################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbvCI BfrI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 299 GGTACC G^GTACC AccI 1364 GTCTAC GT^[AC][GT]AC AccII 1036 CGCG CG^CG AflIII 1368 ACACGT A^C[GA][CT]GT Alw44I 741 GTGCAC G^TGCAC AlwI 1138 GGATC GGATC(4/5) ApaLI 741 GTGCAC G^TGCAC Asp718I 299 GGTACC G^GTACC AspI 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1369 CACGTG CAC^GTG BbsI 160 GAAGAC GAAGAC(2/6) BcgI 18 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 980 GTATCC GTATCC(6/5) BclI 2 TGATCA T^GATCA BfaI 721 CTAG C^TAG BlpI 614 GCTCAGC GC^T[ACGT]AGC Bpu1102I 614 GCTCAGC GC^T[ACGT]AGC BpuAI 160 GAAGAC GAAGAC(2/6) BsaAI 1369 CACGTG [CT]AC^GT[GA] BsaI 1299 GAGACC GGTCTC(1/5) BspDI 1073 ATCGAT AT^CGAT BssSI 957 CTCGTG CACGAG(-5/-1) BstUI 1036 CGCG CG^CG CelII 614 GCTCAGC GC^T[ACGT]AGC ClaI 1073 ATCGAT AT^CGAT EarI 1396 GAAGAG CTCTTC(1/4) EcoRV 1096 GATATC GAT^ATC FbaI 2 TGATCA T^GATCA KpnI 299 GGTACC GGTAC^C Ksp632I 1396 GAAGAG CTCTTC(1/4) MaeI 721 CTAG C^TAG MvnI 1036 CGCG CG^CG NheI 720 GCTAGC G^CTAGC NspI 1172 GCATGC [GA]CATG^[CT] PflFI 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 955 GACTC GAGTC(4/5) PmaCI 1369 CACGTG CAC^GTG PmlI 1369 CACGTG CAC^GTG PshAI 163 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1047 CTGCAG CTGCA^G SbfI 1046 CCTGCAGG CCTGCA^GG SphI 1172 GCATGC GCATG^C Sse8387I 1046 CCTGCAGG CCTGCA^GG Tth111I 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 764 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1333 CAGAAGCTG AocI 646 CCTAAGG CC^T[ACGT]AGG 1390 CCTGAGG AvaI 714 CCCGGG C^[CT]CG[GA]G 1020 CCCGGG BanI 299 GGTACC G^G[CT][GA]CC 345 GGCACC BpmI 370 CTCCAG CTGGAG(16/14) 358 CTGGAG BseRI 1318 GAGGAG GAGGAG(10/8) 1348 GAGGAG BsgI 674 CTGCAC GTGCAG(16/14) 1218 CTGCAC BsoBI 714 CCCGGG C^[CT]CG[GA]G 1020 CCCGGG BsrFI 302 ACCGGC [GA]^CCGG[CT] 745 ACCGGC BsrI 102 CCAGT ACTGG(1/-1) 763 CCAGT BstF5I 1116 CATCC GGATG(2/0) 942 GGATG Bsu36I 646 CCTAAGG CC^T[ACGT]AGG 1390 CCTGAGG Cfr10I 302 ACCGGC [GA]^CCGG[CT] 745 ACCGGC DpnII 3 GATC ^GATC 1139 GATC DraIII 856 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1310 CACGGTGTG Eco81I 646 CCTAAGG CC^T[ACGT]AGG 1390 CCTGAGG FokI 1116 CATCC GGATG(9/13) 942 GGATG HaeII 305 GGCGCT [GA]GCGC^[CT] 1211 AGCGCC MboI 3 GATC ^GATC 1139 GATC MslI 921 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1072 CATCGATGTG NcoI 833 CCATGG C^CATGG 1256 CCATGG NdeII 3 GATC ^GATC 1139 GATC PflMI 763 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 828 CCAGACCATGG Sau3AI 3 GATC ^GATC 1139 GATC SfaNI 895 GATGC GCATC(5/9) 1115 GCATC SfcI 1047 CTGCAG C^T[GA][CT]AG 1110 CTACAG SmaI 714 CCCGGG CCC^GGG 1020 CCCGGG StuI 374 AGGCCT AGG^CCT 545 AGGCCT TfiI 112 GAATC G^A[AT]TC 538 GAATC Van91I 763 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 828 CCAGACCATGG XmaCI 714 CCCGGG C^CCGGG 1020 CCCGGG XmaI 714 CCCGGG C^CCGGG 1020 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI ApaI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PpuMI PvuII RsaI Sau96I ScrFI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI