################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 558 TCCGGA T^CCGGA Alw44I 1261 GTGCAC G^TGCAC AocI 848 CCTCAGG CC^T[ACGT]AGG ApaLI 1261 GTGCAC G^TGCAC AspI 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 406 TGCGCA TGC^GCA AvrII 117 CCTAGG C^CTAGG BanI 532 GGTGCC G^G[CT][GA]CC BcnI 141 CCGGG CC^[GC]GG BlnI 117 CCTAGG C^CTAGG BsaI 934 GAGACC GGTCTC(1/5) BseAI 558 TCCGGA T^CCGGA BsmAI 934 GAGAC GTCTC(1/5) BspEI 558 TCCGGA T^CCGGA BspMI 950 ACCTGC ACCTGC(4/8) BssHII 375 GCGCGC G^CGCGC Bsu36I 848 CCTCAGG CC^T[ACGT]AGG DraIII 592 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 848 CCTCAGG CC^T[ACGT]AGG EcoNI 1158 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 117 CCTAGG C^C[AT][AT]GG FspI 406 TGCGCA TGC^GCA MaeII 826 ACGT A^CGT MroI 558 TCCGGA T^CCGGA MseI 812 TTAA T^TAA NciI 141 CCGGG CC^[GC]GG PaeR7I 69 CTCGAG C^TCGAG PflFI 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1232 GGGACCC [GA]G^G[AT]CC[CT] SfcI 791 CTGTAG C^T[GA][CT]AG StuI 311 AGGCCT AGG^CCT StyI 117 CCTAGG C^C[AT][AT]GG Tru9I 812 TTAA T^TAA Tsp45I 166 GTCAC ^GT[GC]AC Tsp509I 1050 AATT ^AATT Tth111I 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1028 CCAGCGCTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 69 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 306 GTAC GT^AC 1177 GTAC AlwI 495 GATCC GGATC(4/5) 698 GATCC Aor51HI 1030 AGCGCT AGC^GCT 1220 AGCGCT AvaI 69 CTCGAG C^[CT]CG[GA]G 1095 CTCGGG AvaII 600 GGACC G^G[AT]CC 1233 GGACC BanII 72 GAGCCC G[GA]GC[CT]^C 261 GGGCTC BbsI 646 GAAGAC GAAGAC(2/6) 946 GAAGAC BpuAI 646 GAAGAC GAAGAC(2/6) 946 GAAGAC BsaWI 529 TCCGGT [AT]^CCGG[AT] 558 TCCGGA BsoBI 69 CTCGAG C^[CT]CG[GA]G 1095 CTCGGG BstXI 33 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1028 CCAGCGCTCTGG BstYI 494 AGATCC [GA]^GATC[CT] 697 AGATCC DraII 638 AGGCCCT [GA]G^G[ACGT]CC[CT] 1232 GGGACCC DsaI 536 CCGTGG C^C[GA][CT]GG 596 CCGTGG EarI 985 GAAGAG CTCTTC(1/4) 1138 GAAGAG Eco47III 1030 AGCGCT AGC^GCT 1220 AGCGCT EcoO109I 638 AGGCCCT [GA]G^G[ACGT]CC[CT] 1232 GGGACCC FauI 353 CCCGC CCCGC(4/6) 1246 CCCGC HinfI 991 GAGTC G^A[ACGT]TC 1147 GAGTC Ksp632I 985 GAAGAG CTCTTC(1/4) 1138 GAAGAG MaeIII 166 GTCAC ^GT[ACGT]AC 1091 GTAAC MflI 494 AGATCC [GA]^GATC[CT] 697 AGATCC NspI 50 ACATGC [GA]CATG^[CT] 200 ACATGC PleI 991 GAGTC GAGTC(4/5) 1147 GAGTC RsaI 306 GTAC GT^AC 1177 GTAC SmlI 69 CTCGAG C^T[CT][GA]AG 724 CTCAAG XhoII 494 AGATCC [GA]^GATC[CT] 697 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AluI AspHI BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I