69 9 3 BseYI C'CCAG_C Cuts at: 2621 5105 5601 2621 Size: 2484 496 2922 Fragments arranged by size: 2922 2484 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2723 1163 Size: 573 987 4342 Fragments arranged by size: 4342 987 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3352 4113 5141 1540 Size: 275 186 60 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 3021 3413 3506 3021 Size: 392 93 5417 Fragments arranged by size: 5417 392 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2024 2024 Size: 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3221 3963 4048 5119 1391 Size: 399 1431 742 85 1071 2174 Fragments arranged by size: 2174 1431 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3094 3791 5521 3094 Size: 697 1730 3475 Fragments arranged by size: 3475 1730 697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 4397 4571 65 Size: 1183 1563 1586 174 1396 Fragments arranged by size: 1586 1563 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 4546 4733 1700 Size: 110 232 2504 187 2869 Fragments arranged by size: 2869 2504 232 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 3137 1184 Size: 1953 3949 BstYI r'GATC_y Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897 Size: 1260 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5350 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 2871 1554 Size: 1317 4585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 2988 3206 2988 Size: 218 5684 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3349 4238 Size: 54 1425 50 275 18 228 1291 889 Cuts at: 4238 5867 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1291 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 3349 1537 Size: 275 246 1291 4090 Fragments arranged by size: 4090 1291 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 3832 1353 Size: 747 1732 3423 Fragments arranged by size: 3423 1732 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 2142 4489 4863 5009 387 Size: 1064 138 450 103 2347 374 146 1280 Fragments arranged by size: 2347 1280 1064 450 374 146 138 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2389 3139 3965 4562 5349 5617 1239 Size: 393 757 750 826 597 787 268 1524 Fragments arranged by size: 1524 826 787 757 750 597 393 268 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2175 3170 5049 1678 Size: 497 995 1879 2531 Fragments arranged by size: 2531 1879 995 497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 2828 2860 2927 Size: 32 135 32 965 32 259 32 67 Cuts at: 2927 2959 3121 3153 4543 4575 4617 4649 1373 Size: 32 162 32 1390 32 42 32 2626 Fragments arranged by size: 2626 1390 965 259 162 135 67 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3246 3492 669 Size: 988 281 568 740 246 3079 Fragments arranged by size: 3079 988 740 568 281 246 HindIII A'AGCT_T Cuts at: 748 2778 3037 748 Size: 2030 259 3613 Fragments arranged by size: 3613 2030 259 HpaI GTT'AAC Cuts at: 2506 3492 2506 Size: 986 4916 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 3318 3995 5143 5388 1606 Size: 614 1098 677 1148 245 2120 Fragments arranged by size: 2120 1148 1098 677 614 245 NaeI GCC'GGC Cuts at: 2044 2044 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5350 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NgoMIV G'CCGG_C Cuts at: 2042 2042 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 1108 Size: 1134 4768 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 2304 3472 2304 Size: 1168 4734 PstI C_TGCA'G Cuts at: 830 2016 3254 830 Size: 1186 1238 3478 Fragments arranged by size: 3478 1238 1186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2012 3250 4385 5066 5257 826 Size: 219 967 1238 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 967 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 2657 3146 152 Size: 2505 489 2908 Fragments arranged by size: 2908 2505 489 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2679 3826 5 Size: 47 2627 1147 2081 Fragments arranged by size: 2627 2081 1147 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2730 3341 513 Size: 552 1665 611 3074 Fragments arranged by size: 3074 1665 611 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 3101 3929 3946 4099 4284 4729 Size: 1255 698 823 828 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1498 1255 828 823 698 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2239 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI