Restriction map for
pFN21AE1564
(Linear) (Six-base) MAPSORT of: KIEE1564.seq Check: 5939 from: 1 to: 2028
KIEE1564.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AccI GT'mk_AC
Cuts at: 0 1274 1347 1759 2028
Size: 1274 73 412 269
Fragments arranged by size:
1274 412 269 73
AflII C'TTAA_G
Cuts at: 0 1087 2028
Size: 1087 941
AflIII A'CryG_T
Cuts at: 0 749 2028
Size: 749 1279
AleI CACnn'nnGTG
Cuts at: 0 643 1891 2028
Size: 643 1248 137
Fragments arranged by size:
1248 643 137
ApoI r'AATT_y
Cuts at: 0 126 463 518 940 976 1359 1726 2022
Size: 126 337 55 422 36 383 367 296
Cuts at: 2022 2028
Size: 6
Fragments arranged by size:
422 383 367 337 296 126 55 36
6
AseI AT'TA_AT
Cuts at: 0 1133 1931 2028
Size: 1133 798 97
Fragments arranged by size:
1133 798 97
BanII G_rGCy'C
Cuts at: 0 1447 1989 2028
Size: 1447 542 39
Fragments arranged by size:
1447 542 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 256 1799 2028
Size: 256 1543 229
Fragments arranged by size:
1543 256 229
BbvCI CC'TCA_GC
Cuts at: 0 1508 2028
Size: 1508 520
BciVI GTATCCnnnnn_n'
Cuts at: 0 1509 1676 2028
Size: 1509 167 352
Fragments arranged by size:
1509 352 167
BfrBI ATG'CAT
Cuts at: 0 173 2028
Size: 173 1855
BglI GCCn_nnn'nGGC
Cuts at: 0 689 2028
Size: 689 1339
BlpI GC'TnA_GC
Cuts at: 0 758 2028
Size: 758 1270
Bme1580I G_kGCm'C
Cuts at: 0 1558 1601 2028
Size: 1558 43 427
Fragments arranged by size:
1558 427 43
BmgBI CAC'GTC
Cuts at: 0 301 2028
Size: 301 1727
BmrI ACTGGGnnnn_n'
Cuts at: 0 290 2028
Size: 290 1738
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1461 2028
Size: 1461 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1139 1771 2028
Size: 800 339 632 257
Fragments arranged by size:
800 632 339 257
Bpu10I CC'TnA_GC
Cuts at: 0 1508 2028
Size: 1508 520
BsaWI w'CCGG_w
Cuts at: 0 986 2028
Size: 986 1042
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1112 1142 2028
Size: 1112 30 886
Fragments arranged by size:
1112 886 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 2028
Size: 34 1994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 2028
Size: 281 1747
BsiHKAI G_wGCw'C
Cuts at: 0 759 1989 2028
Size: 759 1230 39
Fragments arranged by size:
1230 759 39
BsmI GAATG_Cn'
Cuts at: 0 175 484 616 685 742 1902 2028
Size: 175 309 132 69 57 1160 126
Fragments arranged by size:
1160 309 175 132 126 69 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 66 2028
Size: 66 1962
Bsp1286I G_dGCh'C
Cuts at: 0 759 1447 1558 1601 1989 2028
Size: 759 688 111 43 388 39
Fragments arranged by size:
759 688 388 111 43 39
BspEI T'CCGG_A
Cuts at: 0 986 2028
Size: 986 1042
BsrDI GCAATG_nn'
Cuts at: 0 384 1419 1835 2028
Size: 384 1035 416 193
Fragments arranged by size:
1035 416 384 193
BsrGI T'GTAC_A
Cuts at: 0 1938 2028
Size: 1938 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1854 2006 2028
Size: 1854 152 22
Fragments arranged by size:
1854 152 22
BstYI r'GATC_y
Cuts at: 0 486 1947 2028
Size: 486 1461 81
Fragments arranged by size:
1461 486 81
BstZ17I GTA'TAC
Cuts at: 0 1275 1348 2028
Size: 1275 73 680
Fragments arranged by size:
1275 680 73
BtgI C'CryG_G
Cuts at: 0 1550 2028
Size: 1550 478
BtsI GCAGTG_nn'
Cuts at: 0 290 2028
Size: 290 1738
ClaI AT'CG_AT
Cuts at: 0 1031 2028
Size: 1031 997
DraI TTT'AAA
Cuts at: 0 938 2028
Size: 938 1090
DraIII CAC_nnn'GTG
Cuts at: 0 1063 2028
Size: 1063 965
EaeI y'GGCC_r
Cuts at: 0 1844 2028
Size: 1844 184
EarI CTCTTCn'nnn_
Cuts at: 0 832 2028
Size: 832 1196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 922 2028
Size: 922 1106
EcoICRI GAG'CTC
Cuts at: 0 1987 2028
Size: 1987 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1139 1771 2028
Size: 800 339 632 257
Fragments arranged by size:
800 632 339 257
EcoO109I rG'GnC_Cy
Cuts at: 0 6 1689 2028
Size: 6 1683 339
Fragments arranged by size:
1683 339 6
EcoRI G'AATT_C
Cuts at: 0 2022 2028
Size: 2022 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1102 2028
Size: 1102 926
HaeII r_GCGC'y
Cuts at: 0 21 31 2028
Size: 21 10 1997
Fragments arranged by size:
1997 21 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 598 630 689 721 724 756
Size: 158 32 408 32 59 32 3 32
Cuts at: 756 1314 1346 1612 1644 1699 1731 2028
Size: 558 32 266 32 55 32 297
Fragments arranged by size:
558 408 297 266 158 59 55 32
32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 0 308 1737 1760 2028
Size: 308 1429 23 268
Fragments arranged by size:
1429 308 268 23
HindIII A'AGCT_T
Cuts at: 0 413 2028
Size: 413 1615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1925 2028
Size: 1925 103
MscI TGG'CCA
Cuts at: 0 1846 2028
Size: 1846 182
MslI CAynn'nnrTG
Cuts at: 0 643 1523 1891 1921 2028
Size: 643 880 368 30 107
Fragments arranged by size:
880 643 368 107 30
MspA1I CmG'CkG
Cuts at: 0 643 2028
Size: 643 1385
NdeI CA'TA_TG
Cuts at: 0 993 2028
Size: 993 1035
NsiI A_TGCA'T
Cuts at: 0 175 2028
Size: 175 1853
NspI r_CATG'y
Cuts at: 0 753 1522 2028
Size: 753 769 506
Fragments arranged by size:
769 753 506
PciI A'CATG_T
Cuts at: 0 749 2028
Size: 749 1279
PpuMI rG'GwC_Cy
Cuts at: 0 6 2028
Size: 6 2022
PsiI TTA'TAA
Cuts at: 0 1490 1907 2028
Size: 1490 417 121
Fragments arranged by size:
1490 417 121
PvuII CAG'CTG
Cuts at: 0 643 2028
Size: 643 1385
SacI G_AGCT'C
Cuts at: 0 1989 2028
Size: 1989 39
SalI G'TCGA_C
Cuts at: 0 1758 2028
Size: 1758 270
SexAI A'CCwGG_T
Cuts at: 0 438 2028
Size: 438 1590
SfcI C'TryA_G
Cuts at: 0 693 2028
Size: 693 1335
SmlI C'TyrA_G
Cuts at: 0 1087 2028
Size: 1087 941
SspI AAT'ATT
Cuts at: 0 1539 1929 2028
Size: 1539 390 99
Fragments arranged by size:
1539 390 99
StyI C'CwwG_G
Cuts at: 0 1574 1613 2028
Size: 1574 39 415
Fragments arranged by size:
1574 415 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1 1846 2028
Size: 1 1845 182
Fragments arranged by size:
1845 182 1
TatI w'GTAC_w
Cuts at: 0 1938 2028
Size: 1938 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 105 137 2028
Size: 105 32 1891
Fragments arranged by size:
1891 105 32
XbaI T'CTAG_A
Cuts at: 0 1335 2028
Size: 1335 693
XmnI GAAnn'nnTTC
Cuts at: 0 414 814 1397 1796 2028
Size: 414 400 583 399 232
Fragments arranged by size:
583 414 400 399 232
Enzymes that do cut:
AccI AflII AflIII AleI ApoI AseI BanII BbsI
BbvCI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaWI BsaXI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BsrDI BsrGI BstXI BstYI
BstZ17I BtgI BtsI ClaI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NdeI NsiI
NspI PciI PpuMI PsiI PvuII SacI SalI SexAI
SfcI SmlI SspI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BclI BglII
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsiEI
BsiWI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII Bsu36I BtgZI CspCI DrdI EagI EcoNI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI ZraI