Restriction map for
pFN21AE4508
(Linear) (Six-base) MAPSORT of: KIEE4508.seq Check: 3444 from: 1 to: 1596
KIEE4508.orf
With 182 enzymes: SgfI *
March 22, 2012 18:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1474 1596
Size: 239 1235 122
Fragments arranged by size:
1235 239 122
AfeI AGC'GCT
Cuts at: 0 1131 1596
Size: 1131 465
AflIII A'CryG_T
Cuts at: 0 560 1596
Size: 560 1036
AlwNI CAG_nnn'CTG
Cuts at: 0 217 955 958 967 1596
Size: 217 738 3 9 629
Fragments arranged by size:
738 629 217 9 3
ApoI r'AATT_y
Cuts at: 0 136 774 1335 1596
Size: 136 638 561 261
Fragments arranged by size:
638 561 261 136
AvaI C'yCGr_G
Cuts at: 0 1253 1596
Size: 1253 343
BamHI G'GATC_C
Cuts at: 0 1327 1596
Size: 1327 269
BanI G'GyrC_C
Cuts at: 0 493 1596
Size: 493 1103
BanII G_rGCy'C
Cuts at: 0 918 1596
Size: 918 678
BclI T'GATC_A
Cuts at: 0 1269 1596
Size: 1269 327
Bme1580I G_kGCm'C
Cuts at: 0 1441 1596
Size: 1441 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 1442 1596
Size: 1442 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 652 1010 1596
Size: 506 146 358 586
Fragments arranged by size:
586 506 358 146
Bpu10I CC'TnA_GC
Cuts at: 0 437 1596
Size: 437 1159
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1596
Size: 473 1123
BseYI C'CCAG_C
Cuts at: 0 861 1596
Size: 861 735
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1114 1596
Size: 533 581 482
Fragments arranged by size:
581 533 482
BsiHKAI G_wGCw'C
Cuts at: 0 168 438 918 1596
Size: 168 270 480 678
Fragments arranged by size:
678 480 270 168
Bsp1286I G_dGCh'C
Cuts at: 0 168 438 918 1441 1596
Size: 168 270 480 523 155
Fragments arranged by size:
523 480 270 168 155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 1596
Size: 858 738
BsrDI GCAATG_nn'
Cuts at: 0 1303 1596
Size: 1303 293
BsrGI T'GTAC_A
Cuts at: 0 1242 1596
Size: 1242 354
BssSI C'ACGA_G
Cuts at: 0 173 1596
Size: 173 1423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 880 955 958 967 1596
Size: 880 75 3 9 629
Fragments arranged by size:
880 629 75 9 3
BstBI TT'CG_AA
Cuts at: 0 189 1596
Size: 189 1407
BstEII G'GTnAC_C
Cuts at: 0 252 1596
Size: 252 1344
BstYI r'GATC_y
Cuts at: 0 43 1327 1407 1596
Size: 43 1284 80 189
Fragments arranged by size:
1284 189 80 43
Bsu36I CC'TnA_GG
Cuts at: 0 748 1596
Size: 748 848
BtsI GCAGTG_nn'
Cuts at: 0 72 521 1596
Size: 72 449 1075
Fragments arranged by size:
1075 449 72
DraI TTT'AAA
Cuts at: 0 141 1034 1596
Size: 141 893 562
Fragments arranged by size:
893 562 141
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 1596
Size: 10 1586
EaeI y'GGCC_r
Cuts at: 0 929 972 1596
Size: 929 43 624
Fragments arranged by size:
929 624 43
EarI CTCTTCn'nnn_
Cuts at: 0 31 1034 1078 1397 1490 1596
Size: 31 1003 44 319 93 106
Fragments arranged by size:
1003 319 106 93 44 31
EcoICRI GAG'CTC
Cuts at: 0 916 1596
Size: 916 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 506 652 1010 1474 1596
Size: 239 267 146 358 464 122
Fragments arranged by size:
464 358 267 239 146 122
HaeII r_GCGC'y
Cuts at: 0 1133 1596
Size: 1133 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 817 849 1596
Size: 342 32 443 32 747
Fragments arranged by size:
747 443 342 32 32
HincII GTy'rAC
Cuts at: 0 1589 1596
Size: 1589 7
HindIII A'AGCT_T
Cuts at: 0 659 1596
Size: 659 937
MscI TGG'CCA
Cuts at: 0 931 1596
Size: 931 665
MslI CAynn'nnrTG
Cuts at: 0 265 1184 1596
Size: 265 919 412
Fragments arranged by size:
919 412 265
MspA1I CmG'CkG
Cuts at: 0 463 955 964 1000 1596
Size: 463 492 9 36 596
Fragments arranged by size:
596 492 463 36 9
NspI r_CATG'y
Cuts at: 0 564 1596
Size: 564 1032
PciI A'CATG_T
Cuts at: 0 560 1596
Size: 560 1036
PfoI T'CCnGG_A
Cuts at: 0 909 1596
Size: 909 687
PshAI GACnn'nnGTC
Cuts at: 0 463 1596
Size: 463 1133
PsiI TTA'TAA
Cuts at: 0 731 1544 1596
Size: 731 813 52
Fragments arranged by size:
813 731 52
PstI C_TGCA'G
Cuts at: 0 876 951 963 1596
Size: 876 75 12 633
Fragments arranged by size:
876 633 75 12
PvuII CAG'CTG
Cuts at: 0 463 955 964 1000 1596
Size: 463 492 9 36 596
Fragments arranged by size:
596 492 463 36 9
SacI G_AGCT'C
Cuts at: 0 918 1596
Size: 918 678
SapI GCTCTTCn'nnn_
Cuts at: 0 1490 1596
Size: 1490 106
ScaI AGT'ACT
Cuts at: 0 388 539 1596
Size: 388 151 1057
Fragments arranged by size:
1057 388 151
SfcI C'TryA_G
Cuts at: 0 872 947 959 1596
Size: 872 75 12 637
Fragments arranged by size:
872 637 75 12
SmlI C'TyrA_G
Cuts at: 0 1253 1596
Size: 1253 343
SspI AAT'ATT
Cuts at: 0 599 726 1596
Size: 599 127 870
Fragments arranged by size:
870 599 127
StuI AGG'CCT
Cuts at: 0 1522 1596
Size: 1522 74
StyI C'CwwG_G
Cuts at: 0 884 1548 1596
Size: 884 664 48
Fragments arranged by size:
884 664 48
SwaI ATTT'AAAT
Cuts at: 0 1034 1596
Size: 1034 562
TatI w'GTAC_w
Cuts at: 0 177 386 537 563 1242 1596
Size: 177 209 151 26 679 354
Fragments arranged by size:
679 354 209 177 151 26
XhoI C'TCGA_G
Cuts at: 0 1253 1596
Size: 1253 343
XmnI GAAnn'nnTTC
Cuts at: 0 1514 1596
Size: 1514 82
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AvaI BamHI BanI
BanII BclI Bme1580I BmrI BpmI Bpu10I BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtsI DraI DrdI EaeI
EarI EcoICRI Eco57MI HaeII Hin4I HincII HindIII MscI
MslI MspA1I NspI PciI PfoI PshAI PsiI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI SwaI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraIII EagI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
TaqII TstI Tth111I XbaI XcmI XmaI ZraI