(Circular) (Six-base) MAPSORT of: pFN21AE1422.seq Check: 950 from: 1 to: 5980 pFN21AE1422 5980 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 4043 5427 5695 1632 Size: 1100 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1100 268 AfeI AGC'GCT Cuts at: 3037 3227 3037 Size: 190 5790 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 3723 5295 3723 Size: 1572 4408 ApaLI G'TGCA_C Cuts at: 3266 4037 5193 3266 Size: 771 1156 4053 Fragments arranged by size: 4053 1156 771 ApoI r'AATT_y Cuts at: 1493 3289 3515 3667 5776 1493 Size: 1796 226 152 2109 1697 Fragments arranged by size: 2109 1796 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2074 3100 3305 1870 Size: 73 131 1026 205 4545 Fragments arranged by size: 4545 1026 205 131 73 AvrII C'CTAG_G Cuts at: 2122 2122 Size: 5980 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 3301 4756 618 Size: 325 217 627 750 764 1455 1842 Fragments arranged by size: 1842 1455 764 750 627 325 217 BanII G_rGCy'C Cuts at: 1391 2081 2270 3299 1391 Size: 690 189 1029 4072 Fragments arranged by size: 4072 1029 690 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2658 2958 928 Size: 971 759 300 3950 Fragments arranged by size: 3950 971 759 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 3270 4041 5197 1790 Size: 1480 771 1156 2573 Fragments arranged by size: 2573 1480 1156 771 BmgBI CAC'GTC Cuts at: 1318 2832 1318 Size: 1514 4466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2450 2603 3070 3245 4640 1239 Size: 1130 81 153 467 175 1395 2579 Fragments arranged by size: 2579 1395 1130 467 175 153 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 4105 4970 5268 2714 Size: 1391 865 298 3426 Fragments arranged by size: 3426 1391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2933 4643 882 Size: 617 1434 1710 2219 Fragments arranged by size: 2219 1710 1434 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2534 2563 4413 4811 5085 5232 5643 2534 Size: 29 1850 398 274 147 411 2871 Fragments arranged by size: 2871 1850 411 398 274 147 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2303 2333 3260 3290 1178 Size: 30 165 30 900 30 927 30 3868 Fragments arranged by size: 3868 927 900 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2441 2570 2602 2654 2818 3008 3077 Size: 964 291 129 32 52 164 190 69 Cuts at: 3077 3161 3164 3230 1186 Size: 84 3 66 3936 Fragments arranged by size: 3936 964 291 190 164 129 84 69 66 52 32 3 BseYI C'CCAG_C Cuts at: 2081 2111 2445 2776 5183 5679 2081 Size: 30 334 331 2407 496 2382 Fragments arranged by size: 2407 2382 496 334 331 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2004 2240 2579 3266 1163 Size: 573 268 236 339 687 3877 Fragments arranged by size: 3877 687 573 339 268 236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2478 3261 3270 4041 4126 5197 2181 Size: 297 783 9 771 85 1071 2964 Fragments arranged by size: 2964 1071 783 771 297 85 9 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2181 2270 2478 3261 3270 3299 Size: 399 291 100 89 208 783 9 29 Cuts at: 3299 4041 4126 5197 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 291 208 100 89 85 29 9 BspEI T'CCGG_A Cuts at: 2563 5643 2563 Size: 3080 2900 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2964 3335 844 Size: 2120 371 3489 Fragments arranged by size: 3489 2120 371 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 2380 1925 Size: 455 5525 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2045 3040 2045 Size: 995 4985 BstYI r'GATC_y Cuts at: 1972 2499 2702 3310 3677 4062 4079 5531 5617 Size: 527 203 608 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 608 527 385 367 346 203 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2854 2854 Size: 5980 BtgI C'CryG_G Cuts at: 513 1065 2004 2541 2601 513 Size: 552 939 537 60 3892 Fragments arranged by size: 3892 939 552 537 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 2414 2971 3496 4290 4310 2414 Size: 557 525 794 20 4084 Fragments arranged by size: 4084 794 557 525 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DraIII CAC_nnn'GTG Cuts at: 2602 2602 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945 Size: 54 1425 50 275 18 1597 889 1629 Cuts at: 5945 8 Size: 43 Fragments arranged by size: 1629 1597 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2985 3138 3910 1353 Size: 1632 153 772 3423 Fragments arranged by size: 3423 1632 772 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2914 3236 4567 4941 5087 387 Size: 1064 138 1325 322 1331 374 146 1280 Fragments arranged by size: 1331 1325 1280 1064 374 322 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2450 2603 2732 3070 3245 4043 Size: 393 737 81 153 129 338 175 798 Cuts at: 4043 4640 5427 5695 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 798 787 737 597 393 338 268 175 153 129 81 EcoNI CCTnn'n_nnAGG Cuts at: 3167 3167 Size: 5980 EcoO109I rG'GnC_Cy Cuts at: 2644 3238 2644 Size: 594 5386 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 2413 4486 2413 Size: 2073 3907 FspAI rTGC'GCAy Cuts at: 2413 2413 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2373 3039 3229 5127 1678 Size: 695 666 190 1898 2531 Fragments arranged by size: 2531 1898 695 666 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 2930 2962 3137 Size: 32 135 32 1025 32 301 32 175 Cuts at: 3137 3169 4621 4653 4695 4727 1373 Size: 32 1452 32 42 32 2626 Fragments arranged by size: 2626 1452 1025 301 175 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2479 5094 5278 1305 Size: 1174 2615 184 2007 Fragments arranged by size: 2615 2007 1174 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3938 4297 4456 518 Size: 648 2772 359 159 2042 Fragments arranged by size: 2772 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3396 4073 5221 5466 1606 Size: 1790 677 1148 245 2120 Fragments arranged by size: 2120 1790 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2059 2209 3338 4883 1132 Size: 97 830 150 1129 1545 2229 Fragments arranged by size: 2229 1545 1129 830 150 97 PasI CC'CwG_GG Cuts at: 1348 2045 1348 Size: 697 5283 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2145 2187 2484 2145 Size: 42 297 5641 Fragments arranged by size: 5641 297 42 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3238 3238 Size: 5980 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspXI vC'TCGA_Gb Cuts at: 2074 2074 Size: 5980 PstI C_TGCA'G Cuts at: 830 3332 830 Size: 2502 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SanDI GG'GwC_CC Cuts at: 3238 3238 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2796 3328 4463 5144 5335 826 Size: 219 1751 532 1135 681 191 1471 Fragments arranged by size: 1751 1471 1135 681 532 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 2729 4084 4985 5247 820 Size: 197 926 131 655 1355 901 262 1553 Fragments arranged by size: 1553 1355 926 901 655 262 197 131 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 2318 1517 Size: 801 5179 StyI C'CwwG_G Cuts at: 513 1065 2004 2122 3419 513 Size: 552 939 118 1297 3074 Fragments arranged by size: 3074 1297 939 552 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 3260 3292 5940 5972 2393 Size: 32 835 32 2648 32 2401 Fragments arranged by size: 2648 2401 835 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2944 1316 Size: 1628 4352 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3040 3040 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 2074 1943 Size: 131 5849 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI