(Linear) (Six-base) MAPSORT of: KIEE2247.seq Check: 9374 from: 1 to: 2676 KIEE2247.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1107 2018 2676 Size: 1107 911 658 Fragments arranged by size: 1107 911 658 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 416 2676 Size: 208 208 2260 Fragments arranged by size: 2260 208 208 AflIII A'CryG_T Cuts at: 0 722 2636 2676 Size: 722 1914 40 Fragments arranged by size: 1914 722 40 AhdI GACnn_n'nnGTC Cuts at: 0 2445 2637 2676 Size: 2445 192 39 Fragments arranged by size: 2445 192 39 AleI CACnn'nnGTG Cuts at: 0 2138 2676 Size: 2138 538 AlwNI CAG_nnn'CTG Cuts at: 0 936 1638 2205 2676 Size: 936 702 567 471 Fragments arranged by size: 936 702 567 471 ApaI G_GGCC'C Cuts at: 0 1464 1931 2676 Size: 1464 467 745 Fragments arranged by size: 1464 745 467 ApaLI G'TGCA_C Cuts at: 0 259 1612 2676 Size: 259 1353 1064 Fragments arranged by size: 1353 1064 259 ApoI r'AATT_y Cuts at: 0 2371 2676 Size: 2371 305 AvaI C'yCGr_G Cuts at: 0 562 904 2210 2302 2676 Size: 562 342 1306 92 374 Fragments arranged by size: 1306 562 374 342 92 BamHI G'GATC_C Cuts at: 0 866 1934 2676 Size: 866 1068 742 Fragments arranged by size: 1068 866 742 BanI G'GyrC_C Cuts at: 0 900 959 1762 1830 2676 Size: 900 59 803 68 846 Fragments arranged by size: 900 846 803 68 59 BanII G_rGCy'C Cuts at: 0 756 1382 1464 1911 1931 2676 Size: 756 626 82 447 20 745 Fragments arranged by size: 756 745 626 447 82 20 BbeI G_GCGC'C Cuts at: 0 963 2676 Size: 963 1713 BbsI GAAGACnn'nnnn_ Cuts at: 0 680 836 2676 Size: 680 156 1840 Fragments arranged by size: 1840 680 156 BclI T'GATC_A Cuts at: 0 2055 2676 Size: 2055 621 BfrBI ATG'CAT Cuts at: 0 918 1980 2676 Size: 918 1062 696 Fragments arranged by size: 1062 918 696 BglI GCCn_nnn'nGGC Cuts at: 0 760 1549 2676 Size: 760 789 1127 Fragments arranged by size: 1127 789 760 BglII A'GATC_T Cuts at: 0 266 1433 2676 Size: 266 1167 1243 Fragments arranged by size: 1243 1167 266 BlpI GC'TnA_GC Cuts at: 0 1039 2002 2676 Size: 1039 963 674 Fragments arranged by size: 1039 963 674 Bme1580I G_kGCm'C Cuts at: 0 263 905 1464 1616 1765 1931 2676 Size: 263 642 559 152 149 166 745 Fragments arranged by size: 745 642 559 263 166 152 149 BmgBI CAC'GTC Cuts at: 0 630 1749 2676 Size: 630 1119 927 Fragments arranged by size: 1119 927 630 BmrI ACTGGGnnnn_n' Cuts at: 0 63 1403 2305 2676 Size: 63 1340 902 371 Fragments arranged by size: 1340 902 371 63 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 231 2676 Size: 231 2445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 770 910 958 1021 1132 1186 1510 Size: 226 544 140 48 63 111 54 324 Cuts at: 1510 1531 2011 2083 2676 Size: 21 480 72 593 Fragments arranged by size: 593 544 480 324 226 140 111 72 63 54 48 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1285 2676 Size: 1285 1391 BsaI GGTCTCn'nnnn_ Cuts at: 0 1086 1261 1507 2676 Size: 1086 175 246 1169 Fragments arranged by size: 1169 1086 246 175 BsaAI yAC'GTr Cuts at: 0 723 1818 2676 Size: 723 1095 858 Fragments arranged by size: 1095 858 723 BsaHI Gr'CG_yC Cuts at: 0 311 960 2676 Size: 311 649 1716 Fragments arranged by size: 1716 649 311 BsaWI w'CCGG_w Cuts at: 0 134 696 1157 2676 Size: 134 562 461 1519 Fragments arranged by size: 1519 562 461 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 253 283 2676 Size: 253 30 2393 Fragments arranged by size: 2393 253 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1150 1639 1873 1966 2320 2676 Size: 1150 489 234 93 354 356 Fragments arranged by size: 1150 489 356 354 234 93 BseYI C'CCAG_C Cuts at: 0 2147 2509 2676 Size: 2147 362 167 Fragments arranged by size: 2147 362 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 949 1003 1538 2676 Size: 949 54 535 1138 Fragments arranged by size: 1138 949 535 54 BsiEI CG_ry'CG Cuts at: 0 697 2676 Size: 697 1979 BsiHKAI G_wGCw'C Cuts at: 0 263 1382 1616 2135 2628 2676 Size: 263 1119 234 519 493 48 Fragments arranged by size: 1119 519 493 263 234 48 Bsp1286I G_dGCh'C Cuts at: 0 263 756 905 1382 1464 1616 1765 1911 Size: 263 493 149 477 82 152 149 146 Cuts at: 1911 1931 2135 2628 2676 Size: 20 204 493 48 Fragments arranged by size: 493 493 477 263 204 152 149 149 146 82 48 20 BspEI T'CCGG_A Cuts at: 0 134 1157 2676 Size: 134 1023 1519 Fragments arranged by size: 1519 1023 134 BspHI T'CATG_A Cuts at: 0 713 2384 2676 Size: 713 1671 292 Fragments arranged by size: 1671 713 292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 1107 1601 2018 2676 Size: 74 1033 494 417 658 Fragments arranged by size: 1033 658 494 417 74 BsrBI CCG'CTC Cuts at: 0 1494 2676 Size: 1494 1182 BsrFI r'CCGG_y Cuts at: 0 557 956 1046 2602 2676 Size: 557 399 90 1556 74 Fragments arranged by size: 1556 557 399 90 74 BssSI C'ACGA_G Cuts at: 0 1216 2676 Size: 1216 1460 BstAPI GCAn_nnn'nTGC Cuts at: 0 924 1830 2374 2676 Size: 924 906 544 302 Fragments arranged by size: 924 906 544 302 BstXI CCAn_nnnn'nTGG Cuts at: 0 663 2409 2676 Size: 663 1746 267 Fragments arranged by size: 1746 663 267 BstYI r'GATC_y Cuts at: 0 266 866 1154 1433 1628 1934 1964 2201 Size: 266 600 288 279 195 306 30 237 Cuts at: 2201 2501 2676 Size: 300 175 Fragments arranged by size: 600 306 300 288 279 266 237 195 175 30 Bsu36I CC'TnA_GG Cuts at: 0 1790 2676 Size: 1790 886 BtgI C'CryG_G Cuts at: 0 419 883 1583 2126 2531 2648 2676 Size: 419 464 700 543 405 117 28 Fragments arranged by size: 700 543 464 419 405 117 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1353 2154 2565 2676 Size: 1353 801 411 111 Fragments arranged by size: 1353 801 411 111 DraIII CAC_nnn'GTG Cuts at: 0 1411 2676 Size: 1411 1265 DrdI GACnn_nn'nnGTC Cuts at: 0 723 2676 Size: 723 1953 EaeI y'GGCC_r Cuts at: 0 230 797 845 1697 2676 Size: 230 567 48 852 979 Fragments arranged by size: 979 852 567 230 48 EarI CTCTTCn'nnn_ Cuts at: 0 41 161 419 2020 2676 Size: 41 120 258 1601 656 Fragments arranged by size: 1601 656 258 120 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 778 859 2676 Size: 778 81 1817 Fragments arranged by size: 1817 778 81 EcoICRI GAG'CTC Cuts at: 0 1380 2676 Size: 1380 1296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 226 416 770 910 958 1021 1132 Size: 208 18 190 354 140 48 63 111 Cuts at: 1132 1186 1510 1531 2011 2083 2676 Size: 54 324 21 480 72 593 Fragments arranged by size: 593 480 354 324 208 190 140 111 72 63 54 48 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 136 1114 2676 Size: 136 978 1562 Fragments arranged by size: 1562 978 136 EcoO109I rG'GnC_Cy Cuts at: 0 63 285 1293 1460 1461 1590 1928 1985 Size: 63 222 1008 167 1 129 338 57 Cuts at: 1985 2299 2676 Size: 314 377 Fragments arranged by size: 1008 377 338 314 222 167 129 63 57 1 HaeII r_GCGC'y Cuts at: 0 963 1695 2095 2656 2676 Size: 963 732 400 561 20 Fragments arranged by size: 963 732 561 400 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2431 2463 2623 2655 2676 Size: 2431 32 160 32 21 Fragments arranged by size: 2431 160 32 32 21 HincII GTy'rAC Cuts at: 0 279 492 804 1416 2037 2676 Size: 279 213 312 612 621 639 Fragments arranged by size: 639 621 612 312 279 213 KasI G'GCGC_C Cuts at: 0 959 2676 Size: 959 1717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 296 663 839 2676 Size: 296 367 176 1837 Fragments arranged by size: 1837 367 296 176 MscI TGG'CCA Cuts at: 0 232 847 1699 2676 Size: 232 615 852 977 Fragments arranged by size: 977 852 615 232 MslI CAynn'nnrTG Cuts at: 0 418 1975 2138 2140 2530 2676 Size: 418 1557 163 2 390 146 Fragments arranged by size: 1557 418 390 163 146 2 MspA1I CmG'CkG Cuts at: 0 999 1527 1827 2061 2157 2676 Size: 999 528 300 234 96 519 Fragments arranged by size: 999 528 519 300 234 96 NarI GG'CG_CC Cuts at: 0 960 2676 Size: 960 1716 NcoI C'CATG_G Cuts at: 0 1583 2126 2531 2676 Size: 1583 543 405 145 Fragments arranged by size: 1583 543 405 145 NsiI A_TGCA'T Cuts at: 0 920 1982 2676 Size: 920 1062 694 Fragments arranged by size: 1062 920 694 NspI r_CATG'y Cuts at: 0 39 922 1867 2640 2676 Size: 39 883 945 773 36 Fragments arranged by size: 945 883 773 39 36 PasI CC'CwG_GG Cuts at: 0 369 2358 2676 Size: 369 1989 318 Fragments arranged by size: 1989 369 318 PciI A'CATG_T Cuts at: 0 2636 2676 Size: 2636 40 PflMI CCAn_nnn'nTGG Cuts at: 0 2487 2676 Size: 2487 189 PfoI T'CCnGG_A Cuts at: 0 71 176 407 947 2084 2676 Size: 71 105 231 540 1137 592 Fragments arranged by size: 1137 592 540 231 105 71 PmlI CAC'GTG Cuts at: 0 1818 2676 Size: 1818 858 PpuMI rG'GwC_Cy Cuts at: 0 63 1590 1985 2299 2676 Size: 63 1527 395 314 377 Fragments arranged by size: 1527 395 377 314 63 PspOMI G'GGCC_C Cuts at: 0 1460 1927 2676 Size: 1460 467 749 Fragments arranged by size: 1460 749 467 PstI C_TGCA'G Cuts at: 0 1025 1598 2676 Size: 1025 573 1078 Fragments arranged by size: 1078 1025 573 PvuII CAG'CTG Cuts at: 0 999 1527 1827 2061 2157 2676 Size: 999 528 300 234 96 519 Fragments arranged by size: 999 528 519 300 234 96 RsrII CG'GwC_CG Cuts at: 0 1496 2676 Size: 1496 1180 SacI G_AGCT'C Cuts at: 0 1382 2676 Size: 1382 1294 SanDI GG'GwC_CC Cuts at: 0 1985 2299 2676 Size: 1985 314 377 Fragments arranged by size: 1985 377 314 SapI GCTCTTCn'nnn_ Cuts at: 0 2020 2676 Size: 2020 656 SbfI CC_TGCA'GG Cuts at: 0 1598 2676 Size: 1598 1078 ScaI AGT'ACT Cuts at: 0 2575 2676 Size: 2575 101 SfcI C'TryA_G Cuts at: 0 1021 1594 2676 Size: 1021 573 1082 Fragments arranged by size: 1082 1021 573 SfoI GGC'GCC Cuts at: 0 961 2676 Size: 961 1715 SmaI CCC'GGG Cuts at: 0 2212 2676 Size: 2212 464 SmlI C'TyrA_G Cuts at: 0 1300 2676 Size: 1300 1376 SphI G_CATG'C Cuts at: 0 922 1867 2676 Size: 922 945 809 Fragments arranged by size: 945 922 809 StuI AGG'CCT Cuts at: 0 1207 1222 1315 2431 2676 Size: 1207 15 93 1116 245 Fragments arranged by size: 1207 1116 245 93 15 StyI C'CwwG_G Cuts at: 0 437 977 1583 2126 2219 2531 2676 Size: 437 540 606 543 93 312 145 Fragments arranged by size: 606 543 540 437 312 145 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1422 1513 2676 Size: 1422 91 1163 Fragments arranged by size: 1422 1163 91 TatI w'GTAC_w Cuts at: 0 1529 2573 2676 Size: 1529 1044 103 Fragments arranged by size: 1529 1044 103 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 253 285 2283 2315 2676 Size: 253 32 1998 32 361 Fragments arranged by size: 1998 361 253 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2482 2676 Size: 2482 194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 50 745 855 2124 2676 Size: 50 695 110 1269 552 Fragments arranged by size: 1269 695 552 110 50 XmaI C'CCGG_G Cuts at: 0 2210 2676 Size: 2210 466 XmnI GAAnn'nnTTC Cuts at: 0 2248 2676 Size: 2248 428 Enzymes that do cut: AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacI SanDI SapI SbfI ScaI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BmtI Bpu10I BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtsI ClaI CspCI DraI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI SacII SalI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI