################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### BglI 1091 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 105 GTGCAG GTGCAG(16/14) BsmI 714 GAATGC GAATGC(1/-1) BsrBI 781 GAGCGG CCGCTC(-3/-3) BsrDI 237 GCAATG GCAATG(2/0) BstXI 606 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 318 GATC ^GATC DraIII 79 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1054 CTCTTC CTCTTC(1/4) EcoRV 9 GATATC GAT^ATC FauI 783 GCGGG CCCGC(4/6) Ksp632I 1054 CTCTTC CTCTTC(1/4) MboI 318 GATC ^GATC MspA1I 915 CAGCTG C[AC]G^C[GT]G NcoI 953 CCATGG C^CATGG NdeI 1172 CATATG CA^TATG NdeII 318 GATC ^GATC PvuII 915 CAGCTG CAG^CTG Sau3AI 318 GATC ^GATC SfcI 131 CTGTAG C^T[GA][CT]AG StuI 680 AGGCCT AGG^CCT Tsp509I 1168 AATT ^AATT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 181 CGCG CG^CG 252 CGCG AocI 491 CCTCAGG CC^T[ACGT]AGG 925 CCTAAGG ApaI 446 GGGCCC GGGCC^C 616 GGGCCC BalI 35 TGGCCA TGG^CCA 1070 TGGCCA BanI 301 GGCACC G^G[CT][GA]CC 1182 GGTGCC BbrPI 102 CACGTG CAC^GTG 585 CACGTG BbvCI 631 GCTGAGG CCTCAGC(-5/-2) 931 GCTGAGG BsaAI 102 CACGTG [CT]AC^GT[GA] 585 CACGTG BstUI 181 CGCG CG^CG 252 CGCG Bsu36I 491 CCTCAGG CC^T[ACGT]AGG 925 CCTAAGG CfoI 182 GCGC GCG^C 808 GCGC DsaI 78 CCACGG C^C[GA][CT]GG 953 CCATGG EaeI 35 TGGCCA [CT]^GGCC[GA] 1070 TGGCCA Eco81I 491 CCTCAGG CC^T[ACGT]AGG 925 CCTAAGG EcoT14I 953 CCATGG C^C[AT][AT]GG 1044 CCTTGG HhaI 182 GCGC GCG^C 808 GCGC HinP1I 182 GCGC G^CGC 808 GCGC HinfI 367 GAGTC G^A[ACGT]TC 420 GACTC HphI 327 GGTGA GGTGA(8/7) 1079 TCACC MaeIII 85 GTGAC ^GT[ACGT]AC 481 GTGAC MboII 427 TCTTC GAAGA(8/7) 1055 TCTTC MluNI 35 TGGCCA TGG^CCA 1070 TGGCCA MscI 35 TGGCCA TGG^CCA 1070 TGGCCA MslI 76 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 811 CACACGGATG MvnI 181 CGCG CG^CG 252 CGCG PflMI 620 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 963 CCAGAGGCTGG PleI 420 GACTC GAGTC(4/5) 367 GAGTC PmaCI 102 CACGTG CAC^GTG 585 CACGTG PmlI 102 CACGTG CAC^GTG 585 CACGTG StyI 953 CCATGG C^C[AT][AT]GG 1044 CCTTGG Tsp45I 85 GTGAC ^GT[GC]AC 481 GTGAC Van91I 620 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 963 CCAGAGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcyI AluI AlwNI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI Hin1I HpaII ItaI MaeII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I