Restriction map for
pFN21AE4508
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FseI FspI
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AflIII 560 ACATGT A^C[GA][CT]GT
AocI 747 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1129 AGCGCT AGC^GCT
Asp700I 1510 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1253 CTCGAG C^[CT]CG[GA]G
AvaII 858 GGACC G^G[AT]CC
BalI 929 TGGCCA TGG^CCA
BamHI 1327 GGATCC G^GATCC
BanI 493 GGCACC G^G[CT][GA]CC
BanII 914 GAGCTC G[GA]GC[CT]^C
BclI 1269 TGATCA T^GATCA
BseRI 482 CTCCTC GAGGAG(10/8)
BsmFI 1260 GGGAC GGGAC(10/14)
BsoBI 1253 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1242 TGTACA T^GTACA
BspLU11I 560 ACATGT A^CATGT
BspMI 849 ACCTGC ACCTGC(4/8)
BsrDI 1304 CATTGC GCAATG(2/0)
BsrGI 1242 TGTACA T^GTACA
BssSI 173 CTCGTG CACGAG(-5/-1)
BstBI 188 TTCGAA TT^CGAA
BstEII 252 GGTGACC G^GT[ACGT]ACC
BstPI 252 GGTGACC G^GT[ACGT]ACC
Bsu36I 747 CCTAAGG CC^T[ACGT]AGG
CfoI 1130 GCGC GCG^C
Cfr13I 858 GGACC G^G[ACGT]CC
DrdI 4 GACGGAATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1129 AGCGCT AGC^GCT
Eco81I 747 CCTAAGG CC^T[ACGT]AGG
EcoO65I 252 GGTGACC G^GT[ACGT]ACC
FbaI 1269 TGATCA T^GATCA
HaeII 1129 AGCGCT [GA]GCGC^[CT]
HhaI 1130 GCGC GCG^C
HinP1I 1130 GCGC G^CGC
HincII 1587 GTCAAC GT[CT]^[GA]AC
HindII 1587 GTCAAC GT[CT]^[GA]AC
HindIII 659 AAGCTT A^AGCTT
MaeII 432 ACGT A^CGT
MluNI 929 TGGCCA TGG^CCA
MscI 929 TGGCCA TGG^CCA
NspI 560 ACATGT [GA]CATG^[CT]
NspV 188 TTCGAA TT^CGAA
PaeR7I 1253 CTCGAG C^TCGAG
PshAI 459 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 914 GAGCTC GAGCT^C
SapI 1483 GCTCTTC GCTCTTC(1/4)
Sau96I 858 GGACC G^G[ACGT]CC
SfaNI 1308 GCATC GCATC(5/9)
SfuI 188 TTCGAA TT^CGAA
SmlI 1253 CTCGAG C^T[CT][GA]AG
SspBI 1242 TGTACA T^GTACA
StuI 1520 AGGCCT AGG^CCT
SwaI 1031 ATTTAAAT ATTT^AAAT
XhoI 1253 CTCGAG C^TCGAG
XmnI 1510 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsgI 512 GTGCAG GTGCAG(16/14)
1093 GTGCAG
BsiYI 484 CCTCTTAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1252 CCTCGAGAGGG
BslI 484 CCTCTTAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1252 CCTCGAGAGGG
BsmAI 889 GAGAC GTCTC(1/5)
994 GTCTC
Cac8I 736 GCCTGC GC[ACGT]^[ACGT]GC
970 GCTGGC
DraI 139 TTTAAA TTT^AAA
1032 TTTAAA
EaeI 929 TGGCCA [CT]^GGCC[GA]
972 TGGCCG
EcoT14I 884 CCAAGG C^C[AT][AT]GG
1548 CCTTGG
MslI 261 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1180 CATCTTAATG
ScaI 386 AGTACT AGT^ACT
537 AGTACT
SspI 597 AATATT AAT^ATT
724 AATATT
StyI 884 CCAAGG C^C[AT][AT]GG
1548 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI BbvI BfaI BmyI
BpmI BsaJI BsiHKAI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI ScrFI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII