Restriction map for
pFN21AE1514
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 238 AAATTT [GA]^AATT[CT]
AlwI 262 GATCC GGATC(4/5)
ApoI 238 AAATTT [GA]^AATT[CT]
BanI 699 GGTGCC G^G[CT][GA]CC
BanII 523 GAGCTC G[GA]GC[CT]^C
BbsI 424 GAAGAC GAAGAC(2/6)
BcnI 653 CCCGG CC^[GC]GG
BfaI 688 CTAG C^TAG
BpuAI 424 GAAGAC GAAGAC(2/6)
BsaI 105 GGTCTC GGTCTC(1/5)
BsmAI 106 GTCTC GTCTC(1/5)
BsmFI 500 GGGAC GGGAC(10/14)
BsmI 74 GCATTC GAATGC(1/-1)
Bsp1407I 281 TGTACA T^GTACA
BspHI 323 TCATGA T^CATGA
BsrGI 281 TGTACA T^GTACA
BsrI 343 ACTGG ACTGG(1/-1)
BstAPI 620 GCACGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 66 GGTGACC G^GT[ACGT]ACC
BstF5I 432 GGATG GGATG(2/0)
BstPI 66 GGTGACC G^GT[ACGT]ACC
Cac8I 143 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 275 GCGC GCG^C
DraI 241 TTTAAA TTT^AAA
DraII 508 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 101 CCGTGG C^C[GA][CT]GG
EcoO109I 508 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
FokI 432 GGATG GGATG(9/13)
HhaI 275 GCGC GCG^C
HinP1I 275 GCGC G^CGC
HphI 66 GGTGA GGTGA(8/7)
MaeI 688 CTAG C^TAG
MaeII 119 ACGT A^CGT
MseI 242 TTAA T^TAA
NciI 653 CCCGG CC^[GC]GG
NdeI 94 CATATG CA^TATG
NlaIV 699 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 8 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 99 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 323 TCATGA T^CATGA
SacI 523 GAGCTC GAGCT^C
ScaI 224 AGTACT AGT^ACT
SspBI 281 TGTACA T^GTACA
TaqI 185 TCGA T^CGA
Tru9I 242 TTAA T^TAA
TthHB8I 185 TCGA T^CGA
Van91I 8 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 371 AGCT AG^CT
524 AGCT
AspHI 3 GTGCTC G[AT]GC[AT]^C
523 GAGCTC
AvaII 294 GGTCC G^G[AT]CC
656 GGTCC
BsaJI 101 CCGTGG C^C[ACGT][ACGT]GG
647 CCCTGG
BsiHKAI 3 GTGCTC G[AT]GC[AT]^C
523 GAGCTC
DpnII 262 GATC ^GATC
366 GATC
HapII 110 CCGG C^CGG
654 CCGG
HpaII 110 CCGG C^CGG
654 CCGG
MboI 262 GATC ^GATC
366 GATC
MslI 324 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
549 CACCCTTATG
MspI 110 CCGG C^CGG
654 CCGG
NdeII 262 GATC ^GATC
366 GATC
PleI 123 GACTC GAGTC(4/5)
475 GAGTC
Sau3AI 262 GATC ^GATC
366 GATC
SexAI 169 ACCTGGT A^CC[AT]GGT
490 ACCTGGT
TspRI 278 CACTG CA[GC]TG[ACGT][ACGT]^
534 CAGTG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI BbvI BmyI BpmI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HinfI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIII RsaI Sau96I ScrFI
TfiI TseI Tsp45I Tsp509I