(Circular) (Six-base) MAPSORT of: pFN21AE4508.seq Check: 2973 from: 1 to: 6301 pFN21AE4508 6301 bp With 182 enzymes: SgfI * March 28, 2012 14:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 3479 4364 5748 6016 1632 Size: 612 1235 885 1384 268 1917 Fragments arranged by size: 1917 1384 1235 885 612 268 AfeI AGC'GCT Cuts at: 3136 3136 Size: 6301 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2565 5200 6177 1203 Size: 1362 2635 977 1327 Fragments arranged by size: 2635 1362 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2222 2960 2963 2972 4044 5616 2222 Size: 738 3 9 1072 1572 2907 Fragments arranged by size: 2907 1572 1072 738 9 3 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2141 2779 3340 3610 3836 3988 6097 1493 Size: 648 638 561 270 226 152 2109 1697 Fragments arranged by size: 2109 1697 648 638 561 270 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3258 3626 1870 Size: 73 1315 368 4545 Fragments arranged by size: 4545 1315 368 73 BamHI G'GATC_C Cuts at: 3332 3631 3998 3332 Size: 299 367 5635 Fragments arranged by size: 5635 367 299 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2498 3622 5077 618 Size: 325 217 627 711 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 711 627 325 217 BanII G_rGCy'C Cuts at: 1391 2923 3620 1391 Size: 1532 697 4072 Fragments arranged by size: 4072 1532 697 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 3274 1548 Size: 1726 4575 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 3446 4362 5518 1790 Size: 1656 916 1156 2573 Fragments arranged by size: 2573 1656 1156 916 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 4032 4990 Size: 416 53 83 399 24 2030 585 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 2030 1753 958 585 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 2657 3015 4961 1239 Size: 1272 146 358 1946 2579 Fragments arranged by size: 2579 1946 1272 358 146 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 5291 5589 4426 Size: 865 298 5138 Fragments arranged by size: 5138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4964 882 Size: 617 3465 2219 Fragments arranged by size: 3465 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6076 Fragments arranged by size: 6076 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 1186 Size: 1292 5009 BseYI C'CCAG_C Cuts at: 2866 5504 6000 2866 Size: 2638 496 3167 Fragments arranged by size: 3167 2638 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2538 3119 1163 Size: 573 802 581 4345 Fragments arranged by size: 4345 802 581 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2173 2443 2923 4362 4447 5518 2173 Size: 270 480 1439 85 1071 2956 Fragments arranged by size: 2956 1439 1071 480 270 85 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 2443 2923 3446 3620 4362 4447 Size: 399 383 270 480 523 174 742 85 Cuts at: 4447 5518 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 523 480 399 383 270 174 85 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2863 3656 844 Size: 2019 793 3489 Fragments arranged by size: 3489 2019 793 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3308 4796 4970 65 Size: 1183 2060 1488 174 1396 Fragments arranged by size: 2060 1488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3247 96 Size: 3151 3150 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 2178 4361 5373 2178 Size: 2183 1012 3106 Fragments arranged by size: 3106 2183 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2885 2960 2963 2972 794 Size: 2091 75 3 9 4123 Fragments arranged by size: 4123 2091 75 9 3 BstBI TT'CG_AA Cuts at: 2194 5105 2194 Size: 2911 3390 BstEII G'GTnAC_C Cuts at: 1184 2257 1184 Size: 1073 5228 BstYI r'GATC_y Cuts at: 1972 2048 3332 3412 3631 3998 4383 4400 5852 Size: 76 1284 80 219 367 385 17 1452 Cuts at: 5852 5938 5950 6296 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 219 86 80 76 17 12 Bsu36I CC'TnA_GG Cuts at: 2753 2753 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2077 2526 3817 4611 4631 2077 Size: 449 1291 794 20 3747 Fragments arranged by size: 3747 1291 794 449 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 2146 3039 3605 2146 Size: 893 566 4842 Fragments arranged by size: 4842 893 566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 5308 809 Size: 507 699 3293 1802 Fragments arranged by size: 3293 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2934 2977 3748 Size: 54 1425 50 275 18 1104 43 771 Cuts at: 3748 4637 6266 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1104 889 771 275 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 3039 3083 3402 3495 4231 1353 Size: 683 1003 44 319 93 736 3423 Fragments arranged by size: 3423 1003 736 683 319 93 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2921 2921 Size: 6301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2511 2657 3015 3479 4364 4961 Size: 393 612 267 146 358 464 885 597 Cuts at: 4961 5748 6016 1239 Size: 787 268 1524 Fragments arranged by size: 1524 885 787 612 597 464 393 358 268 267 146 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 3138 5448 1678 Size: 1460 2310 2531 Fragments arranged by size: 2531 2310 1460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 2822 2854 4942 Size: 32 135 32 775 32 443 32 2088 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2088 775 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3645 3891 669 Size: 988 281 1656 51 246 3079 Fragments arranged by size: 3079 1656 988 281 246 51 HindIII A'AGCT_T Cuts at: 748 2664 748 Size: 1916 4385 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3597 3900 3597 Size: 303 5998 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5415 5599 1305 Size: 4110 184 2007 Fragments arranged by size: 4110 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2936 10 Size: 54 1425 343 1104 3375 Fragments arranged by size: 3375 1425 1104 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 3189 4259 4618 4777 518 Size: 648 1104 919 1070 359 159 2042 Fragments arranged by size: 2042 1104 1070 919 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2468 2960 2969 3005 3717 4394 5542 5787 Size: 862 492 9 36 712 677 1148 245 Cuts at: 5787 1606 Size: 2120 Fragments arranged by size: 2120 1148 862 712 677 492 245 36 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2569 3659 5204 1132 Size: 97 1340 1090 1545 2229 Fragments arranged by size: 2229 1545 1340 1090 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 2565 5200 2565 Size: 2635 3666 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PfoI T'CCnGG_A Cuts at: 2914 2914 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2468 1613 Size: 855 5446 PsiI TTA'TAA Cuts at: 2736 3549 3871 2736 Size: 813 322 5166 Fragments arranged by size: 5166 813 322 PstI C_TGCA'G Cuts at: 830 2881 2956 2968 3653 830 Size: 2051 75 12 685 3478 Fragments arranged by size: 3478 2051 685 75 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2468 2960 2969 3005 2468 Size: 492 9 36 5764 Fragments arranged by size: 5764 492 36 9 SacI G_AGCT'C Cuts at: 2923 2923 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SapI GCTCTTCn'nnn_ Cuts at: 3495 3495 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 2393 2544 4549 6261 2393 Size: 151 2005 1712 2433 Fragments arranged by size: 2433 2005 1712 151 SfcI C'TryA_G Cuts at: 826 1045 2877 2952 2964 3649 4784 5465 5656 Size: 219 1832 75 12 685 1135 681 191 Cuts at: 5656 826 Size: 1471 Fragments arranged by size: 1832 1471 1135 685 681 219 191 75 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3258 4405 5306 5568 820 Size: 197 926 1315 1147 901 262 1553 Fragments arranged by size: 1553 1315 1147 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 2604 2731 4225 5 Size: 47 2552 127 1494 2081 Fragments arranged by size: 2552 2081 1494 127 47 StuI AGG'CCT Cuts at: 1517 3527 1517 Size: 2010 4291 StyI C'CwwG_G Cuts at: 513 1065 2004 2889 3553 3740 513 Size: 552 939 885 664 187 3074 Fragments arranged by size: 3074 939 885 664 552 187 SwaI ATTT'AAAT Cuts at: 3039 3039 Size: 6301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2182 2391 2542 Size: 274 80 33 51 1443 205 209 151 Cuts at: 2542 2568 3247 4547 6259 96 Size: 26 679 1300 1712 138 Fragments arranged by size: 1712 1443 1300 679 274 209 205 151 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 3258 1943 Size: 1315 4986 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 3519 4430 3519 Size: 911 5390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI