Restriction map for
pFN21AE5210
(Circular) (Six-base) MAPSORT of: pFN21AE5210.seq Check: 3636 from: 1 to: 5245
pFN21AE5210 5245 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2472 2588 1937
Size: 535 116 4594
Fragments arranged by size:
4594 535 116
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 3372 3745 1563
Size: 1809 373 3063
Fragments arranged by size:
3063 1809 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 3308 4692 4960 1632
Size: 408 1268 1384 268 1917
Fragments arranged by size:
1917 1384 1268 408 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5048
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5245
AlwNI CAG_nnn'CTG
Cuts at: 2988 4560 2988
Size: 1572 3673
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2219 2554 2780 2932 5041 1493
Size: 726 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 726 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 1870
Size: 73 627 4545
Fragments arranged by size:
4545 627 73
BamHI G'GATC_C
Cuts at: 2575 2942 2575
Size: 367 4878
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2566 4021 618
Size: 325 217 627 315 464 1455 1842
Fragments arranged by size:
1842 1455 627 464 325 315 217
BanII G_rGCy'C
Cuts at: 1391 2564 1391
Size: 1173 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3436 3470 1903
Size: 34 50 34 1415 34 3678
Fragments arranged by size:
3678 1415 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 4347 3137
Size: 1210 4035
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3856 136
Size: 107 122 71 631 605 2184 1525
Fragments arranged by size:
2184 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5240 5240
Size: 5245
BlpI GC'TnA_GC
Cuts at: 2662 2662
Size: 5245
Bme1580I G_kGCm'C
Cuts at: 1790 3306 4462 1790
Size: 1516 1156 2573
Fragments arranged by size:
2573 1516 1156
BmgBI CAC'GTC
Cuts at: 1318 2251 1318
Size: 933 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3934 442
Size: 416 53 83 399 24 1559 958 1753
Fragments arranged by size:
1753 1559 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3905 1239
Size: 2666 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 3908 882
Size: 617 522 1887 2219
Fragments arranged by size:
2219 1887 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 3036 493
Size: 711 1832 2702
Fragments arranged by size:
2702 1832 711
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3052 3434 4069 275
Size: 53 83 186 936 1519 382 635 1451
Fragments arranged by size:
1519 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3678 4076 4350 4497 4908 3678
Size: 398 274 147 411 4015
Fragments arranged by size:
4015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5020
Fragments arranged by size:
5020 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 1186
Size: 853 4392
BseYI C'CCAG_C
Cuts at: 2200 4448 4944 2200
Size: 2248 496 2501
Fragments arranged by size:
2501 2248 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2331 1163
Size: 573 369 226 4077
Fragments arranged by size:
4077 573 369 226
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 2695 3456 4484 1540
Size: 275 186 130 564 761 1028 2301
Fragments arranged by size:
2301 1028 761 564 275 186 130
BsiHKAI G_wGCw'C
Cuts at: 3306 3391 4462 3306
Size: 85 1071 4089
Fragments arranged by size:
4089 1071 85
BsmI GAATG_Cn'
Cuts at: 2756 2849 2756
Size: 93 5152
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2528
Size: 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 3306 3391 4462 1391
Size: 399 774 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 399 85
BspEI T'CCGG_A
Cuts at: 4908 4908
Size: 5245
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 2600 844
Size: 1372 384 3489
Fragments arranged by size:
3489 1372 384
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 1394
Size: 429 1309 3507
Fragments arranged by size:
3507 1309 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 3914 65
Size: 1183 2492 174 1396
Fragments arranged by size:
2492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 3889 4076 1700
Size: 110 281 1798 187 2869
Fragments arranged by size:
2869 1798 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 3305 4317 3305
Size: 1012 4233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 794
Size: 1485 3760
BstBI TT'CG_AA
Cuts at: 4049 4049
Size: 5245
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2575 2942 3327 3344 4796 4882 4894 5240
Size: 603 367 385 17 1452 86 12 346
Cuts at: 5240 1972
Size: 1977
Fragments arranged by size:
1977 1452 603 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2409 2478 513
Size: 552 1344 69 3280
Fragments arranged by size:
3280 1344 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4506
Fragments arranged by size:
4506 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3555 3575 2761
Size: 794 20 4431
Fragments arranged by size:
4431 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2549 2549
Size: 5245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2692 3581
Size: 54 1425 50 275 18 336 526 889
Cuts at: 3581 5210 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 526 336 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2526 3175 1353
Size: 663 510 649 3423
Fragments arranged by size:
3423 663 649 510
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3832 4206 4352 387
Size: 1064 138 2243 374 146 1280
Fragments arranged by size:
2243 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 3308 3905 4692 4960 1239
Size: 393 408 1268 597 787 268 1524
Fragments arranged by size:
1524 1268 787 597 408 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2205 2205
Size: 5245
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 4392 1678
Size: 2714 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3886 3918 3960 3992 1373
Size: 32 135 32 2314 32 42 32 2626
Fragments arranged by size:
2626 2314 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 2589 2835 669
Size: 988 281 603 48 246 3079
Fragments arranged by size:
3079 988 603 281 246 48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3423
Fragments arranged by size:
3423 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3562 3721 518
Size: 648 2037 359 159 2042
Fragments arranged by size:
2042 2037 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2661 3338 4486 4731 1606
Size: 1055 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2409 2478 513
Size: 552 1344 69 3280
Fragments arranged by size:
3280 1344 552 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2603 4148 1132
Size: 97 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5245
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 3958
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2205
Size: 5245
PshAI GACnn'nnGTC
Cuts at: 1613 2033 1613
Size: 420 4825
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2465 2597 830
Size: 1635 132 3478
Fragments arranged by size:
3478 1635 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SbfI CC_TGCA'GG
Cuts at: 2597 2597
Size: 5245
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SfcI C'TryA_G
Cuts at: 826 1045 2461 2593 3728 4409 4600 826
Size: 219 1416 132 1135 681 191 1471
Fragments arranged by size:
1471 1416 1135 681 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2572 1872
Size: 700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4250 4512 820
Size: 197 926 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2603 1132
Size: 1471 3774
SspI AAT'ATT
Cuts at: 5 52 2176 3169 5
Size: 47 2124 993 2081
Fragments arranged by size:
2124 2081 993 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5245
StyI C'CwwG_G
Cuts at: 513 1065 2409 2478 2684 513
Size: 552 1344 69 206 3074
Fragments arranged by size:
3074 1344 552 206 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 3289 3442 3627 4072 325
Size: 1255 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2403 3491
Size: 274 80 33 51 1443 159 267 1088
Cuts at: 3491 5203 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1088 274 267 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5205 5237 5205
Size: 32 5213
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5245
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 5245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2570 1870
Size: 700 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI