(Circular) (Six-base) MAPSORT of: pFN21AE1310.seq Check: 1 from: 1 to: 5896 pFN21AE1310 5896 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3239 1937 Size: 1302 4594 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 2898 3959 5343 5611 1632 Size: 1119 147 1061 1384 268 1917 Fragments arranged by size: 1917 1384 1119 1061 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 2085 1166 Size: 919 4977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2619 2838 1184 Size: 1435 219 4242 Fragments arranged by size: 4242 1435 219 AlwNI CAG_nnn'CTG Cuts at: 2269 2631 2974 3639 5211 2269 Size: 362 343 665 1572 2954 Fragments arranged by size: 2954 1572 665 362 343 ApaI G_GGCC'C Cuts at: 2455 2625 2455 Size: 170 5726 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 3205 3431 3583 5692 1493 Size: 1712 226 152 2109 1697 Fragments arranged by size: 2109 1712 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2306 3187 3217 4672 618 Size: 325 217 627 519 881 30 1455 1842 Fragments arranged by size: 1842 1455 881 627 519 325 217 30 BanII G_rGCy'C Cuts at: 1391 2391 2455 2625 2765 3215 1391 Size: 1000 64 170 140 450 4072 Fragments arranged by size: 4072 1000 450 170 140 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4925 BbvCI CC'TCA_GC Cuts at: 2637 2937 2637 Size: 300 5596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3102 4507 136 Size: 107 122 71 631 605 1430 1405 1525 Fragments arranged by size: 1525 1430 1405 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 2455 2552 2625 3192 3957 5113 1790 Size: 665 97 73 567 765 1156 2573 Fragments arranged by size: 2573 1156 765 665 567 97 73 BmgBI CAC'GTC Cuts at: 1318 2860 1318 Size: 1542 4354 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2845 3627 4585 Size: 416 53 83 399 24 1428 782 958 Cuts at: 4585 442 Size: 1753 Fragments arranged by size: 1753 1428 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2622 2622 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2438 2804 3083 4556 1239 Size: 1166 33 366 279 1473 2579 Fragments arranged by size: 2579 1473 1166 366 279 33 Bpu10I CC'TnA_GC Cuts at: 2529 2637 2937 2529 Size: 108 300 5488 Fragments arranged by size: 5488 300 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4886 5184 4021 Size: 865 298 4733 Fragments arranged by size: 4733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4559 882 Size: 617 3060 2219 Fragments arranged by size: 3060 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2109 2592 3687 493 Size: 711 905 483 1095 2702 Fragments arranged by size: 2702 1095 905 711 483 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2519 2979 3003 3703 Size: 53 83 186 936 986 460 24 700 Cuts at: 3703 4085 4720 275 Size: 382 635 1451 Fragments arranged by size: 1451 986 936 700 635 460 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 4329 4727 5001 5148 5559 4329 Size: 398 274 147 411 4666 Fragments arranged by size: 4666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2529 2559 3151 3181 1178 Size: 30 165 30 1126 30 592 30 3893 Fragments arranged by size: 3893 1126 592 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 2807 2869 3047 1186 Size: 1543 78 62 178 4035 Fragments arranged by size: 4035 1543 178 78 62 BseYI C'CCAG_C Cuts at: 2624 2704 2894 2974 5099 5595 2624 Size: 80 190 80 2125 496 2925 Fragments arranged by size: 2925 2125 496 190 80 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2131 1163 Size: 573 395 4928 Fragments arranged by size: 4928 573 395 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3957 4042 5113 3957 Size: 85 1071 4740 Fragments arranged by size: 4740 1071 85 BsmI GAATG_Cn' Cuts at: 2725 3407 3500 2725 Size: 682 93 5121 Fragments arranged by size: 5121 682 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2195 2581 2866 2195 Size: 386 285 5225 Fragments arranged by size: 5225 386 285 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2455 2552 2625 2765 3192 3215 Size: 399 601 64 97 73 140 427 23 Cuts at: 3215 3957 4042 5113 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 427 399 140 97 85 73 64 23 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3251 844 Size: 2407 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2788 3783 1394 Size: 429 965 995 3507 Fragments arranged by size: 3507 995 965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2249 4391 4565 65 Size: 1183 1001 2142 174 1396 Fragments arranged by size: 2142 1396 1183 1001 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5896 BstXI CCAn_nnnn'nTGG Cuts at: 2618 2618 Size: 5896 BstYI r'GATC_y Cuts at: 1972 3226 3593 3978 3995 5447 5533 5545 5891 Size: 1254 367 385 17 1452 86 12 346 Cuts at: 5891 1972 Size: 1977 Fragments arranged by size: 1977 1452 1254 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2497 2931 2497 Size: 434 5462 BtgI C'CryG_G Cuts at: 513 1065 2004 2083 2958 3192 513 Size: 552 939 79 875 234 3217 Fragments arranged by size: 3217 939 875 552 234 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2273 487 Size: 683 56 1047 4110 Fragments arranged by size: 4110 1047 683 56 BtsI GCAGTG_nn' Cuts at: 2580 2691 3412 4206 4226 2580 Size: 111 721 794 20 4250 Fragments arranged by size: 4250 794 721 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DraIII CAC_nnn'GTG Cuts at: 2089 2089 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 3075 3343 Size: 54 1425 50 275 18 210 1035 268 Cuts at: 3343 4232 5861 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1035 889 275 268 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3065 3826 1353 Size: 1712 761 3423 Fragments arranged by size: 3423 1712 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3149 4483 4857 5003 387 Size: 1064 138 1560 1334 374 146 1280 Fragments arranged by size: 1560 1334 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2438 2751 2804 2898 3083 3959 Size: 393 773 33 313 53 94 185 876 Cuts at: 3959 4556 5343 5611 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 876 787 773 597 393 313 268 185 94 53 33 EcoO109I rG'GnC_Cy Cuts at: 2451 2452 2460 2540 2656 2671 2801 2840 2889 Size: 1 8 80 116 15 130 39 49 Cuts at: 2889 3139 2451 Size: 250 5208 Fragments arranged by size: 5208 250 130 116 80 49 39 15 8 1 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 EcoRV GAT'ATC Cuts at: 2016 2016 Size: 5896 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2645 2677 4537 Size: 32 135 32 679 32 362 32 1860 Cuts at: 4537 4569 4611 4643 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1860 679 362 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5010 5194 1305 Size: 3705 184 2007 Fragments arranged by size: 3705 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 3077 10 Size: 54 1425 343 210 1035 2829 Fragments arranged by size: 2829 1425 1035 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2820 3854 4213 4372 518 Size: 648 919 735 1034 359 159 2042 Fragments arranged by size: 2042 1034 919 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2922 3312 3989 5137 5382 1606 Size: 1316 390 677 1148 245 2120 Fragments arranged by size: 2120 1316 1148 677 390 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2958 513 Size: 552 939 954 3451 Fragments arranged by size: 3451 954 939 552 NdeI CA'TA_TG Cuts at: 387 3178 387 Size: 2791 3105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 3254 4799 1132 Size: 97 2025 1545 2229 Fragments arranged by size: 2229 2025 1545 97 PasI CC'CwG_GG Cuts at: 1348 2455 1348 Size: 1107 4789 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2631 2974 1108 Size: 1523 343 4030 Fragments arranged by size: 4030 1523 343 PfoI T'CCnGG_A Cuts at: 2382 2829 2382 Size: 447 5449 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PmlI CAC'GTG Cuts at: 2109 2592 2109 Size: 483 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2460 2671 2801 2889 2460 Size: 211 130 88 5467 Fragments arranged by size: 5467 211 130 88 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PspOMI G'GGCC_C Cuts at: 2451 2621 2451 Size: 170 5726 PstI C_TGCA'G Cuts at: 830 3248 830 Size: 2418 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 PvuII CAG'CTG Cuts at: 2922 2922 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SanDI GG'GwC_CC Cuts at: 2801 2889 2801 Size: 88 5808 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 2136 3244 4379 5060 5251 826 Size: 219 1091 1108 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 1091 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4000 4901 5163 820 Size: 197 926 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 3254 1132 Size: 2122 3774 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 2687 1517 Size: 1170 4726 StyI C'CwwG_G Cuts at: 513 1065 2004 2958 3049 3335 513 Size: 552 939 954 91 286 3074 Fragments arranged by size: 3074 954 939 552 286 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 2696 3923 3940 4093 4278 4723 Size: 1255 1101 15 1227 17 153 185 445 Cuts at: 4723 325 Size: 1498 Fragments arranged by size: 1498 1255 1227 1101 445 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4142 5854 96 Size: 274 80 33 51 1443 2165 1712 138 Fragments arranged by size: 2165 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2529 2561 5856 5888 2529 Size: 32 3295 32 2537 Fragments arranged by size: 3295 2537 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI