Restriction map for
pFN21AE3620
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AluI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII BamHI
BanII BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsgI
BsiEI BsiWI BsmFI BsmI BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MboII MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 448 GACGTC GACGT^C
AccIII 583 TCCGGA T^CCGGA
AcyI 448 GACGTC G[GA]^CG[CT]C
AlwNI 497 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 568 GTGCTC G[AT]GC[AT]^C
AvrII 271 CCTAGG C^CTAGG
BalI 243 TGGCCA TGG^CCA
BanI 55 GGCACC G^G[CT][GA]CC
BbvI 502 GCTGC GCAGC(8/12)
BcgI 492 CGAAGCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 40 CCCGG CC^[GC]GG
BlnI 271 CCTAGG C^CTAGG
BsaHI 448 GACGTC G[GA]^CG[CT]C
BsaI 88 GGTCTC GGTCTC(1/5)
BsaWI 583 TCCGGA [AT]^CCGG[AT]
BseAI 583 TCCGGA T^CCGGA
BseRI 563 GAGGAG GAGGAG(10/8)
BsiHKAI 568 GTGCTC G[AT]GC[AT]^C
Bsp1407I 432 TGTACA T^GTACA
BspEI 583 TCCGGA T^CCGGA
BsrGI 432 TGTACA T^GTACA
BssHII 18 GCGCGC G^CGCGC
BstXI 78 CCAGAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 318 GGATCT [GA]^GATC[CT]
DraIII 57 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 271 CCTAGG C^C[AT][AT]GG
FauI 120 GCGGG CCCGC(4/6)
Hin1I 448 GACGTC G[GA]^CG[CT]C
HinfI 381 GATTC G^A[ACGT]TC
MaeIII 451 GTCAC ^GT[ACGT]AC
MflI 318 GGATCT [GA]^GATC[CT]
MluNI 243 TGGCCA TGG^CCA
MroI 583 TCCGGA T^CCGGA
MscI 243 TGGCCA TGG^CCA
MseI 356 TTAA T^TAA
MslI 430 CATGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 105 CAGCGG C[AC]G^C[GT]G
NciI 40 CCCGG CC^[GC]GG
SfaNI 96 GCATC GCATC(5/9)
SspBI 432 TGTACA T^GTACA
SspI 337 AATATT AAT^ATT
StyI 271 CCTAGG C^C[AT][AT]GG
TfiI 381 GATTC G^A[AT]TC
Tru9I 356 TTAA T^TAA
TseI 502 GCTGC G^C[AT]GC
Tsp45I 451 GTCAC ^GT[GC]AC
XhoII 318 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 19 CGCG CG^CG
21 CGCG
AfaI 433 GTAC GT^AC
519 GTAC
AlwI 71 GGATC GGATC(4/5)
318 GGATC
AvaI 31 CTCGGG C^[CT]CG[GA]G
45 CTCGGG
BclI 203 TGATCA T^GATCA
326 TGATCA
BmyI 54 GGGCAC G[AGT]GC[ACT]^C
568 GTGCTC
BpmI 91 CTCCAG CTGGAG(16/14)
102 CTCCAG
BsmBI 364 CGTCTC CGTCTC(1/5)
592 GAGACG
BsoBI 31 CTCGGG C^[CT]CG[GA]G
45 CTCGGG
Bsp1286I 54 GGGCAC G[AGT]GC[ACT]^C
568 GTGCTC
BstAPI 56 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
496 GCAGTTGCTGC
BstF5I 27 CATCC GGATG(2/0)
480 GGATG
BstUI 19 CGCG CG^CG
21 CGCG
CfoI 18 GCGC GCG^C
20 GCGC
Cfr13I 34 GGGCC G^G[ACGT]CC
608 GGCCC
DdeI 367 CTCAG C^T[ACGT]AG
511 CTAAG
EaeI 108 CGGCCA [CT]^GGCC[GA]
243 TGGCCA
FbaI 203 TGATCA T^GATCA
326 TGATCA
FokI 27 CATCC GGATG(9/13)
480 GGATG
HapII 41 CCGG C^CGG
584 CCGG
HhaI 18 GCGC GCG^C
20 GCGC
HinP1I 18 GCGC G^CGC
20 GCGC
HpaII 41 CCGG C^CGG
584 CCGG
MspI 41 CCGG C^CGG
584 CCGG
MvnI 19 CGCG CG^CG
21 CGCG
RsaI 433 GTAC GT^AC
519 GTAC
Sau96I 34 GGGCC G^G[ACGT]CC
608 GGCCC
Tsp509I 548 AATT ^AATT
578 AATT
TspRI 250 CACTG CA[GC]TG[ACGT][ACGT]^
453 CACTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BfaI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI Cac8I
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MaeI MaeII MboI MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI