(Circular) (Six-base) MAPSORT of: pFN21AB6974.seq Check: 254 from: 1 to: 5434 pFN21AB6974 5434 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2120 3244 278 Size: 53 83 186 936 584 1124 2468 Fragments arranged by size: 2468 1124 936 584 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 1937 Size: 840 4594 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2298 3497 4881 5149 1632 Size: 666 1199 1384 268 1917 Fragments arranged by size: 1917 1384 1199 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AlwNI CAG_nnn'CTG Cuts at: 3177 4749 3177 Size: 1572 3862 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2452 2743 2969 3121 5230 1493 Size: 959 291 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2759 1870 Size: 73 816 4545 Fragments arranged by size: 4545 816 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2424 2457 2424 Size: 33 5401 BamHI G'GATC_C Cuts at: 2764 3131 2764 Size: 367 5067 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2755 4210 618 Size: 325 217 627 968 1455 1842 Fragments arranged by size: 1842 1455 968 627 325 217 BanII G_rGCy'C Cuts at: 1391 2753 1391 Size: 1362 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4045 136 Size: 107 122 71 631 605 2373 1525 Fragments arranged by size: 2373 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2851 2851 Size: 5434 Bme1580I G_kGCm'C Cuts at: 1790 3495 4651 1790 Size: 1705 1156 2573 Fragments arranged by size: 2573 1705 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3165 4123 442 Size: 416 53 83 399 24 1748 958 1753 Fragments arranged by size: 1753 1748 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2069 2148 2421 2693 4094 1239 Size: 821 9 79 273 272 1401 2579 Fragments arranged by size: 2579 1401 821 273 272 79 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 3559 4424 4722 2144 Size: 1415 865 298 2856 Fragments arranged by size: 2856 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4097 882 Size: 617 2598 2219 Fragments arranged by size: 2598 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 2577 5428 2577 Size: 2851 2583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2117 3241 3623 4258 Size: 53 83 186 936 584 1124 382 635 Cuts at: 4258 275 Size: 1451 Fragments arranged by size: 1451 1124 936 635 584 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3867 4265 4539 4686 5097 3867 Size: 398 274 147 411 4204 Fragments arranged by size: 4204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 1178 Size: 30 165 30 648 30 4531 Fragments arranged by size: 4531 648 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4584 BseYI C'CCAG_C Cuts at: 2096 4637 5133 2096 Size: 2541 496 2397 Fragments arranged by size: 2541 2397 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4861 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2884 3645 4673 1540 Size: 275 186 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3495 3580 4651 3495 Size: 85 1071 4278 Fragments arranged by size: 4278 1071 85 BsmI GAATG_Cn' Cuts at: 2945 3038 2945 Size: 93 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2753 3495 3580 4651 1391 Size: 399 963 742 85 1071 2174 Fragments arranged by size: 2174 1071 963 742 399 85 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 844 Size: 1945 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3321 1394 Size: 429 1498 3507 Fragments arranged by size: 3507 1498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3929 4103 65 Size: 1183 2681 174 1396 Fragments arranged by size: 2681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4078 4265 1700 Size: 110 2268 187 2869 Fragments arranged by size: 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5434 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429 Size: 792 367 385 17 1452 86 12 346 Cuts at: 5429 1972 Size: 1977 Fragments arranged by size: 1977 1452 792 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2428 513 Size: 552 1363 3519 Fragments arranged by size: 3519 1363 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5399 DraI TTT'AAA Cuts at: 2256 2738 2256 Size: 482 4952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2881 3770 5399 Size: 54 1425 50 275 18 1051 889 1629 Cuts at: 5399 8 Size: 43 Fragments arranged by size: 1629 1425 1051 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2881 1537 Size: 275 1069 4090 Fragments arranged by size: 4090 1069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2362 3364 1353 Size: 1009 1002 3423 Fragments arranged by size: 3423 1009 1002 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4021 4395 4541 387 Size: 1064 138 2432 374 146 1280 Fragments arranged by size: 2432 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2069 2148 2298 2421 2693 3497 Size: 393 428 9 79 150 123 272 804 Cuts at: 3497 4094 4881 5149 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 428 393 272 268 150 123 79 9 EcoO109I rG'GnC_Cy Cuts at: 2044 2044 Size: 5434 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 EcoRV GAT'ATC Cuts at: 2596 2596 Size: 5434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2216 2303 2216 Size: 87 5347 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 4581 1678 Size: 2903 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 4075 4107 4149 Size: 32 135 32 721 32 1750 32 42 Cuts at: 4149 4181 1373 Size: 32 2626 Fragments arranged by size: 2626 1750 721 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2778 3024 669 Size: 988 281 840 246 3079 Fragments arranged by size: 3079 988 840 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5434 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 2445 3033 2445 Size: 588 4846 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 4732 1305 Size: 3243 184 2007 Fragments arranged by size: 3243 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3612 Fragments arranged by size: 3612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 3392 3751 3910 518 Size: 648 945 1281 359 159 2042 Fragments arranged by size: 2042 1281 945 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 2729 2850 3527 4675 4920 1606 Size: 715 408 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 408 245 121 NcoI C'CATG_G Cuts at: 513 1065 2428 513 Size: 552 1363 3519 Fragments arranged by size: 3519 1363 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5434 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 2656 1108 Size: 1497 51 3886 Fragments arranged by size: 3886 1497 51 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2630 2662 3503 3535 5016 5048 2630 Size: 32 841 32 1481 32 3016 Fragments arranged by size: 3016 1481 841 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PstI C_TGCA'G Cuts at: 830 2786 830 Size: 1956 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 PvuII CAG'CTG Cuts at: 2321 2729 2321 Size: 408 5026 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SfcI C'TryA_G Cuts at: 826 1045 2782 3917 4598 4789 826 Size: 219 1737 1135 681 191 1471 Fragments arranged by size: 1737 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2761 1872 Size: 889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 3538 4439 4701 820 Size: 197 926 216 1379 901 262 1553 Fragments arranged by size: 1553 1379 926 901 262 216 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 2211 3358 5 Size: 47 2159 1147 2081 Fragments arranged by size: 2159 2081 1147 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2428 2873 513 Size: 552 1363 445 3074 Fragments arranged by size: 3074 1363 552 445 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 3461 3478 3631 3816 4261 325 Size: 1255 530 1351 17 153 185 445 1498 Fragments arranged by size: 1498 1351 1255 530 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2610 2642 5394 5426 2610 Size: 32 2752 32 2618 Fragments arranged by size: 2752 2618 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2227 2770 2227 Size: 543 4891 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5434 XmaI C'CCGG_G Cuts at: 1870 2759 1870 Size: 889 4545 XmnI GAAnn'nnTTC Cuts at: 3563 3563 Size: 5434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2118 3242 276 Size: 53 83 186 936 584 1124 2468 Fragments arranged by size: 2468 1124 936 584 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI