(Circular) (Six-base) MAPSORT of: pFN21AE1564.seq Check: 2162 from: 1 to: 6733 pFN21AE1564 6733 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4543 278 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3279 3352 3764 4076 1937 Size: 1342 73 412 312 4594 Fragments arranged by size: 4594 1342 412 312 73 Acc65I G'GTAC_C Cuts at: 4054 4054 Size: 6733 AclI AA'CG_TT Cuts at: 1563 4860 5233 1563 Size: 3297 373 3063 Fragments arranged by size: 3297 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4796 6180 6448 1632 Size: 3164 1384 268 1917 Fragments arranged by size: 3164 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3092 820 Size: 197 2075 4461 Fragments arranged by size: 4461 2075 197 AflIII A'CryG_T Cuts at: 1203 2754 5632 6609 1203 Size: 1551 2878 977 1327 Fragments arranged by size: 2878 1551 1327 977 AgeI A'CCGG_T Cuts at: 5564 5564 Size: 6733 AhdI GACnn_n'nnGTC Cuts at: 5462 5462 Size: 6733 AleI CACnn'nnGTG Cuts at: 1166 2648 3896 1166 Size: 1482 1248 4003 Fragments arranged by size: 4003 1482 1248 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6733 AlwNI CAG_nnn'CTG Cuts at: 4476 6048 4476 Size: 1572 5161 ApaLI G'TGCA_C Cuts at: 4790 5946 4790 Size: 1156 5577 ApoI r'AATT_y Cuts at: 1493 2131 2468 2523 2945 2981 3364 3731 4027 Size: 638 337 55 422 36 383 367 296 Cuts at: 4027 4042 4268 4420 6529 1493 Size: 15 226 152 2109 1697 Fragments arranged by size: 2109 1697 638 422 383 367 337 296 226 152 55 36 15 AseI AT'TA_AT Cuts at: 160 1032 3138 3936 5287 160 Size: 872 2106 798 1351 1606 Fragments arranged by size: 2106 1606 1351 872 798 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 AvaI C'yCGr_G Cuts at: 1870 1943 4058 1870 Size: 73 2115 4545 Fragments arranged by size: 4545 2115 73 BamHI G'GATC_C Cuts at: 4063 4430 4063 Size: 367 6366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4054 5509 618 Size: 325 217 627 2267 1455 1842 Fragments arranged by size: 2267 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3452 3994 4052 1391 Size: 2061 542 58 4072 Fragments arranged by size: 4072 2061 542 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2261 3804 928 Size: 971 362 1543 3857 Fragments arranged by size: 3857 1543 971 362 BbvCI CC'TCA_GC Cuts at: 3513 3513 Size: 6733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4924 4958 1903 Size: 34 2987 34 3678 Fragments arranged by size: 3678 2987 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3514 3681 4625 5835 3514 Size: 167 944 1210 4412 Fragments arranged by size: 4412 1210 944 167 BclI T'GATC_A Cuts at: 1548 1548 Size: 6733 BfrBI ATG'CAT Cuts at: 2178 2178 Size: 6733 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2694 5344 136 Size: 107 122 71 631 605 1022 2650 1525 Fragments arranged by size: 2650 1525 1022 631 605 122 107 71 BglII A'GATC_T Cuts at: 6728 6728 Size: 6733 BlpI GC'TnA_GC Cuts at: 2763 4150 2763 Size: 1387 5346 Bme1580I G_kGCm'C Cuts at: 1790 3563 3606 4794 5950 1790 Size: 1773 43 1188 1156 2573 Fragments arranged by size: 2573 1773 1188 1156 43 BmgBI CAC'GTC Cuts at: 1318 2306 1318 Size: 988 5745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2295 4464 5422 Size: 416 53 83 399 24 878 2169 958 Cuts at: 5422 442 Size: 1753 Fragments arranged by size: 2169 1753 958 878 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3466 3466 Size: 6733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2805 3144 3776 5393 1239 Size: 1566 339 632 1617 2579 Fragments arranged by size: 2579 1617 1566 632 339 Bpu10I CC'TnA_GC Cuts at: 3513 3513 Size: 6733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4858 5723 6021 4858 Size: 865 298 5570 Fragments arranged by size: 5570 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5396 882 Size: 617 3897 2219 Fragments arranged by size: 3897 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4524 493 Size: 711 3320 2702 Fragments arranged by size: 3320 2702 711 BsaBI GATnn'nnATC Cuts at: 6727 6727 Size: 6733 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4540 4922 5557 275 Size: 53 83 186 936 3007 382 635 1451 Fragments arranged by size: 3007 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2991 5166 5564 5838 5985 6396 2991 Size: 2175 398 274 147 411 3328 Fragments arranged by size: 3328 2175 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3117 3147 1178 Size: 30 165 30 1714 30 4764 Fragments arranged by size: 4764 1714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 1186 Size: 853 5880 BseYI C'CCAG_C Cuts at: 5936 6432 5936 Size: 496 6237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2286 1163 Size: 573 550 5610 Fragments arranged by size: 5610 573 550 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4183 4944 5972 1540 Size: 275 186 2182 761 1028 2301 Fragments arranged by size: 2301 2182 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2764 3994 4794 4879 5950 2764 Size: 1230 800 85 1071 3547 Fragments arranged by size: 3547 1230 1071 800 85 BsmI GAATG_Cn' Cuts at: 2180 2489 2621 2690 2747 3907 4244 4337 2180 Size: 309 132 69 57 1160 337 93 4576 Fragments arranged by size: 4576 1160 337 309 132 93 69 57 BsmBI CGTCTCn'nnnn_ Cuts at: 2071 2071 Size: 6733 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2764 3452 3563 3606 3994 4052 4794 Size: 399 974 688 111 43 388 58 742 Cuts at: 4794 4879 5950 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 974 742 688 399 388 111 85 58 43 BspEI T'CCGG_A Cuts at: 2991 6396 2991 Size: 3405 3328 BspHI T'CATG_A Cuts at: 4622 6352 4622 Size: 1730 5003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4088 844 Size: 3244 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4620 1394 Size: 429 2797 3507 Fragments arranged by size: 3507 2797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2389 3424 3840 5228 5402 65 Size: 1183 1141 1035 416 1388 174 1396 Fragments arranged by size: 1396 1388 1183 1141 1035 416 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5377 5564 1700 Size: 110 3567 187 2869 Fragments arranged by size: 3567 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3943 96 Size: 3847 2886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6733 BssSI C'ACGA_G Cuts at: 4793 5805 4793 Size: 1012 5721 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6733 BstBI TT'CG_AA Cuts at: 5537 5537 Size: 6733 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6733 BstXI CCAn_nnnn'nTGG Cuts at: 3859 4011 3859 Size: 152 6581 BstYI r'GATC_y Cuts at: 1972 2491 3952 4063 4430 4815 4832 6284 6370 Size: 519 1461 111 367 385 17 1452 86 Cuts at: 6370 6382 6728 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1461 1452 519 385 367 346 111 86 17 12 BstZ17I GTA'TAC Cuts at: 3280 3353 3280 Size: 73 6660 BtgI C'CryG_G Cuts at: 513 1065 3555 513 Size: 552 2490 3691 Fragments arranged by size: 3691 2490 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5994 Fragments arranged by size: 5994 683 56 BtsI GCAGTG_nn' Cuts at: 2295 4249 5043 5063 2295 Size: 1954 794 20 3965 Fragments arranged by size: 3965 1954 794 20 ClaI AT'CG_AT Cuts at: 1554 3036 1554 Size: 1482 5251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6698 DraI TTT'AAA Cuts at: 2943 4037 2943 Size: 1094 5639 DraIII CAC_nnn'GTG Cuts at: 3068 3068 Size: 6733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5740 809 Size: 507 4424 1802 Fragments arranged by size: 4424 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3849 4180 5069 Size: 54 1425 50 275 18 2019 331 889 Cuts at: 5069 6698 8 Size: 1629 43 Fragments arranged by size: 2019 1629 1425 889 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4180 1537 Size: 275 2368 4090 Fragments arranged by size: 4090 2368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 4663 1353 Size: 1484 1826 3423 Fragments arranged by size: 3423 1826 1484 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2927 5320 5694 5840 387 Size: 1064 138 1338 2393 374 146 1280 Fragments arranged by size: 2393 1338 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3992 3992 Size: 6733 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2805 3144 3776 4796 5393 6180 6448 Size: 393 1173 339 632 1020 597 787 268 Cuts at: 6448 1239 Size: 1524 Fragments arranged by size: 1524 1173 1020 787 632 597 393 339 268 EcoO109I rG'GnC_Cy Cuts at: 2011 3694 2011 Size: 1683 5050 EcoRI G'AATT_C Cuts at: 4027 4042 4420 4027 Size: 15 378 6340 Fragments arranged by size: 6340 378 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3107 3107 Size: 6733 FspI TGC'GCA Cuts at: 5239 5239 Size: 6733 HaeII r_GCGC'y Cuts at: 1678 2026 2036 5880 1678 Size: 348 10 3844 2531 Fragments arranged by size: 3844 2531 348 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2163 2195 2603 2635 2694 Size: 32 135 32 591 32 408 32 59 Cuts at: 2694 2726 2729 2761 3319 3351 3617 3649 3704 Size: 32 3 32 558 32 266 32 55 Cuts at: 3704 3736 5374 5406 5448 5480 1373 Size: 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 591 558 408 266 135 59 55 42 32 32 32 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 2313 3742 3765 4077 4323 669 Size: 988 281 375 1429 23 312 246 3079 Fragments arranged by size: 3079 1429 988 375 312 281 246 23 HindIII A'AGCT_T Cuts at: 748 2418 748 Size: 1670 5063 HpaI GTT'AAC Cuts at: 4323 4323 Size: 6733 KpnI G_GTAC'C Cuts at: 4058 4058 Size: 6733 MfeI C'AATT_G Cuts at: 4332 4332 Size: 6733 MluI A'CGCG_T Cuts at: 6609 6609 Size: 6733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3930 5847 6031 1305 Size: 2625 1917 184 2007 Fragments arranged by size: 2625 2007 1917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3851 10 Size: 54 1425 343 2019 2892 Fragments arranged by size: 2892 2019 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 3528 3896 3926 4691 5050 5209 Size: 648 1482 880 368 30 765 359 159 Cuts at: 5209 518 Size: 2042 Fragments arranged by size: 2042 1482 880 765 648 368 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2648 4149 4826 5974 6219 1606 Size: 1042 1501 677 1148 245 2120 Fragments arranged by size: 2120 1501 1148 1042 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6181 NdeI CA'TA_TG Cuts at: 387 2998 387 Size: 2611 4122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6733 NotI GC'GGCC_GC Cuts at: 4180 4180 Size: 6733 NsiI A_TGCA'T Cuts at: 2180 2180 Size: 6733 NspI r_CATG'y Cuts at: 1132 1229 2758 3527 4091 5636 1132 Size: 97 1529 769 564 1545 2229 Fragments arranged by size: 2229 1545 1529 769 564 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6733 PciI A'CATG_T Cuts at: 2754 5632 2754 Size: 2878 3855 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6733 PmeI GTTT'AAAC Cuts at: 4037 4037 Size: 6733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4802 4834 6315 6347 4802 Size: 32 1481 32 5188 Fragments arranged by size: 5188 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2011 Size: 6733 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6733 PsiI TTA'TAA Cuts at: 3495 3912 4303 3495 Size: 417 391 5925 Fragments arranged by size: 5925 417 391 PstI C_TGCA'G Cuts at: 830 4085 830 Size: 3255 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6733 PvuII CAG'CTG Cuts at: 2648 2648 Size: 6733 SacI G_AGCT'C Cuts at: 3994 3994 Size: 6733 SalI G'TCGA_C Cuts at: 1936 3763 4075 1936 Size: 1827 312 4594 Fragments arranged by size: 4594 1827 312 SbfI CC_TGCA'GG Cuts at: 4085 4085 Size: 6733 ScaI AGT'ACT Cuts at: 4981 6693 4981 Size: 1712 5021 SexAI A'CCwGG_T Cuts at: 2443 2443 Size: 6733 SfcI C'TryA_G Cuts at: 826 1045 2698 4081 5216 5897 6088 826 Size: 219 1653 1383 1135 681 191 1471 Fragments arranged by size: 1653 1471 1383 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6733 SmaI CCC'GGG Cuts at: 1872 4060 1872 Size: 2188 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3092 4837 5738 6000 820 Size: 197 926 1149 1745 901 262 1553 Fragments arranged by size: 1745 1553 1149 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4524 493 Size: 4031 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6733 SphI G_CATG'C Cuts at: 1132 4091 1132 Size: 2959 3774 SspI AAT'ATT Cuts at: 5 52 3544 3934 4657 5 Size: 47 3492 390 723 2081 Fragments arranged by size: 3492 2081 723 390 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6733 StyI C'CwwG_G Cuts at: 513 1065 3579 3618 4172 513 Size: 552 2514 39 554 3074 Fragments arranged by size: 3074 2514 554 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2006 3851 4760 4777 4930 5115 5560 Size: 1255 426 1845 909 17 153 185 445 Cuts at: 5560 325 Size: 1498 Fragments arranged by size: 1845 1498 1255 909 445 426 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3943 4979 6691 Size: 274 80 33 51 1443 1966 1036 1712 Cuts at: 6691 96 Size: 138 Fragments arranged by size: 1966 1712 1443 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2110 2142 6693 6725 2110 Size: 32 4551 32 2118 Fragments arranged by size: 4551 2118 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6733 XbaI T'CTAG_A Cuts at: 3340 4069 3340 Size: 729 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6733 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6733 XmaI C'CCGG_G Cuts at: 1870 4058 1870 Size: 2188 4545 XmnI GAAnn'nnTTC Cuts at: 2419 2819 3402 3801 4862 2419 Size: 400 583 399 1061 4290 Fragments arranged by size: 4290 1061 583 400 399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4541 276 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI