Restriction map for
pFN21AB6907
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp509I TspRI
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 172 CGCG CG^CG
AcyI 406 GGCGTC G[GA]^CG[CT]C
AhdI 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 111 GATCC GGATC(4/5)
AlwNI 115 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 64 GAGCAC G[AT]GC[AT]^C
AvaII 264 GGACC G^G[AT]CC
BanI 492 GGCACC G^G[CT][GA]CC
BlpI 4 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BsaAI 393 TACGTA [CT]AC^GT[GA]
BsaBI 333 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 406 GGCGTC G[GA]^CG[CT]C
BsaWI 329 ACCGGA [AT]^CCGG[AT]
BsgI 234 GTGCAG GTGCAG(16/14)
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
BstUI 172 CGCG CG^CG
BstXI 203 CCAACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 110 AGATCC [GA]^GATC[CT]
CelII 4 GCTGAGC GC^T[ACGT]AGC
DdeI 5 CTGAG C^T[ACGT]AG
DpnII 111 GATC ^GATC
DrdI 190 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 482 CCGTGG C^C[GA][CT]GG
EaeI 176 TGGCCG [CT]^GGCC[GA]
Eam1105I 562 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 96 GAAGAG CTCTTC(1/4)
EcoT14I 497 CCAAGG C^C[AT][AT]GG
FauI 536 CCCGC CCCGC(4/6)
HgaI 407 GCGTC GACGC(5/10)
Hin1I 406 GGCGTC G[GA]^CG[CT]C
HinfI 582 GAATC G^A[ACGT]TC
HpaI 352 GTTAAC GTT^AAC
Ksp632I 96 GAAGAG CTCTTC(1/4)
MaeIII 214 GTGAC ^GT[ACGT]AC
MamI 333 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 111 GATC ^GATC
MboII 96 GAAGA GAAGA(8/7)
MflI 110 AGATCC [GA]^GATC[CT]
MseI 353 TTAA T^TAA
MvnI 172 CGCG CG^CG
NdeII 111 GATC ^GATC
PflMI 114 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 28 CAGCTG CAG^CTG
SapI 96 GAAGAGC GCTCTTC(1/4)
Sau3AI 111 GATC ^GATC
SfcI 522 CTACAG C^T[GA][CT]AG
SnaBI 393 TACGTA TAC^GTA
StyI 497 CCAAGG C^C[AT][AT]GG
TfiI 582 GAATC G^A[AT]TC
Tru9I 353 TTAA T^TAA
Tsp45I 214 GTGAC ^GT[GC]AC
Van91I 114 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 497 CCAAGGGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 110 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
ApaI 249 GGGCCC GGGCC^C
448 GGGCCC
BpmI 256 CTGGAG CTGGAG(16/14)
280 CTGGAG
BsmFI 199 GTCCC GGGAC(10/14)
534 GTCCC
BsrI 217 ACTGG ACTGG(1/-1)
602 ACTGG
HincII 73 GTCAAC GT[CT]^[GA]AC
352 GTTAAC
HindII 73 GTCAAC GT[CT]^[GA]AC
352 GTTAAC
HphI 213 GGTGA GGTGA(8/7)
587 TCACC
MspA1I 28 CAGCTG C[AC]G^C[GT]G
44 CAGCGG
NlaIII 375 CATG CATG^
432 CATG
SfaNI 101 GCATC GCATC(5/9)
527 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeII MnlI
MspI MvaI MwoI NciI NlaIV
RsaI Sau96I ScrFI SmaI TseI
XmaCI XmaI