Restriction map for
pFN21AB6937
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MamI MfeI MflI MluI
MroI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 301 GTCTAC GT^[AC][GT]AC
AccII 48 CGCG CG^CG
AcsI 537 GAATTT [GA]^AATT[CT]
AflIII 407 ACATGT A^C[GA][CT]GT
AluI 359 AGCT AG^CT
AlwI 525 GATCC GGATC(4/5)
Aor51HI 431 AGCGCT AGC^GCT
ApaI 66 GGGCCC GGGCC^C
ApoI 537 GAATTT [GA]^AATT[CT]
BbsI 241 GTCTTC GAAGAC(2/6)
BfaI 154 CTAG C^TAG
BpuAI 241 GTCTTC GAAGAC(2/6)
BsaAI 331 TACGTG [CT]AC^GT[GA]
BsmAI 396 GTCTC GTCTC(1/5)
BspLU11I 407 ACATGT A^CATGT
BspMI 382 ACCTGC ACCTGC(4/8)
BsrFI 170 GCCGGC [GA]^CCGG[CT]
BstUI 48 CGCG CG^CG
Cfr10I 170 GCCGGC [GA]^CCGG[CT]
DdeI 263 CTGAG C^T[ACGT]AG
DpnII 525 GATC ^GATC
DraII 65 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 47 CCGCGG C^C[GA][CT]GG
EarI 322 CTCTTC CTCTTC(1/4)
Eco47III 431 AGCGCT AGC^GCT
EcoO109I 65 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 599 CCAAGG C^C[AT][AT]GG
Ksp632I 322 CTCTTC CTCTTC(1/4)
KspI 47 CCGCGG CCGC^GG
MaeI 154 CTAG C^TAG
MaeIII 509 GTGAC ^GT[ACGT]AC
MboI 525 GATC ^GATC
MvnI 48 CGCG CG^CG
NaeI 170 GCCGGC GCC^GGC
NdeII 525 GATC ^GATC
NgoMIV 170 GCCGGC G^CCGGC
NspI 407 ACATGT [GA]CATG^[CT]
PaeR7I 83 CTCGAG C^TCGAG
PleI 394 GAGTC GAGTC(4/5)
PshAI 294 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 47 CCGCGG CCGC^GG
Sau3AI 525 GATC ^GATC
SfaNI 363 GATGC GCATC(5/9)
SfiI 45 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 33 CCCGGG CCC^GGG
SmlI 83 CTCGAG C^T[CT][GA]AG
StuI 14 AGGCCT AGG^CCT
StyI 599 CCAAGG C^C[AT][AT]GG
TfiI 498 GATTC G^A[AT]TC
Tsp45I 509 GTGAC ^GT[GC]AC
XhoI 83 CTCGAG C^TCGAG
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 72 GGCGCC G[GA]^CG[CT]C
167 GGCGCC
BalI 19 TGGCCA TGG^CCA
179 TGGCCA
BanI 72 GGCGCC G^G[CT][GA]CC
167 GGCGCC
BbeI 72 GGCGCC GGCGC^C
167 GGCGCC
BglI 46 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
96 GCCCTCGGGGC
BpmI 442 CTGGAG CTGGAG(16/14)
502 CTGGAG
BsaHI 72 GGCGCC G[GA]^CG[CT]C
167 GGCGCC
EaeI 19 TGGCCA [CT]^GGCC[GA]
179 TGGCCA
Hin1I 72 GGCGCC G[GA]^CG[CT]C
167 GGCGCC
HinfI 394 GAGTC G^A[ACGT]TC
498 GATTC
HphI 534 GGTGA GGTGA(8/7)
380 TCACC
KasI 72 GGCGCC G^GCGCC
167 GGCGCC
MluNI 19 TGGCCA TGG^CCA
179 TGGCCA
MscI 19 TGGCCA TGG^CCA
179 TGGCCA
MseI 412 TTAA T^TAA
611 TTAA
MspA1I 4 CAGCGG C[AC]G^C[GT]G
47 CCGCGG
NarI 72 GGCGCC GG^CGCC
167 GGCGCC
SfoI 72 GGCGCC GGC^GCC
167 GGCGCC
TaqI 84 TCGA T^CGA
230 TCGA
Tru9I 412 TTAA T^TAA
611 TTAA
Tsp509I 538 AATT ^AATT
609 AATT
TthHB8I 84 TCGA T^CGA
230 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AvaI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI