(Circular) (Six-base) MAPSORT of: pFN21AB4810.seq Check: 7896 from: 1 to: 5620 pFN21AB4810 5620 bp With 182 enzymes: SgfI * January 17, 2012 16:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2517 2963 1937 Size: 580 446 4594 Fragments arranged by size: 4594 580 446 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3683 5067 5335 1632 Size: 1185 866 1384 268 1917 Fragments arranged by size: 1917 1384 1185 866 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2525 2675 1166 Size: 1359 150 4111 Fragments arranged by size: 4111 1359 150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 2205 3363 4935 2205 Size: 1158 1572 2890 Fragments arranged by size: 2890 1572 1158 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2236 2551 2837 2929 3155 3307 5416 1493 Size: 743 315 286 92 226 152 2109 1697 Fragments arranged by size: 2109 1697 743 315 286 226 152 92 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2406 2939 1391 Size: 1015 533 4072 Fragments arranged by size: 4072 1015 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2844 928 Size: 971 222 723 3704 Fragments arranged by size: 3704 971 723 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 2527 1318 Size: 1209 4411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4280 1239 Size: 3041 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2644 3745 4610 4908 2644 Size: 1101 865 298 3356 Fragments arranged by size: 3356 1101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 2180 5614 2180 Size: 3434 2186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2030 2362 2816 1186 Size: 841 3 332 454 3990 Fragments arranged by size: 3990 841 454 332 3 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 1163 Size: 573 714 4333 Fragments arranged by size: 4333 714 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2690 3681 3766 4837 2690 Size: 991 85 1071 3473 Fragments arranged by size: 3473 1071 991 85 BsmI GAATG_Cn' Cuts at: 2128 3131 3224 2128 Size: 1003 93 4524 Fragments arranged by size: 4524 1003 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2373 2373 Size: 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2406 2690 2939 3681 3766 4837 1391 Size: 399 616 284 249 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 616 399 284 249 85 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2579 4264 4451 1700 Size: 110 769 1685 187 2869 Fragments arranged by size: 2869 1685 769 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 2171 3680 4692 2171 Size: 1509 1012 3099 Fragments arranged by size: 3099 1509 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2218 2218 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2070 2202 2950 3317 3702 3719 5171 5257 Size: 98 132 748 367 385 17 1452 86 Cuts at: 5257 5269 5615 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 748 385 367 346 132 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2127 2673 513 Size: 552 1062 546 3460 Fragments arranged by size: 3460 1062 552 546 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2433 3136 3930 3950 2024 Size: 409 703 794 20 3694 Fragments arranged by size: 3694 794 703 409 20 ClaI AT'CG_AT Cuts at: 1554 2499 1554 Size: 945 4675 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2842 2924 2842 Size: 82 5538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 3067 3956 Size: 54 1425 50 275 18 231 1006 889 Cuts at: 3956 5585 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1006 889 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2678 2793 3550 1353 Size: 986 339 115 757 3423 Fragments arranged by size: 3423 986 757 339 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2561 4207 4581 4727 387 Size: 1064 138 972 1646 374 146 1280 Fragments arranged by size: 1646 1280 1064 972 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2817 3683 4280 5067 5335 1239 Size: 393 1185 866 597 787 268 1524 Fragments arranged by size: 1524 1185 866 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2091 2806 2859 2091 Size: 715 53 4852 Fragments arranged by size: 4852 715 53 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 2058 4767 1678 Size: 380 2709 2531 Fragments arranged by size: 2709 2531 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2260 2292 2338 2370 2715 Size: 32 135 32 688 32 46 32 345 Cuts at: 2715 2747 4261 4293 4335 4367 1373 Size: 32 1514 32 42 32 2626 Fragments arranged by size: 2626 1514 688 345 135 46 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 2781 748 Size: 2033 3587 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 10 Size: 54 1425 343 231 3567 Fragments arranged by size: 3567 1425 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2216 2361 2525 2561 2675 3578 Size: 648 1005 45 145 164 36 114 903 Cuts at: 3578 3937 4096 518 Size: 359 159 2042 Fragments arranged by size: 2042 1005 903 648 359 164 159 145 114 45 36 MspA1I CmG'CkG Cuts at: 1606 2538 3036 3713 4861 5106 1606 Size: 932 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 677 498 245 NaeI GCC'GGC Cuts at: 2581 2581 Size: 5620 NcoI C'CATG_G Cuts at: 513 1065 2127 513 Size: 552 1062 4006 Fragments arranged by size: 4006 1062 552 NdeI CA'TA_TG Cuts at: 387 2701 387 Size: 2314 3306 NgoMIV G'CCGG_C Cuts at: 2579 2579 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2091 Size: 5620 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2588 2972 830 Size: 1758 384 3478 Fragments arranged by size: 3478 1758 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SapI GCTCTTCn'nnn_ Cuts at: 2678 2678 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2584 2846 2968 4103 4784 4975 826 Size: 219 1539 262 122 1135 681 191 1471 Fragments arranged by size: 1539 1471 1135 681 262 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2623 3724 4625 4887 820 Size: 197 926 680 1101 901 262 1553 Fragments arranged by size: 1553 1101 926 901 680 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2606 1517 Size: 1089 4531 StyI C'CwwG_G Cuts at: 513 1065 2127 3059 513 Size: 552 1062 932 3074 Fragments arranged by size: 3074 1062 932 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2555 3647 3664 3817 4002 4447 325 Size: 1255 975 1092 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1092 975 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI