(Linear) (Six-base) MAPSORT of: KIEE4537.seq Check: 1520 from: 1 to: 1815 KIEE4537.orf With 182 enzymes: SgfI * March 22, 2012 18:18 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 834 1815 Size: 125 709 981 Fragments arranged by size: 981 709 125 AflII C'TTAA_G Cuts at: 0 1662 1815 Size: 1662 153 AleI CACnn'nnGTG Cuts at: 0 569 1815 Size: 569 1246 ApoI r'AATT_y Cuts at: 0 593 859 1290 1341 1533 1815 Size: 593 266 431 51 192 282 Fragments arranged by size: 593 431 282 266 192 51 AvaI C'yCGr_G Cuts at: 0 189 215 347 1370 1815 Size: 189 26 132 1023 445 Fragments arranged by size: 1023 445 189 132 26 BamHI G'GATC_C Cuts at: 0 1272 1815 Size: 1272 543 BanI G'GyrC_C Cuts at: 0 258 1815 Size: 258 1557 BanII G_rGCy'C Cuts at: 0 92 1815 Size: 92 1723 BbsI GAAGACnn'nnnn_ Cuts at: 0 24 490 1815 Size: 24 466 1325 Fragments arranged by size: 1325 466 24 BciVI GTATCCnnnnn_n' Cuts at: 0 1448 1815 Size: 1448 367 BglI GCCn_nnn'nGGC Cuts at: 0 153 220 256 1815 Size: 153 67 36 1559 Fragments arranged by size: 1559 153 67 36 BlpI GC'TnA_GC Cuts at: 0 1682 1815 Size: 1682 133 Bme1580I G_kGCm'C Cuts at: 0 320 1815 Size: 320 1495 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1092 1112 1285 1815 Size: 1092 20 173 530 Fragments arranged by size: 1092 530 173 20 Bpu10I CC'TnA_GC Cuts at: 0 407 1815 Size: 407 1408 BsaAI yAC'GTr Cuts at: 0 567 1815 Size: 567 1248 BsaHI Gr'CG_yC Cuts at: 0 242 1815 Size: 242 1573 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 14 44 343 373 1065 1095 1815 Size: 14 30 299 30 692 30 720 Fragments arranged by size: 720 692 299 30 30 30 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1012 1282 1815 Size: 1012 270 533 Fragments arranged by size: 1012 533 270 BseYI C'CCAG_C Cuts at: 0 18 201 1815 Size: 18 183 1614 Fragments arranged by size: 1614 183 18 BsiEI CG_ry'CG Cuts at: 0 130 157 167 1815 Size: 130 27 10 1648 Fragments arranged by size: 1648 130 27 10 BsiHKAI G_wGCw'C Cuts at: 0 356 781 822 1683 1815 Size: 356 425 41 861 132 Fragments arranged by size: 861 425 356 132 41 Bsp1286I G_dGCh'C Cuts at: 0 92 320 356 781 822 1683 1815 Size: 92 228 36 425 41 861 132 Fragments arranged by size: 861 425 228 132 92 41 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1317 1815 Size: 1317 498 BsrDI GCAATG_nn' Cuts at: 0 1332 1815 Size: 1332 483 BsrFI r'CCGG_y Cuts at: 0 162 255 402 1815 Size: 162 93 147 1413 Fragments arranged by size: 1413 162 147 93 BstAPI GCAn_nnn'nTGC Cuts at: 0 343 1083 1815 Size: 343 740 732 Fragments arranged by size: 740 732 343 BstYI r'GATC_y Cuts at: 0 1272 1407 1815 Size: 1272 135 408 Fragments arranged by size: 1272 408 135 BtgI C'CryG_G Cuts at: 0 121 151 290 1276 1815 Size: 121 30 139 986 539 Fragments arranged by size: 986 539 139 121 30 DraI TTT'AAA Cuts at: 0 1602 1815 Size: 1602 213 DraIII CAC_nnn'GTG Cuts at: 0 826 1815 Size: 826 989 DrdI GACnn_nn'nnGTC Cuts at: 0 889 1815 Size: 889 926 EaeI y'GGCC_r Cuts at: 0 154 164 478 1279 1554 1815 Size: 154 10 314 801 275 261 Fragments arranged by size: 801 314 275 261 154 10 EagI C'GGCC_G Cuts at: 0 154 164 1815 Size: 154 10 1651 Fragments arranged by size: 1651 154 10 EarI CTCTTCn'nnn_ Cuts at: 0 1610 1815 Size: 1610 205 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 487 1815 Size: 487 1328 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 834 1092 1112 1285 1815 Size: 125 709 258 20 173 530 Fragments arranged by size: 709 530 258 173 125 20 EcoO109I rG'GnC_Cy Cuts at: 0 248 1815 Size: 248 1567 EcoRI G'AATT_C Cuts at: 0 593 1290 1815 Size: 593 697 525 Fragments arranged by size: 697 593 525 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 633 1815 Size: 633 1182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 14 46 665 697 734 766 961 993 Size: 14 32 619 32 37 32 195 32 Cuts at: 993 1364 1396 1533 1565 1815 Size: 371 32 137 32 250 Fragments arranged by size: 619 371 250 195 137 37 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 0 1547 1815 Size: 1547 268 MfeI C'AATT_G Cuts at: 0 1484 1815 Size: 1484 331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 62 1615 1815 Size: 62 1553 200 Fragments arranged by size: 1553 200 62 MscI TGG'CCA Cuts at: 0 480 1281 1556 1815 Size: 480 801 275 259 Fragments arranged by size: 801 480 275 259 MslI CAynn'nnrTG Cuts at: 0 550 569 979 1519 1815 Size: 550 19 410 540 296 Fragments arranged by size: 550 540 410 296 19 MspA1I CmG'CkG Cuts at: 0 123 153 205 269 1070 1360 1815 Size: 123 30 52 64 801 290 455 Fragments arranged by size: 801 455 290 123 64 52 30 NaeI GCC'GGC Cuts at: 0 164 257 404 1815 Size: 164 93 147 1411 Fragments arranged by size: 1411 164 147 93 NcoI C'CATG_G Cuts at: 0 1276 1815 Size: 1276 539 NgoMIV G'CCGG_C Cuts at: 0 162 255 402 1815 Size: 162 93 147 1413 Fragments arranged by size: 1413 162 147 93 NruI TCG'CGA Cuts at: 0 227 1815 Size: 227 1588 PfoI T'CCnGG_A Cuts at: 0 236 1815 Size: 236 1579 PvuII CAG'CTG Cuts at: 0 1360 1815 Size: 1360 455 SacII CC_GC'GG Cuts at: 0 124 154 1815 Size: 124 30 1661 Fragments arranged by size: 1661 124 30 SmaI CCC'GGG Cuts at: 0 349 1815 Size: 349 1466 SmlI C'TyrA_G Cuts at: 0 1370 1662 1815 Size: 1370 292 153 Fragments arranged by size: 1370 292 153 SpeI A'CTAG_T Cuts at: 0 1102 1815 Size: 1102 713 StyI C'CwwG_G Cuts at: 0 665 1276 1282 1383 1815 Size: 665 611 6 101 432 Fragments arranged by size: 665 611 432 101 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1006 1815 Size: 1006 809 TatI w'GTAC_w Cuts at: 0 11 56 1815 Size: 11 45 1759 Fragments arranged by size: 1759 45 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 343 375 1815 Size: 343 32 1440 Fragments arranged by size: 1440 343 32 Tth111I GACn'n_nGTC Cuts at: 0 619 1815 Size: 619 1196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1765 1815 Size: 1765 50 XhoI C'TCGA_G Cuts at: 0 1370 1815 Size: 1370 445 XmaI C'CCGG_G Cuts at: 0 347 1815 Size: 347 1468 XmnI GAAnn'nnTTC Cuts at: 0 385 1815 Size: 385 1430 Enzymes that do cut: AcuI AflII AleI ApoI AvaI BamHI BanI BanII BbsI BciVI BglI BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstYI BtgI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NruI PfoI PvuII SacII SmaI SmlI SpeI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NarI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI XbaI ZraI