################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NdeI NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XcmI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwNI 118 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 228 CCTGAGG CC^T[ACGT]AGG ApaI 867 GGGCCC GGGCC^C Asp700I 837 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 126 GGACC G^G[AT]CC BanII 867 GGGCCC G[GA]GC[CT]^C BciVI 209 GTATCC GTATCC(6/5) BclI 680 TGATCA T^GATCA BmyI 867 GGGCCC G[AGT]GC[ACT]^C BsaBI 504 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 140 ACCGGA [AT]^CCGG[AT] BsmAI 926 GTCTC GTCTC(1/5) BsmBI 925 CGTCTC CGTCTC(1/5) Bsp1286I 867 GGGCCC G[AGT]GC[ACT]^C BspMI 385 GCAGGT ACCTGC(4/8) BsrBI 153 GAGCGG CCGCTC(-3/-3) BsrDI 723 CATTGC GCAATG(2/0) BstBI 506 TTCGAA TT^CGAA Bsu36I 228 CCTGAGG CC^T[ACGT]AGG DpnII 681 GATC ^GATC DraIII 534 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 228 CCTGAGG CC^T[ACGT]AGG EcoNI 831 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 680 TGATCA T^GATCA MaeII 791 ACGT A^CGT MaeIII 694 GTCAC ^GT[ACGT]AC MamI 504 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 681 GATC ^GATC MseI 494 TTAA T^TAA NcoI 560 CCATGG C^CATGG NdeII 681 GATC ^GATC NspI 746 ACATGC [GA]CATG^[CT] NspV 506 TTCGAA TT^CGAA PaeR7I 173 CTCGAG C^TCGAG PflMI 117 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 461 CAGCTG CAG^CTG Sau3AI 681 GATC ^GATC SexAI 128 ACCTGGT A^CC[AT]GGT SfcI 104 CTACAG C^T[GA][CT]AG SfuI 506 TTCGAA TT^CGAA SmaI 515 CCCGGG CCC^GGG SmlI 173 CTCGAG C^T[CT][GA]AG Tru9I 494 TTAA T^TAA Tsp45I 694 GTCAC ^GT[GC]AC Van91I 117 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 173 CTCGAG C^TCGAG XmaCI 515 CCCGGG C^CCGGG XmaI 515 CCCGGG C^CCGGG XmnI 837 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BanI 388 GGTGCC G^G[CT][GA]CC 397 GGTGCC BglI 227 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 418 GCCGAAGTGGC BsrFI 25 GCCGGC [GA]^CCGG[CT] 452 GCCGGC BstXI 468 CCAGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 710 CCATGCCTGTGG CfoI 321 GCGC GCG^C 471 GCGC Cfr10I 25 GCCGGC [GA]^CCGG[CT] 452 GCCGGC DraII 83 GGGGCCT [GA]G^G[ACGT]CC[CT] 867 GGGCCCC DsaI 474 CCGTGG C^C[GA][CT]GG 560 CCATGG EaeI 89 TGGCCG [CT]^GGCC[GA] 450 TGGCCG EarI 928 CTCTTC CTCTTC(1/4) 520 GAAGAG EcoO109I 83 GGGGCCT [GA]G^G[ACGT]CC[CT] 867 GGGCCCC EcoT14I 294 CCTTGG C^C[AT][AT]GG 560 CCATGG HaeII 320 AGCGCC [GA]GCGC^[CT] 470 AGCGCC HgaI 267 GCGTC GACGC(5/10) 924 GCGTC HhaI 321 GCGC GCG^C 471 GCGC HinP1I 321 GCGC G^CGC 471 GCGC Ksp632I 928 CTCTTC CTCTTC(1/4) 520 GAAGAG MspA1I 461 CAGCTG C[AC]G^C[GT]G 684 CAGCGG NaeI 25 GCCGGC GCC^GGC 452 GCCGGC NgoMIV 25 GCCGGC G^CCGGC 452 GCCGGC PleI 171 GACTC GAGTC(4/5) 631 GACTC StuI 225 AGGCCT AGG^CCT 914 AGGCCT StyI 294 CCTTGG C^C[AT][AT]GG 560 CCATGG TaqI 174 TCGA T^CGA 507 TCGA TthHB8I 174 TCGA T^CGA 507 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AvaI BbvI BcnI BsaJI BsiYI BslI BsoBI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TfiI TseI TspRI