################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 466 GTAGAC GT^[AC][GT]AC AcsI 214 AAATTC [GA]^AATT[CT] ApoI 214 AAATTC [GA]^AATT[CT] BanI 97 GGCACC G^G[CT][GA]CC BbvI 436 GCAGC GCAGC(8/12) BsaI 22 GAGACC GGTCTC(1/5) BseRI 19 GAGGAG GAGGAG(10/8) BsiEI 123 CGACCG CG[GA][CT]^CG BsiYI 384 CCAGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 384 CCAGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 529 GAGACG CGTCTC(1/5) BspMI 202 ACCTGC ACCTGC(4/8) BsrFI 86 ACCGGC [GA]^CCGG[CT] BstAPI 268 GCACCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 414 CATCC GGATG(2/0) Cac8I 159 GCTGGC GC[ACGT]^[ACGT]GC Cfr10I 86 ACCGGC [GA]^CCGG[CT] DdeI 377 CTCAG C^T[ACGT]AG DraII 199 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 161 TGGCCG [CT]^GGCC[GA] EcoO109I 199 GGGACCT [GA]G^G[ACGT]CC[CT] FokI 414 CATCC GGATG(9/13) HincII 534 GTTGAC GT[CT]^[GA]AC HindII 534 GTTGAC GT[CT]^[GA]AC MaeIII 247 GTCAC ^GT[ACGT]AC MnlI 19 GAGG CCTC(7/6) PflMI 384 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 199 GGGACCT [GA]G^G[AT]CC[CT] PshAI 24 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 456 CTGCAG CTGCA^G SfcI 456 CTGCAG C^T[GA][CT]AG SspI 169 AATATT AAT^ATT TaqI 357 TCGA T^CGA TseI 436 GCAGC G^C[AT]GC Tsp45I 247 GTCAC ^GT[GC]AC Tsp509I 215 AATT ^AATT TspRI 363 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 357 TCGA T^CGA Van91I 384 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 473 CCATGGCGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AvaII 84 GGACC G^G[AT]CC 200 GGACC BcnI 126 CCGGG CC^[GC]GG 176 CCGGG BsgI 115 CTGCAC GTGCAG(16/14) 305 GTGCAG BsmAI 22 GAGAC GTCTC(1/5) 529 GAGAC BsmFI 179 GGGAC GGGAC(10/14) 199 GGGAC BstNI 301 CCTGG CC^[AT]GG 384 CCAGG DsaI 404 CCATGG C^C[GA][CT]GG 473 CCATGG EarI 16 GAAGAG CTCTTC(1/4) 526 GAAGAG EcoRII 301 CCTGG ^CC[AT]GG 384 CCAGG EcoT14I 404 CCATGG C^C[AT][AT]GG 473 CCATGG HinfI 66 GAATC G^A[ACGT]TC 359 GAATC Ksp632I 16 GAAGAG CTCTTC(1/4) 526 GAAGAG MaeII 245 ACGT A^CGT 532 ACGT MvaI 301 CCTGG CC^[AT]GG 384 CCAGG MwoI 186 GCTGGTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 268 GCACCTTATGC NciI 126 CCGGG CC^[GC]GG 176 CCGGG NcoI 404 CCATGG C^CATGG 473 CCATGG StyI 404 CCATGG C^C[AT][AT]GG 473 CCATGG TfiI 66 GAATC G^A[AT]TC 359 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AfaI AluI BsaJI BssKI Cfr13I Fnu4HI HaeIII HapII HpaII ItaI MboII MspI NlaIII NlaIV RsaI Sau96I ScrFI