(Linear) (Six-base) MAPSORT of: KIEE1422.seq Check: 5926 from: 1 to: 1275 KIEE1422.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 1275 Size: 727 548 AfeI AGC'GCT Cuts at: 0 1032 1222 1275 Size: 1032 190 53 Fragments arranged by size: 1032 190 53 ApaLI G'TGCA_C Cuts at: 0 1261 1275 Size: 1261 14 AvaI C'yCGr_G Cuts at: 0 69 1095 1275 Size: 69 1026 180 Fragments arranged by size: 1026 180 69 AvrII C'CTAG_G Cuts at: 0 117 1275 Size: 117 1158 BanI G'GyrC_C Cuts at: 0 532 1275 Size: 532 743 BanII G_rGCy'C Cuts at: 0 76 265 1275 Size: 76 189 1010 Fragments arranged by size: 1010 189 76 BbsI GAAGACnn'nnnn_ Cuts at: 0 653 953 1275 Size: 653 300 322 Fragments arranged by size: 653 322 300 Bme1580I G_kGCm'C Cuts at: 0 1265 1275 Size: 1265 10 BmgBI CAC'GTC Cuts at: 0 827 1275 Size: 827 448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 445 598 1065 1240 1275 Size: 364 81 153 467 175 35 Fragments arranged by size: 467 364 175 153 81 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 1275 Size: 709 566 BsaI GGTCTCn'nnnn_ Cuts at: 0 928 1275 Size: 928 347 BsaWI w'CCGG_w Cuts at: 0 529 558 1275 Size: 529 29 717 Fragments arranged by size: 717 529 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 298 328 1255 1275 Size: 298 30 927 20 Fragments arranged by size: 927 298 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 145 436 565 597 649 813 1003 1072 Size: 145 291 129 32 52 164 190 69 Cuts at: 1072 1156 1159 1225 1275 Size: 84 3 66 50 Fragments arranged by size: 291 190 164 145 129 84 69 66 52 50 32 3 BseYI C'CCAG_C Cuts at: 0 76 106 440 771 1275 Size: 76 30 334 331 504 Fragments arranged by size: 504 334 331 76 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 574 1261 1275 Size: 235 339 687 14 Fragments arranged by size: 687 339 235 14 BsiHKAI G_wGCw'C Cuts at: 0 176 473 1256 1265 1275 Size: 176 297 783 9 10 Fragments arranged by size: 783 297 176 10 9 Bsp1286I G_dGCh'C Cuts at: 0 76 176 265 473 1256 1265 1275 Size: 76 100 89 208 783 9 10 Fragments arranged by size: 783 208 100 89 76 10 9 BspEI T'CCGG_A Cuts at: 0 558 1275 Size: 558 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 959 1275 Size: 959 316 BssHII G'CGCG_C Cuts at: 0 375 1275 Size: 375 900 BstXI CCAn_nnnn'nTGG Cuts at: 0 40 1035 1275 Size: 40 995 240 Fragments arranged by size: 995 240 40 BstYI r'GATC_y Cuts at: 0 494 697 1275 Size: 494 203 578 Fragments arranged by size: 578 494 203 Bsu36I CC'TnA_GG Cuts at: 0 849 1275 Size: 849 426 BtgI C'CryG_G Cuts at: 0 536 596 1275 Size: 536 60 679 Fragments arranged by size: 679 536 60 BtsI GCAGTG_nn' Cuts at: 0 409 966 1275 Size: 409 557 309 Fragments arranged by size: 557 409 309 DraIII CAC_nnn'GTG Cuts at: 0 597 1275 Size: 597 678 EarI CTCTTCn'nnn_ Cuts at: 0 980 1133 1275 Size: 980 153 142 Fragments arranged by size: 980 153 142 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 909 1231 1275 Size: 909 322 44 Fragments arranged by size: 909 322 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 445 598 727 1065 1240 1275 Size: 364 81 153 129 338 175 35 Fragments arranged by size: 364 338 175 153 129 81 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 1162 1275 Size: 1162 113 EcoO109I rG'GnC_Cy Cuts at: 0 639 1233 1275 Size: 639 594 42 Fragments arranged by size: 639 594 42 FspI TGC'GCA Cuts at: 0 408 1275 Size: 408 867 FspAI rTGC'GCAy Cuts at: 0 408 1275 Size: 408 867 HaeII r_GCGC'y Cuts at: 0 368 1034 1224 1275 Size: 368 666 190 51 Fragments arranged by size: 666 368 190 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 592 624 925 957 1132 1164 1275 Size: 592 32 301 32 175 32 111 Fragments arranged by size: 592 301 175 111 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 474 1275 Size: 474 801 NspI r_CATG'y Cuts at: 0 54 204 1275 Size: 54 150 1071 Fragments arranged by size: 1071 150 54 PasI CC'CwG_GG Cuts at: 0 40 1275 Size: 40 1235 PfoI T'CCnGG_A Cuts at: 0 140 182 479 1275 Size: 140 42 297 796 Fragments arranged by size: 796 297 140 42 PpuMI rG'GwC_Cy Cuts at: 0 1233 1275 Size: 1233 42 PspXI vC'TCGA_Gb Cuts at: 0 69 1275 Size: 69 1206 SanDI GG'GwC_CC Cuts at: 0 1233 1275 Size: 1233 42 SfcI C'TryA_G Cuts at: 0 791 1275 Size: 791 484 SmlI C'TyrA_G Cuts at: 0 69 724 1275 Size: 69 655 551 Fragments arranged by size: 655 551 69 StuI AGG'CCT Cuts at: 0 313 1275 Size: 313 962 StyI C'CwwG_G Cuts at: 0 117 1275 Size: 117 1158 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 388 420 1255 1275 Size: 388 32 835 20 Fragments arranged by size: 835 388 32 20 Tth111I GACn'n_nGTC Cuts at: 0 939 1275 Size: 939 336 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1035 1275 Size: 1035 240 XhoI C'TCGA_G Cuts at: 0 69 1275 Size: 69 1206 Enzymes that do cut: AcuI AfeI ApaLI AvaI AvrII BanI BanII BbsI Bme1580I BmgBI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BssHII BstXI BstYI Bsu36I BtgI BtsI DraIII EarI EciI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I MmeI NspI PasI PfoI PpuMI PspXI SanDI SfcI SmlI StuI StyI TstI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI XmaI XmnI ZraI