(Circular) (Six-base) MAPSORT of: pFN21AE4537.seq Check: 583 from: 1 to: 6520 pFN21AE4537 6520 bp With 182 enzymes: SgfI * March 28, 2012 14:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3863 1937 Size: 1926 4594 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2130 2839 4583 5967 6235 1632 Size: 498 709 1744 1384 268 1917 Fragments arranged by size: 1917 1744 1384 709 498 268 AflII C'TTAA_G Cuts at: 820 1017 3667 820 Size: 197 2650 3673 Fragments arranged by size: 3673 2650 197 AflIII A'CryG_T Cuts at: 1203 5419 6396 1203 Size: 4216 977 1327 Fragments arranged by size: 4216 1327 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 2574 1166 Size: 1408 5112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AlwNI CAG_nnn'CTG Cuts at: 4263 5835 4263 Size: 1572 4948 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2598 2864 3295 3346 3538 3829 4055 4207 Size: 1105 266 431 51 192 291 226 152 Cuts at: 4207 6316 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1105 431 291 266 226 192 152 51 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 2194 2220 2352 3375 3845 1870 Size: 73 251 26 132 1023 470 4545 Fragments arranged by size: 4545 1023 470 251 132 73 26 BamHI G'GATC_C Cuts at: 3277 3850 4217 3277 Size: 573 367 5580 Fragments arranged by size: 5580 573 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2263 3841 5296 618 Size: 325 217 627 476 1578 1455 1842 Fragments arranged by size: 1842 1578 1455 627 476 325 217 BanII G_rGCy'C Cuts at: 1391 2097 3839 1391 Size: 706 1742 4072 Fragments arranged by size: 4072 1742 706 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 2495 928 Size: 971 130 466 4953 Fragments arranged by size: 4953 971 466 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3453 4412 5622 3453 Size: 959 1210 4351 Fragments arranged by size: 4351 1210 959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2158 2225 2261 Size: 107 122 71 631 605 486 67 36 Cuts at: 2261 5131 136 Size: 2870 1525 Fragments arranged by size: 2870 1525 631 605 486 122 107 71 67 36 BglII A'GATC_T Cuts at: 6515 6515 Size: 6520 BlpI GC'TnA_GC Cuts at: 3687 3937 3687 Size: 250 6270 Bme1580I G_kGCm'C Cuts at: 1790 2325 4581 5737 1790 Size: 535 2256 1156 2573 Fragments arranged by size: 2573 2256 1156 535 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4251 5209 442 Size: 416 53 83 399 24 2834 958 1753 Fragments arranged by size: 2834 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3097 3117 3290 5180 1239 Size: 1858 20 173 1890 2579 Fragments arranged by size: 2579 1890 1858 173 20 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 6520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4645 5510 5808 4645 Size: 865 298 5357 Fragments arranged by size: 5357 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5183 882 Size: 617 3684 2219 Fragments arranged by size: 3684 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2572 4311 493 Size: 711 1368 1739 2702 Fragments arranged by size: 2702 1739 1368 711 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2247 4327 4709 5344 Size: 53 83 186 936 714 2080 382 635 Cuts at: 5344 275 Size: 1451 Fragments arranged by size: 2080 1451 936 714 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4953 5351 5625 5772 6183 4953 Size: 398 274 147 411 5290 Fragments arranged by size: 5290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 2348 2378 3070 Size: 30 165 30 616 30 299 30 692 Cuts at: 3070 3100 1178 Size: 30 4598 Fragments arranged by size: 4598 692 616 299 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3017 3287 1186 Size: 1831 270 4419 Fragments arranged by size: 4419 1831 270 BseYI C'CCAG_C Cuts at: 2023 2206 5723 6219 2023 Size: 183 3517 496 2324 Fragments arranged by size: 3517 2324 496 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5947 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2162 2172 3970 4731 5759 Size: 275 186 134 27 10 1798 761 1028 Cuts at: 5759 1540 Size: 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 134 27 10 BsiHKAI G_wGCw'C Cuts at: 2361 2786 2827 3688 4581 4666 5737 2361 Size: 425 41 861 893 85 1071 3144 Fragments arranged by size: 3144 1071 893 861 425 85 41 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2325 2361 2786 2827 3688 3839 Size: 399 307 228 36 425 41 861 151 Cuts at: 3839 4581 4666 5737 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 861 742 425 399 307 228 151 85 41 36 BspEI T'CCGG_A Cuts at: 6183 6183 Size: 6520 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3322 3875 844 Size: 2478 553 3489 Fragments arranged by size: 3489 2478 553 BsrBI CCG'CTC Cuts at: 1394 1823 4407 1394 Size: 429 2584 3507 Fragments arranged by size: 3507 2584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3337 5015 5189 65 Size: 1183 2089 1678 174 1396 Fragments arranged by size: 2089 1678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2167 2260 2407 5164 5351 1700 Size: 110 357 93 147 2757 187 2869 Fragments arranged by size: 2869 2757 357 187 147 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 2348 3088 794 Size: 1554 740 4226 Fragments arranged by size: 4226 1554 740 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6520 BstYI r'GATC_y Cuts at: 1972 3277 3412 3850 4217 4602 4619 6071 6157 Size: 1305 135 438 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1305 438 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2126 2156 2295 3281 513 Size: 552 939 122 30 139 986 3752 Fragments arranged by size: 3752 986 939 552 139 122 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5781 Fragments arranged by size: 5781 683 56 BtsI GCAGTG_nn' Cuts at: 4036 4830 4850 4036 Size: 794 20 5706 Fragments arranged by size: 5706 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3607 3824 3607 Size: 217 6303 DraIII CAC_nnn'GTG Cuts at: 2831 2831 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2894 5527 809 Size: 507 1578 2633 1802 Fragments arranged by size: 2633 1802 1578 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2159 2169 2483 Size: 54 1425 50 275 18 329 10 314 Cuts at: 2483 3284 3559 3967 4856 6485 8 Size: 801 275 408 889 1629 43 Fragments arranged by size: 1629 1425 889 801 408 329 314 275 275 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2159 2169 3967 1537 Size: 275 347 10 1798 4090 Fragments arranged by size: 4090 1798 347 275 10 EarI CTCTTCn'nnn_ Cuts at: 1353 3615 4450 1353 Size: 2262 835 3423 Fragments arranged by size: 3423 2262 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 5107 5481 5627 387 Size: 1064 138 903 2615 374 146 1280 Fragments arranged by size: 2615 1280 1064 903 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2839 3097 3117 3290 4583 5180 Size: 393 498 709 258 20 173 1293 597 Cuts at: 5180 5967 6235 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1293 787 709 597 498 393 268 258 173 20 EcoO109I rG'GnC_Cy Cuts at: 2253 2253 Size: 6520 EcoRI G'AATT_C Cuts at: 2598 3295 3829 4207 2598 Size: 697 534 378 4911 Fragments arranged by size: 4911 697 534 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 6520 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 5667 1678 Size: 3989 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 2670 2702 2739 Size: 32 135 32 447 32 619 32 37 Cuts at: 2739 2771 2966 2998 3369 3401 3538 3570 5161 Size: 32 195 32 371 32 137 32 1591 Cuts at: 5161 5193 5235 5267 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1591 619 447 371 195 137 135 42 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3864 4110 669 Size: 988 281 1614 312 246 3079 Fragments arranged by size: 3079 1614 988 312 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6520 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 3489 4119 3489 Size: 630 5890 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 3620 5634 5818 1305 Size: 762 1553 2014 184 2007 Fragments arranged by size: 2014 2007 1553 762 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2485 3286 3561 10 Size: 54 1425 343 653 801 275 2969 Fragments arranged by size: 2969 1425 801 653 343 275 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2574 2984 3524 4478 4837 4996 Size: 648 1389 19 410 540 954 359 159 Cuts at: 4996 518 Size: 2042 Fragments arranged by size: 2042 1389 954 648 540 410 359 159 19 MspA1I CmG'CkG Cuts at: 1606 2128 2158 2210 2274 3075 3365 3936 4613 Size: 522 30 52 64 801 290 571 677 Cuts at: 4613 5761 6006 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 801 677 571 522 290 245 64 52 30 NaeI GCC'GGC Cuts at: 2169 2262 2409 2169 Size: 93 147 6280 Fragments arranged by size: 6280 147 93 NcoI C'CATG_G Cuts at: 513 1065 2004 3281 513 Size: 552 939 1277 3752 Fragments arranged by size: 3752 1277 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NgoMIV G'CCGG_C Cuts at: 2167 2260 2407 2167 Size: 93 147 6280 Fragments arranged by size: 6280 147 93 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NruI TCG'CGA Cuts at: 2232 2232 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 3878 5423 1132 Size: 97 2649 1545 2229 Fragments arranged by size: 2649 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6520 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6520 PfoI T'CCnGG_A Cuts at: 2241 2241 Size: 6520 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PstI C_TGCA'G Cuts at: 830 3872 830 Size: 3042 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 3365 3365 Size: 6520 SacII CC_GC'GG Cuts at: 2129 2159 2129 Size: 30 6490 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 3868 5003 5684 5875 826 Size: 219 2823 1135 681 191 1471 Fragments arranged by size: 2823 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 2354 3847 1872 Size: 482 1493 4545 Fragments arranged by size: 4545 1493 482 SmlI C'TyrA_G Cuts at: 820 1017 1943 3375 3667 4624 5525 5787 820 Size: 197 926 1432 292 957 901 262 1553 Fragments arranged by size: 1553 1432 957 926 901 292 262 197 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 3107 152 Size: 2955 3565 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6520 StyI C'CwwG_G Cuts at: 513 1065 2004 2670 3281 3287 3388 3959 513 Size: 552 939 666 611 6 101 571 3074 Fragments arranged by size: 3074 939 666 611 571 552 101 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3011 4547 4564 4717 4902 5347 325 Size: 1255 1431 1536 17 153 185 445 1498 Fragments arranged by size: 1536 1498 1431 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 2061 4766 Size: 274 80 33 51 1443 39 45 2705 Cuts at: 4766 6478 96 Size: 1712 138 Fragments arranged by size: 2705 1712 1443 274 138 80 51 45 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 6480 6512 2348 Size: 32 4100 32 2356 Fragments arranged by size: 4100 2356 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2624 1316 Size: 1308 5212 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3770 3770 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 3375 1943 Size: 1432 5088 XmaI C'CCGG_G Cuts at: 1870 2352 3845 1870 Size: 482 1493 4545 Fragments arranged by size: 4545 1493 482 XmnI GAAnn'nnTTC Cuts at: 2390 4649 2390 Size: 2259 4261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI