(Linear) (Six-base) MAPSORT of: KIEE1514.seq  Check: 203  from: 1  to: 729



KIEE1514.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

ApoI r'AATT_y

Cuts at:      0     238     729 
   Size:        238     491
 
BanI G'GyrC_C

Cuts at:      0     699     729 
   Size:        699      30
 
BanII G_rGCy'C

Cuts at:      0     527     729 
   Size:        527     202
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     431     729 
   Size:        431     298
 
Bme1580I G_kGCm'C

Cuts at:      0     704     729 
   Size:        704      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     353     729 
   Size:        353     376
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     555     729 
   Size:        511      44     174

  Fragments arranged by size: 

                 511     174      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     111     729 
   Size:        111     618
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     527     729 
   Size:          7     520     202

  Fragments arranged by size: 

                 520     202       7
 
BsmI GAATG_Cn'

Cuts at:      0      74     729 
   Size:         74     655
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     527     704     729 
   Size:          7     520     177      25

  Fragments arranged by size: 

                 520     177      25       7
 
BspHI T'CATG_A

Cuts at:      0     323     729 
   Size:        323     406
 
BsrGI T'GTAC_A

Cuts at:      0     281     729 
   Size:        281     448
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     626     729 
   Size:        626     103
 
BstEII G'GTnAC_C

Cuts at:      0      66     729 
   Size:         66     663
 
BtgI C'CryG_G

Cuts at:      0     101     729 
   Size:        101     628
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     693     728     729 
   Size:        693      35       1

  Fragments arranged by size: 

                 693      35       1
 
DraI TTT'AAA

Cuts at:      0     243     729 
   Size:        243     486
 
EcoICRI GAG'CTC

Cuts at:      0     525     729 
   Size:        525     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     555     729 
   Size:        511      44     174

  Fragments arranged by size: 

                 511     174      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     509     729 
   Size:        509     220
 
MslI CAynn'nnrTG

Cuts at:      0     328     553     729 
   Size:        328     225     176

  Fragments arranged by size: 

                 328     225     176
 
NdeI CA'TA_TG

Cuts at:      0      95     729 
   Size:         95     634
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      14     729 
   Size:         14     715
 
PshAI GACnn'nnGTC

Cuts at:      0     103     729 
   Size:        103     626
 
SacI G_AGCT'C

Cuts at:      0     527     729 
   Size:        527     202
 
ScaI AGT'ACT

Cuts at:      0     226     729 
   Size:        226     503
 
SexAI A'CCwGG_T

Cuts at:      0     169     490     729 
   Size:        169     321     239

  Fragments arranged by size: 

                 321     239     169
 
TatI w'GTAC_w

Cuts at:      0     224     281     729 
   Size:        224      57     448

  Fragments arranged by size: 

                 448     224      57
 

 Enzymes that do cut:

     ApoI     BanI    BanII     BbsI Bme1580I     BmrI     BpmI     BsaI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrGI   BstAPI   BstEII     BtgI
    CspCI     DraI  EcoICRI  Eco57MI EcoO109I     MslI     NdeI    PflMI
    PshAI     SacI     ScaI    SexAI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI