################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI AlwI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MroI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 571 GACGTC GACGT^C AccII 138 CGCG CG^CG AciI 10 CCGC CCGC(-3/-1) AcyI 571 GACGTC G[GA]^CG[CT]C AfaI 27 GTAC GT^AC AgeI 354 ACCGGT A^CCGGT Alw44I 88 GTGCAC G^TGCAC Aor51HI 80 AGCGCT AGC^GCT ApaLI 88 GTGCAC G^TGCAC BalI 374 TGGCCA TGG^CCA BpmI 507 CTCCAG CTGGAG(16/14) BsaAI 85 TACGTG [CT]AC^GT[GA] BsaHI 571 GACGTC G[GA]^CG[CT]C BsaWI 354 ACCGGT [AT]^CCGG[AT] BseRI 102 CTCCTC GAGGAG(10/8) BsmFI 158 GGGAC GGGAC(10/14) Bsp1407I 26 TGTACA T^GTACA BspHI 407 TCATGA T^CATGA BspMI 199 GCAGGT ACCTGC(4/8) BsrFI 354 ACCGGT [GA]^CCGG[CT] BsrGI 26 TGTACA T^GTACA BstUI 138 CGCG CG^CG BstXI 509 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 354 ACCGGT [GA]^CCGG[CT] EaeI 374 TGGCCA [CT]^GGCC[GA] Eco47III 80 AGCGCT AGC^GCT EcoRV 193 GATATC GAT^ATC EcoT14I 599 CCAAGG C^C[AT][AT]GG FauI 9 CCCGC CCCGC(4/6) HaeII 80 AGCGCT [GA]GCGC^[CT] Hin1I 571 GACGTC G[GA]^CG[CT]C MluNI 374 TGGCCA TGG^CCA MscI 374 TGGCCA TGG^CCA MseI 334 TTAA T^TAA MspA1I 609 CAGCTG C[AC]G^C[GT]G MvnI 138 CGCG CG^CG PinAI 354 ACCGGT A^CCGGT PpuMI 158 GGGACCC [GA]G^G[AT]CC[CT] PshAI 225 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 181 CTGCAG CTGCA^G PvuII 609 CAGCTG CAG^CTG RcaI 407 TCATGA T^CATGA RsaI 27 GTAC GT^AC SfaNI 512 GATGC GCATC(5/9) SfcI 181 CTGCAG C^T[GA][CT]AG SspBI 26 TGTACA T^GTACA StyI 599 CCAAGG C^C[AT][AT]GG TaqI 269 TCGA T^CGA Tru9I 334 TTAA T^TAA TthHB8I 269 TCGA T^CGA XcmI 119 CCACAATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 159 GGACC G^G[AT]CC 429 GGACC BbsI 63 GAAGAC GAAGAC(2/6) 265 GTCTTC BbvI 183 GCAGC GCAGC(8/12) 611 GCTGC BcnI 114 CCGGG CC^[GC]GG 162 CCCGG BpuAI 63 GAAGAC GAAGAC(2/6) 265 GTCTTC BsmAI 166 GAGAC GTCTC(1/5) 338 GTCTC BstF5I 399 GGATG GGATG(2/0) 460 GGATG DraII 5 AGGCCCC [GA]G^G[ACGT]CC[CT] 158 GGGACCC EcoO109I 5 AGGCCCC [GA]G^G[ACGT]CC[CT] 158 GGGACCC Fnu4HI 183 GCAGC GC^[ACGT]GC 611 GCTGC FokI 399 GGATG GGATG(9/13) 460 GGATG HinfI 325 GACTC G^A[ACGT]TC 556 GAGTC ItaI 183 GCAGC GC^[ACGT]GC 611 GCTGC MaeII 86 ACGT A^CGT 572 ACGT MboII 63 GAAGA GAAGA(8/7) 266 TCTTC NciI 114 CCGGG CC^[GC]GG 162 CCCGG NlaIII 222 CATG CATG^ 408 CATG PleI 325 GACTC GAGTC(4/5) 556 GAGTC SmlI 142 CTCAAG C^T[CT][GA]AG 421 CTCAAG TseI 183 GCAGC G^C[AT]GC 611 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI AspHI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I EcoRII HaeIII HapII HhaI HinP1I HpaII HphI MaeIII MnlI MspI MvaI MwoI NlaIV Sau96I ScrFI Tsp45I TspRI