Restriction map for
pFN21AE1564
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BclI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmFI
BsoBI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 986 TCCGGA T^CCGGA
AflII 1087 CTTAAG C^TTAAG
AflIII 749 ACATGT A^C[GA][CT]GT
BalI 1844 TGGCCA TGG^CCA
BbvCI 1507 GCTGAGG CCTCAGC(-5/-2)
BcnI 225 CCCGG CC^[GC]GG
BfrI 1087 CTTAAG C^TTAAG
BglI 683 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 757 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 757 GCTCAGC GC^T[ACGT]AGC
BsaWI 986 TCCGGA [AT]^CCGG[AT]
BseAI 986 TCCGGA T^CCGGA
BseRI 43 CTCCTC GAGGAG(10/8)
BsgI 296 CTGCAC GTGCAG(16/14)
BsmBI 60 CGTCTC CGTCTC(1/5)
Bsp1407I 1938 TGTACA T^GTACA
BspDI 1030 ATCGAT AT^CGAT
BspEI 986 TCCGGA T^CCGGA
BspLU11I 749 ACATGT A^CATGT
BsrGI 1938 TGTACA T^GTACA
CelII 757 GCTCAGC GC^T[ACGT]AGC
ClaI 1030 ATCGAT AT^CGAT
DraI 936 TTTAAA TTT^AAA
DraIII 1058 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1550 CCACGG C^C[GA][CT]GG
EaeI 1844 TGGCCA [CT]^GGCC[GA]
EarI 837 GAAGAG CTCTTC(1/4)
EcoRI 2022 GAATTC G^AATTC
EcoT22I 171 ATGCAT ATGCA^T
FauI 23 GCGGG CCCGC(4/6)
HgaI 117 GCGTC GACGC(5/10)
HindIII 413 AAGCTT A^AGCTT
HphI 1123 GGTGA GGTGA(8/7)
Ksp632I 837 GAAGAG CTCTTC(1/4)
MaeII 300 ACGT A^CGT
MluNI 1844 TGGCCA TGG^CCA
MroI 986 TCCGGA T^CCGGA
MscI 1844 TGGCCA TGG^CCA
MspA1I 641 CAGCTG C[AC]G^C[GT]G
NciI 225 CCCGG CC^[GC]GG
NdeI 992 CATATG CA^TATG
NsiI 171 ATGCAT ATGCA^T
PpuMI 5 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 641 CAGCTG CAG^CTG
SacI 1985 GAGCTC GAGCT^C
SalI 1758 GTCGAC G^TCGAC
SexAI 438 ACCAGGT A^CC[AT]GGT
SfaNI 173 GCATC GCATC(5/9)
SfcI 693 CTACAG C^T[GA][CT]AG
SmlI 1087 CTTAAG C^T[CT][GA]AG
SspBI 1938 TGTACA T^GTACA
Tsp45I 1528 GTGAC ^GT[GC]AC
XbaI 1335 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AciI 23 GCGG CCGC(-3/-1)
907 GCGG
AfaI 1017 GTAC GT^AC
1939 GTAC
AlwI 487 GATCC GGATC(4/5)
1947 GGATC
AseI 1132 ATTAAT AT^TAAT
1930 ATTAAT
AsnI 1132 ATTAAT AT^TAAT
1930 ATTAAT
AspHI 755 GTGCTC G[AT]GC[AT]^C
1985 GAGCTC
AvaII 6 GGTCC G^G[AT]CC
1231 GGACC
BanII 1443 GGGCTC G[GA]GC[CT]^C
1985 GAGCTC
BbsI 1792 GAAGAC GAAGAC(2/6)
263 GTCTTC
BciVI 1682 GGATAC GTATCC(6/5)
1498 GTATCC
BfaI 49 CTAG C^TAG
1336 CTAG
BpuAI 1792 GAAGAC GAAGAC(2/6)
263 GTCTTC
BsiHKAI 755 GTGCTC G[AT]GC[AT]^C
1985 GAGCTC
BsmAI 41 GTCTC GTCTC(1/5)
61 GTCTC
Bst1107I 1273 GTATAC GTA^TAC
1346 GTATAC
BstXI 1847 CCACATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1999 CCATCAGTTTGG
BstYI 486 AGATCC [GA]^GATC[CT]
1947 GGATCT
BstZ17I 1273 GTATAC GTA^TAC
1346 GTATAC
Cac8I 33 GCTGGC GC[ACGT]^[ACGT]GC
1569 GCTTGC
CfoI 18 GCGC GCG^C
28 GCGC
DraII 5 GGGTCCT [GA]G^G[ACGT]CC[CT]
1688 AGGCCCT
EcoO109I 5 GGGTCCT [GA]G^G[ACGT]CC[CT]
1688 AGGCCCT
EcoT14I 1574 CCTTGG C^C[AT][AT]GG
1613 CCTTGG
HaeII 17 GGCGCT [GA]GCGC^[CT]
27 GGCGCT
HhaI 18 GCGC GCG^C
28 GCGC
HinP1I 18 GCGC G^CGC
28 GCGC
MaeI 49 CTAG C^TAG
1336 CTAG
MflI 486 AGATCC [GA]^GATC[CT]
1947 GGATCT
NspI 749 ACATGT [GA]CATG^[CT]
1518 GCATGT
PleI 1621 GACTC GAGTC(4/5)
1733 GAGTC
PshBI 1132 ATTAAT AT^TAAT
1930 ATTAAT
RsaI 1017 GTAC GT^AC
1939 GTAC
SspI 1537 AATATT AAT^ATT
1927 AATATT
StyI 1574 CCTTGG C^C[AT][AT]GG
1613 CCTTGG
XhoII 486 AGATCC [GA]^GATC[CT]
1947 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccI AcsI AluI ApoI Asp700I
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI