Restriction map for
pFN21AB4810
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MunI MvnI
MwoI NarI NciI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 511 GTCTAC GT^[AC][GT]AC
AfaI 252 GTAC GT^AC
AlwNI 195 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 681 GAGCAC G[AT]GC[AT]^C
BalI 56 TGGCCA TGG^CCA
BanII 397 GAGCCC G[GA]GC[CT]^C
BsaBI 171 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 460 CTGCAC GTGCAG(16/14)
BsiHKAI 681 GAGCAC G[AT]GC[AT]^C
BsmBI 374 GAGACG CGTCTC(1/5)
BsmI 117 GAATGC GAATGC(1/-1)
BspDI 493 ATCGAT AT^CGAT
BsrFI 574 GCCGGC [GA]^CCGG[CT]
BsrI 826 CCAGT ACTGG(1/-1)
BssSI 166 CTCGTG CACGAG(-5/-1)
BstXI 206 CCATACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 50 GCGC GCG^C
Cfr10I 574 GCCGGC [GA]^CCGG[CT]
ClaI 493 ATCGAT AT^CGAT
DraI 835 TTTAAA TTT^AAA
EaeI 56 TGGCCA [CT]^GGCC[GA]
EcoT14I 122 CCATGG C^C[AT][AT]GG
HaeII 49 AGCGCC [GA]GCGC^[CT]
HapII 575 CCGG C^CGG
HgaI 376 GACGC GACGC(5/10)
HhaI 50 GCGC GCG^C
HinP1I 50 GCGC G^CGC
HindIII 776 AAGCTT A^AGCTT
HpaII 575 CCGG C^CGG
MamI 171 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MspA1I 531 CCGCTG C[AC]G^C[GT]G
MspI 575 CCGG C^CGG
NaeI 574 GCCGGC GCC^GGC
NcoI 122 CCATGG C^CATGG
NdeI 695 CATATG CA^TATG
NgoMIV 574 GCCGGC G^CCGGC
PpuMI 85 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 579 CTGCAG CTGCA^G
RsaI 252 GTAC GT^AC
SapI 678 GAAGAGC GCTCTTC(1/4)
SmlI 618 CTTGAG C^T[CT][GA]AG
StuI 599 AGGCCT AGG^CCT
StyI 122 CCATGG C^C[AT][AT]GG
TaqI 494 TCGA T^CGA
TthHB8I 494 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 65 GGATC GGATC(4/5)
197 GGATC
AvaII 86 GGTCC G^G[AT]CC
654 GGACC
BbsI 109 GAAGAC GAAGAC(2/6)
846 GTCTTC
BmyI 397 GAGCCC G[AGT]GC[ACT]^C
681 GAGCAC
BpuAI 109 GAAGAC GAAGAC(2/6)
846 GTCTTC
BsiYI 531 CCGCTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
591 CCCCAAGAAGG
BslI 531 CCGCTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
591 CCCCAAGAAGG
BsmAI 374 GAGAC GTCTC(1/5)
475 GAGAC
BsmFI 653 GGGAC GGGAC(10/14)
589 GTCCC
Bsp1286I 397 GAGCCC G[AGT]GC[ACT]^C
681 GAGCAC
BstYI 65 GGATCT [GA]^GATC[CT]
197 GGATCT
DdeI 712 CTTAG C^T[ACGT]AG
886 CTAAG
DsaI 122 CCATGG C^C[GA][CT]GG
668 CCACGG
MflI 65 GGATCT [GA]^GATC[CT]
197 GGATCT
MseI 485 TTAA T^TAA
836 TTAA
SfcI 579 CTGCAG C^T[GA][CT]AG
841 CTACAG
Tru9I 485 TTAA T^TAA
836 TTAA
XhoII 65 GGATCT [GA]^GATC[CT]
197 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BseRI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI Tsp45I Tsp509I TspRI