(Circular) (Six-base) MAPSORT of: pFN21AE3603.seq Check: 7855 from: 1 to: 5338 pFN21AE3603 5338 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2580 3148 278 Size: 53 83 186 936 1044 568 2468 Fragments arranged by size: 2468 1044 936 568 186 83 53 AccI GT'mk_AC Cuts at: 1937 2681 1937 Size: 744 4594 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3401 4785 5053 1632 Size: 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 268 AfeI AGC'GCT Cuts at: 2087 2087 Size: 5338 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 2359 4169 2359 Size: 1810 3528 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 3081 4653 3081 Size: 1572 3766 ApaLI G'TGCA_C Cuts at: 2093 3395 4551 2093 Size: 1302 1156 2880 Fragments arranged by size: 2880 1302 1156 ApoI r'AATT_y Cuts at: 1493 2647 2873 3025 5134 1493 Size: 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2663 1870 Size: 73 720 4545 Fragments arranged by size: 4545 720 73 BamHI G'GATC_C Cuts at: 2668 3035 2668 Size: 367 4971 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2659 4114 618 Size: 325 217 627 872 1455 1842 Fragments arranged by size: 1842 1455 872 627 325 217 BanII G_rGCy'C Cuts at: 1391 2657 1391 Size: 1266 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 2263 928 Size: 971 176 188 4003 Fragments arranged by size: 4003 971 188 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3529 3563 1903 Size: 34 50 34 1508 34 3678 Fragments arranged by size: 3678 1508 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3949 136 Size: 107 122 71 631 605 337 1940 1525 Fragments arranged by size: 1940 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2097 3399 4555 1790 Size: 307 1302 1156 2573 Fragments arranged by size: 2573 1302 1156 307 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2170 3069 4027 Size: 416 53 83 399 24 753 899 958 Cuts at: 4027 442 Size: 1753 Fragments arranged by size: 1753 958 899 753 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2497 3998 1239 Size: 1258 1501 2579 Fragments arranged by size: 2579 1501 1258 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 2411 3463 4328 4626 2132 Size: 279 1052 865 298 2844 Fragments arranged by size: 2844 1052 865 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2092 3129 493 Size: 711 888 1037 2702 Fragments arranged by size: 2702 1037 888 711 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2577 3145 3527 4162 Size: 53 83 186 936 1044 568 382 635 Cuts at: 4162 275 Size: 1451 Fragments arranged by size: 1451 1044 936 635 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2359 3771 4169 4443 4590 5001 2359 Size: 1412 398 274 147 411 2696 Fragments arranged by size: 2696 1412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 1178 Size: 30 165 30 612 30 4471 Fragments arranged by size: 4471 612 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 1186 Size: 912 4426 BseYI C'CCAG_C Cuts at: 4541 5037 4541 Size: 496 4842 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4765 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2788 3549 4577 1540 Size: 275 186 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2097 2157 2412 2454 3399 3484 4555 2028 Size: 69 60 255 42 945 85 1071 2811 Fragments arranged by size: 2811 1071 945 255 85 69 60 42 BsmI GAATG_Cn' Cuts at: 2849 2942 2849 Size: 93 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2097 2157 2412 2454 2657 3399 Size: 399 238 69 60 255 42 203 742 Cuts at: 3399 3484 4555 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 255 238 203 85 69 60 42 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 2412 3227 4957 2412 Size: 815 1730 2793 Fragments arranged by size: 2793 1730 815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2195 2693 844 Size: 1351 498 3489 Fragments arranged by size: 3489 1351 498 BsrBI CCG'CTC Cuts at: 1394 1823 3225 1394 Size: 429 1402 3507 Fragments arranged by size: 3507 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2359 3982 4169 1700 Size: 110 549 1623 187 2869 Fragments arranged by size: 2869 1623 549 187 110 BsrGI T'GTAC_A Cuts at: 96 2031 96 Size: 1935 3403 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5338 BssSI C'ACGA_G Cuts at: 3398 4410 3398 Size: 1012 4326 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5338 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2521 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333 Size: 696 367 385 17 1452 86 12 346 Cuts at: 5333 1972 Size: 1977 Fragments arranged by size: 1977 1452 696 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4786 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2310 2854 3648 3668 2310 Size: 544 794 20 3980 Fragments arranged by size: 3980 794 544 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2115 2150 529 Size: 35 1551 35 3717 Fragments arranged by size: 3717 1551 35 35 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2785 3674 Size: 54 1425 50 275 18 549 406 889 Cuts at: 3674 5303 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 549 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2785 1537 Size: 275 973 4090 Fragments arranged by size: 4090 973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3268 1353 Size: 1915 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3925 4299 4445 387 Size: 1064 138 2336 374 146 1280 Fragments arranged by size: 2336 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2497 3401 3998 4785 5053 1239 Size: 393 865 904 597 787 268 1524 Fragments arranged by size: 1524 904 865 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2011 2164 2011 Size: 153 5185 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 EcoRV GAT'ATC Cuts at: 2200 2200 Size: 5338 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 2089 4485 1678 Size: 411 2396 2531 Fragments arranged by size: 2531 2396 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2495 2527 3979 Size: 32 135 32 743 32 148 32 1452 Cuts at: 3979 4011 4053 4085 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1452 743 148 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 2967 Fragments arranged by size: 2967 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3296 3655 3814 518 Size: 648 2130 359 159 2042 Fragments arranged by size: 2130 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2616 2754 3431 4579 4824 1606 Size: 1010 138 677 1148 245 2120 Fragments arranged by size: 2120 1148 1010 677 245 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4786 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2696 4241 1132 Size: 97 1467 1545 2229 Fragments arranged by size: 2229 1545 1467 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5338 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2125 3407 3439 4920 4952 2093 Size: 32 1282 32 1481 32 2479 Fragments arranged by size: 2479 1481 1282 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 5338 PshAI GACnn'nnGTC Cuts at: 1613 2234 1613 Size: 621 4717 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PstI C_TGCA'G Cuts at: 830 2190 2690 830 Size: 1360 500 3478 Fragments arranged by size: 3478 1360 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2616 2616 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SanDI GG'GwC_CC Cuts at: 2164 2164 Size: 5338 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2186 2686 3821 4502 4693 826 Size: 219 1141 500 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 681 500 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2426 3442 4343 4605 820 Size: 197 926 204 279 1016 901 262 1553 Fragments arranged by size: 1553 1016 926 901 279 262 204 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2604 2777 513 Size: 552 1539 173 3074 Fragments arranged by size: 3074 1539 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 3365 3382 3535 3720 4165 325 Size: 1255 666 1119 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1119 666 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 3584 5296 Size: 274 80 33 51 1443 54 1553 1712 Cuts at: 5296 96 Size: 138 Fragments arranged by size: 1712 1553 1443 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2131 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2578 3146 276 Size: 53 83 186 936 1044 568 2468 Fragments arranged by size: 2468 1044 936 568 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI