Restriction map for
pFN21AB6937
(Linear) (Six-base) MAPSORT of: KIBB6937.seq Check: 558 from: 1 to: 630
KIBB6937.orf
With 182 enzymes: SgfI *
December 24, 2009 08:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 391 630
Size: 391 239
AccI GT'mk_AC
Cuts at: 0 302 630
Size: 302 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 630
Size: 574 56
AfeI AGC'GCT
Cuts at: 0 433 630
Size: 433 197
AflIII A'CryG_T
Cuts at: 0 407 630
Size: 407 223
ApaI G_GGCC'C
Cuts at: 0 70 630
Size: 70 560
ApoI r'AATT_y
Cuts at: 0 537 630
Size: 537 93
AvaI C'yCGr_G
Cuts at: 0 10 33 83 99 630
Size: 10 23 50 16 531
Fragments arranged by size:
531 50 23 16 10
BanI G'GyrC_C
Cuts at: 0 72 167 630
Size: 72 95 463
Fragments arranged by size:
463 95 72
BanII G_rGCy'C
Cuts at: 0 70 98 343 630
Size: 70 28 245 287
Fragments arranged by size:
287 245 70 28
BbeI G_GCGC'C
Cuts at: 0 76 171 630
Size: 76 95 459
Fragments arranged by size:
459 95 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 630
Size: 234 396
BglI GCCn_nnn'nGGC
Cuts at: 0 52 102 630
Size: 52 50 528
Fragments arranged by size:
528 52 50
Bme1580I G_kGCm'C
Cuts at: 0 70 630
Size: 70 560
BmgBI CAC'GTC
Cuts at: 0 25 630
Size: 25 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 523 630
Size: 463 60 107
Fragments arranged by size:
463 107 60
BsaAI yAC'GTr
Cuts at: 0 333 630
Size: 333 297
BsaHI Gr'CG_yC
Cuts at: 0 73 168 630
Size: 73 95 462
Fragments arranged by size:
462 95 73
BseYI C'CCAG_C
Cuts at: 0 515 630
Size: 515 115
Bsp1286I G_dGCh'C
Cuts at: 0 70 98 343 630
Size: 70 28 245 287
Fragments arranged by size:
287 245 70 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 391 630
Size: 391 239
BsrFI r'CCGG_y
Cuts at: 0 170 630
Size: 170 460
BtgI C'CryG_G
Cuts at: 0 47 630
Size: 47 583
EaeI y'GGCC_r
Cuts at: 0 19 179 630
Size: 19 160 451
Fragments arranged by size:
451 160 19
EarI CTCTTCn'nnn_
Cuts at: 0 328 630
Size: 328 302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 523 574 630
Size: 463 60 51 56
Fragments arranged by size:
463 60 56 51
EcoO109I rG'GnC_Cy
Cuts at: 0 66 630
Size: 66 564
HaeII r_GCGC'y
Cuts at: 0 76 171 435 630
Size: 76 95 264 195
Fragments arranged by size:
264 195 95 76
KasI G'GCGC_C
Cuts at: 0 72 167 630
Size: 72 95 463
Fragments arranged by size:
463 95 72
MscI TGG'CCA
Cuts at: 0 21 181 630
Size: 21 160 449
Fragments arranged by size:
449 160 21
MspA1I CmG'CkG
Cuts at: 0 6 49 630
Size: 6 43 581
Fragments arranged by size:
581 43 6
NaeI GCC'GGC
Cuts at: 0 172 630
Size: 172 458
NarI GG'CG_CC
Cuts at: 0 73 168 630
Size: 73 95 462
Fragments arranged by size:
462 95 73
NgoMIV G'CCGG_C
Cuts at: 0 170 630
Size: 170 460
NspI r_CATG'y
Cuts at: 0 411 630
Size: 411 219
PciI A'CATG_T
Cuts at: 0 407 630
Size: 407 223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 630
Size: 110 32 488
Fragments arranged by size:
488 110 32
PshAI GACnn'nnGTC
Cuts at: 0 298 630
Size: 298 332
PspOMI G'GGCC_C
Cuts at: 0 66 630
Size: 66 564
PspXI vC'TCGA_Gb
Cuts at: 0 83 630
Size: 83 547
SacII CC_GC'GG
Cuts at: 0 50 630
Size: 50 580
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 52 630
Size: 52 578
SfoI GGC'GCC
Cuts at: 0 74 169 630
Size: 74 95 461
Fragments arranged by size:
461 95 74
SmaI CCC'GGG
Cuts at: 0 35 630
Size: 35 595
SmlI C'TyrA_G
Cuts at: 0 83 630
Size: 83 547
StuI AGG'CCT
Cuts at: 0 16 630
Size: 16 614
StyI C'CwwG_G
Cuts at: 0 599 630
Size: 599 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 252 284 630
Size: 252 32 346
Fragments arranged by size:
346 252 32
XhoI C'TCGA_G
Cuts at: 0 83 630
Size: 83 547
XmaI C'CCGG_G
Cuts at: 0 33 630
Size: 33 597
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII ApaI ApoI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BmgBI BpmI
BsaAI BsaHI BseYI Bsp1286I BspMI BsrFI BtgI EaeI
EarI Eco57MI EcoO109I HaeII KasI MscI MspA1I NaeI
NarI NgoMIV NspI PciI PpiI PshAI PspOMI PspXI
SacII SfiI SfoI SmaI SmlI StuI StyI TstI
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI
XmnI ZraI