Restriction map for
pFN21AE1349
(Circular) (Six-base) MAPSORT of: pFN21AE1349.seq Check: 3856 from: 1 to: 6112
pFN21AE1349 6112 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 2465
Size: 6112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3370 3455 1937
Size: 1433 85 4594
Fragments arranged by size:
4594 1433 85
Acc65I G'GTAC_C
Cuts at: 2304 3433 2304
Size: 1129 4983
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4175 5559 5827 1632
Size: 2543 1384 268 1917
Fragments arranged by size:
2543 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 3373 5011 5988 1203
Size: 2170 1638 977 1327
Fragments arranged by size:
2170 1638 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 2930 1166
Size: 1764 4348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2771 3248 1184
Size: 1587 477 4048
Fragments arranged by size:
4048 1587 477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 6080
AlwNI CAG_nnn'CTG
Cuts at: 2774 3343 3855 5427 2774
Size: 569 512 1572 3459
Fragments arranged by size:
3459 1572 569 512
ApaI G_GGCC'C
Cuts at: 2266 2436 2606 2798 2885 2266
Size: 170 170 192 87 5493
Fragments arranged by size:
5493 192 170 170 87
ApaLI G'TGCA_C
Cuts at: 2746 4169 5325 2746
Size: 1423 1156 3533
Fragments arranged by size:
3533 1423 1156
ApoI r'AATT_y
Cuts at: 1493 3421 3647 3799 5908 1493
Size: 1928 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2719 3025 3437 1870
Size: 73 776 306 412 4545
Fragments arranged by size:
4545 776 412 306 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 2350 3433 4888 618
Size: 325 217 627 517 46 1083 1455 1842
Fragments arranged by size:
1842 1455 1083 627 517 325 217 46
BanII G_rGCy'C
Cuts at: 1391 2217 2266 2294 2436 2606 2691 2798 2807
Size: 826 49 28 142 170 85 107 9
Cuts at: 2807 2885 2981 3431 1391
Size: 78 96 450 4072
Fragments arranged by size:
4072 826 450 170 142 107 96 85
78 49 28 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 928
Size: 971 273 4868
Fragments arranged by size:
4868 971 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2012 2046 4303 4337 1903
Size: 34 75 34 2257 34 3678
Fragments arranged by size:
3678 2257 75 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2996 4004 5214 2996
Size: 1008 1210 3894
Fragments arranged by size:
3894 1210 1008
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 2620 3529 2620
Size: 909 5203
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2436 2606 2750 2798 2871 2885 2937
Size: 476 170 170 144 48 73 14 52
Cuts at: 2937 3211 4173 5329 1790
Size: 274 962 1156 2573
Fragments arranged by size:
2573 1156 962 476 274 170 170 144
73 52 48 14
BmgBI CAC'GTC
Cuts at: 1318 2280 2853 1318
Size: 962 573 4577
Fragments arranged by size:
4577 962 573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2762 3843
Size: 416 53 83 399 24 684 661 1081
Cuts at: 3843 4801 442
Size: 958 1753
Fragments arranged by size:
1753 1081 958 684 661 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2729 1056
Size: 1673 4439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2977 2464
Size: 513 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2384 4772 1239
Size: 1121 24 2388 2579
Fragments arranged by size:
2579 2388 1121 24
Bpu10I CC'TnA_GC
Cuts at: 2685 2685
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3298 4775 882
Size: 617 1799 1477 2219
Fragments arranged by size:
2219 1799 1477 617
BsaAI yAC'GTr
Cuts at: 493 1204 3376 3903 493
Size: 711 2172 527 2702
Fragments arranged by size:
2702 2172 711 527
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2968 2998 3219 3249 1178
Size: 30 165 30 1565 30 221 30 4041
Fragments arranged by size:
4041 1565 221 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3338 3368 1186
Size: 2152 30 3930
Fragments arranged by size:
3930 2152 30
BseYI C'CCAG_C
Cuts at: 2295 2427 2562 2774 2781 2871 5315 5811 2295
Size: 132 135 212 7 90 2444 496 2596
Fragments arranged by size:
2596 2444 496 212 135 132 90 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2664 3208 1163
Size: 573 928 544 4067
Fragments arranged by size:
4067 928 573 544
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2285 2339 2750 4173 4258 5329 2097
Size: 188 54 411 1423 85 1071 2880
Fragments arranged by size:
2880 1423 1071 411 188 85 54
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2217 2266 2285 2294 2339 2436
Size: 399 307 120 49 19 9 45 97
Cuts at: 2436 2606 2691 2750 2798 2807 2871 2885 2937
Size: 170 85 59 48 9 64 14 52
Cuts at: 2937 2981 3211 3431 4173 4258 5329 1391
Size: 44 230 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 307 230 220 170
120 97 85 85 64 59 52 49
48 45 44 19 14 9 9
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2465 2557 3059 3467 844
Size: 1376 245 92 502 408 3489
Fragments arranged by size:
3489 1376 502 408 245 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2307 2750 4756 4943 1700
Size: 110 497 443 2006 187 2869
Fragments arranged by size:
2869 2006 497 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 2962 4172 5184 2962
Size: 1210 1012 3890
Fragments arranged by size:
3890 1210 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2652 3396 2652
Size: 744 5368
BtgI C'CryG_G
Cuts at: 513 1065 2632 2838 3261 3314 513
Size: 552 1567 206 423 53 3311
Fragments arranged by size:
3311 1567 552 423 206 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2913 487
Size: 683 56 1687 3686
Fragments arranged by size:
3686 1687 683 56
BtsI GCAGTG_nn'
Cuts at: 2181 2339 2402 2521 3109 3127 3628 4422 4442
Size: 158 63 119 588 18 501 794 20
Cuts at: 4442 2181
Size: 3851
Fragments arranged by size:
3851 794 588 501 158 119 63 20
18
ClaI AT'CG_AT
Cuts at: 1554 3079 1554
Size: 1525 4587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DraIII CAC_nnn'GTG
Cuts at: 2866 3320 2866
Size: 454 5658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3559 4448 6077
Size: 54 1425 50 275 18 1729 889 1629
Cuts at: 6077 8
Size: 43
Fragments arranged by size:
1729 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3396 4042 1353
Size: 2043 646 3423
Fragments arranged by size:
3423 2043 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2384 4175 4772 5559 5827 1239
Size: 393 728 24 1791 597 787 268 1524
Fragments arranged by size:
1791 1524 787 728 597 393 268 24
EcoO109I rG'GnC_Cy
Cuts at: 2026 2137 2232 2263 2358 2432 2433 2469 2586
Size: 111 95 31 95 74 1 36 117
Cuts at: 2586 2602 2603 2786 2794 2795 2881 2882 3048
Size: 16 1 183 8 1 86 1 166
Cuts at: 3048 2026
Size: 5090
Fragments arranged by size:
5090 183 166 117 111 95 95 86
74 36 31 16 8 1 1 1
1
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
EcoRV GAT'ATC
Cuts at: 3103 3103
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2314 3220 5259 1678
Size: 636 906 2039 2531
Fragments arranged by size:
2531 2039 906 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2842 2874 4753 4785 4827
Size: 32 135 32 1270 32 1879 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 1879 1270 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 2308 3437 2308
Size: 1129 4983
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3013 5226 5410 1305
Size: 1708 2213 184 2007
Fragments arranged by size:
2213 2007 1708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2930 3081 4070 4429 4588 518
Size: 648 1764 151 989 359 159 2042
Fragments arranged by size:
2042 1764 989 648 359 159 151
MspA1I CmG'CkG
Cuts at: 1606 2050 2299 2740 2930 3012 3310 3528 4205
Size: 444 249 441 190 82 298 218 677
Cuts at: 4205 5353 5598 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 441 298 249 245
218 190 82
NcoI C'CATG_G
Cuts at: 513 1065 2838 3261 513
Size: 552 1773 423 3364
Fragments arranged by size:
3364 1773 552 423
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 2725 1052
Size: 1673 4439
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 3181 3470 5015 1132
Size: 97 1952 289 1545 2229
Fragments arranged by size:
2229 1952 1545 289 97
PasI CC'CwG_GG
Cuts at: 1348 2436 1348
Size: 1088 5024
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2774 2839 1108
Size: 1666 65 4381
Fragments arranged by size:
4381 1666 65
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PmlI CAC'GTG
Cuts at: 3376 3376
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 2358 2469 3048 2232
Size: 126 111 579 5296
Fragments arranged by size:
5296 579 126 111
PshAI GACnn'nnGTC
Cuts at: 1613 2172 1613
Size: 559 5553
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 2262 2432 2602 2794 2881 2262
Size: 170 170 192 87 5493
Fragments arranged by size:
5493 192 170 170 87
PstI C_TGCA'G
Cuts at: 830 3056 3464 830
Size: 2226 408 3478
Fragments arranged by size:
3478 2226 408
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2299 2930 3012 2299
Size: 631 82 5399
Fragments arranged by size:
5399 631 82
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SanDI GG'GwC_CC
Cuts at: 2358 2469 2358
Size: 111 6001
SbfI CC_TGCA'GG
Cuts at: 3056 3464 3056
Size: 408 5704
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 3052 3115 3460 4595 5276 5467 826
Size: 219 2007 63 345 1135 681 191 1471
Fragments arranged by size:
2007 1471 1135 681 345 219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 2721 3027 3439 1872
Size: 849 306 412 4545
Fragments arranged by size:
4545 849 412 306
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4216 5117 5379 820
Size: 197 926 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3181 3470 1132
Size: 2049 289 3774
Fragments arranged by size:
3774 2049 289
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 2552 1517
Size: 864 171 5077
Fragments arranged by size:
5077 864 171
StyI C'CwwG_G
Cuts at: 513 1065 2789 2838 3261 3551 513
Size: 552 1724 49 423 290 3074
Fragments arranged by size:
3074 1724 552 423 290 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2576 4139 4156 4309 4494 4939
Size: 1255 540 456 1563 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1563 1498 1255 540 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 2939 2971 3219 3251 6072 6104 2381
Size: 32 526 32 248 32 2821 32 2389
Fragments arranged by size:
2821 2389 526 248 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3366 1316
Size: 2050 4062
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2702 3259 2204
Size: 498 557 5057
Fragments arranged by size:
5057 557 498
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 2719 3025 3437 1870
Size: 849 306 412 4545
Fragments arranged by size:
4545 849 412 306
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI