Restriction map for
pFN21AE1349
(Linear) (Six-base) MAPSORT of: KIEE1349.seq Check: 5370 from: 1 to: 1407
KIEE1349.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 1407
Size: 460 947
AccI GT'mk_AC
Cuts at: 0 1365 1407
Size: 1365 42
Acc65I G'GTAC_C
Cuts at: 0 299 1407
Size: 299 1108
AflIII A'CryG_T
Cuts at: 0 1368 1407
Size: 1368 39
AleI CACnn'nnGTG
Cuts at: 0 925 1407
Size: 925 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 766 1243 1407
Size: 766 477 164
Fragments arranged by size:
766 477 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1407
Size: 982 32 393
Fragments arranged by size:
982 393 32
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1338 1407
Size: 769 569 69
Fragments arranged by size:
769 569 69
ApaI G_GGCC'C
Cuts at: 0 261 431 601 793 880 1407
Size: 261 170 170 192 87 527
Fragments arranged by size:
527 261 192 170 170 87
ApaLI G'TGCA_C
Cuts at: 0 741 1407
Size: 741 666
AvaI C'yCGr_G
Cuts at: 0 714 1020 1407
Size: 714 306 387
Fragments arranged by size:
714 387 306
BanI G'GyrC_C
Cuts at: 0 299 345 1407
Size: 299 46 1062
Fragments arranged by size:
1062 299 46
BanII G_rGCy'C
Cuts at: 0 212 261 289 431 601 686 793 802
Size: 212 49 28 142 170 85 107 9
Cuts at: 802 880 976 1407
Size: 78 96 431
Fragments arranged by size:
431 212 170 142 107 96 85 78
49 28 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 1407
Size: 167 1240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7 41 1407
Size: 7 34 1366
Fragments arranged by size:
1366 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 991 1407
Size: 991 416
BclI T'GATC_A
Cuts at: 0 2 1407
Size: 2 1405
BlpI GC'TnA_GC
Cuts at: 0 615 1407
Size: 615 792
Bme1580I G_kGCm'C
Cuts at: 0 261 431 601 745 793 866 880 932
Size: 261 170 170 144 48 73 14 52
Cuts at: 932 1206 1407
Size: 274 201
Fragments arranged by size:
274 261 201 170 170 144 73 52
48 14
BmgBI CAC'GTC
Cuts at: 0 275 848 1407
Size: 275 573 559
Fragments arranged by size:
573 559 275
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 757 1407
Size: 96 661 650
Fragments arranged by size:
661 650 96
BmtI G_CTAG'C
Cuts at: 0 724 1407
Size: 724 683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 972 1407
Size: 459 513 435
Fragments arranged by size:
513 459 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 379 1407
Size: 355 24 1028
Fragments arranged by size:
1028 355 24
Bpu10I CC'TnA_GC
Cuts at: 0 680 1407
Size: 680 727
BsaI GGTCTCn'nnnn_
Cuts at: 0 1293 1407
Size: 1293 114
BsaAI yAC'GTr
Cuts at: 0 1371 1407
Size: 1371 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 963 993 1214 1244 1407
Size: 963 30 221 30 163
Fragments arranged by size:
963 221 163 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1333 1363 1407
Size: 1333 30 44
Fragments arranged by size:
1333 44 30
BseYI C'CCAG_C
Cuts at: 0 290 422 557 769 776 866 1407
Size: 290 132 135 212 7 90 541
Fragments arranged by size:
541 290 212 135 132 90 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1203 1407
Size: 659 544 204
Fragments arranged by size:
659 544 204
BsiHKAI G_wGCw'C
Cuts at: 0 92 280 334 745 1407
Size: 92 188 54 411 662
Fragments arranged by size:
662 411 188 92 54
Bsp1286I G_dGCh'C
Cuts at: 0 92 212 261 280 289 334 431 601
Size: 92 120 49 19 9 45 97 170
Cuts at: 601 686 745 793 802 866 880 932 976
Size: 85 59 48 9 64 14 52 44
Cuts at: 976 1206 1407
Size: 230 201
Fragments arranged by size:
230 201 170 120 97 92 85 64
59 52 49 48 45 44 19 14
9 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 460 552 1054 1407
Size: 215 245 92 502 353
Fragments arranged by size:
502 353 245 215 92
BsrFI r'CCGG_y
Cuts at: 0 302 745 1407
Size: 302 443 662
Fragments arranged by size:
662 443 302
BssSI C'ACGA_G
Cuts at: 0 957 1407
Size: 957 450
Bsu36I CC'TnA_GG
Cuts at: 0 647 1391 1407
Size: 647 744 16
Fragments arranged by size:
744 647 16
BtgI C'CryG_G
Cuts at: 0 627 833 1256 1309 1407
Size: 627 206 423 53 98
Fragments arranged by size:
627 423 206 98 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 908 1407
Size: 908 499
BtsI GCAGTG_nn'
Cuts at: 0 176 334 397 516 1104 1122 1407
Size: 176 158 63 119 588 18 285
Fragments arranged by size:
588 285 176 158 119 63 18
ClaI AT'CG_AT
Cuts at: 0 1074 1407
Size: 1074 333
DraIII CAC_nnn'GTG
Cuts at: 0 861 1315 1407
Size: 861 454 92
Fragments arranged by size:
861 454 92
EarI CTCTTCn'nnn_
Cuts at: 0 1391 1407
Size: 1391 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 379 1407
Size: 355 24 1028
Fragments arranged by size:
1028 355 24
EcoO109I rG'GnC_Cy
Cuts at: 0 21 132 227 258 353 427 428 464
Size: 21 111 95 31 95 74 1 36
Cuts at: 464 581 597 598 781 789 790 876 877
Size: 117 16 1 183 8 1 86 1
Cuts at: 877 1043 1407
Size: 166 364
Fragments arranged by size:
364 183 166 117 111 95 95 86
74 36 31 21 16 8 1 1
1 1
EcoRV GAT'ATC
Cuts at: 0 1098 1407
Size: 1098 309
HaeII r_GCGC'y
Cuts at: 0 309 1215 1407
Size: 309 906 192
Fragments arranged by size:
906 309 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 837 869 1407
Size: 837 32 538
Fragments arranged by size:
837 538 32
KpnI G_GTAC'C
Cuts at: 0 303 1407
Size: 303 1104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1407
Size: 1008 399
MslI CAynn'nnrTG
Cuts at: 0 925 1076 1407
Size: 925 151 331
Fragments arranged by size:
925 331 151
MspA1I CmG'CkG
Cuts at: 0 45 294 735 925 1007 1305 1407
Size: 45 249 441 190 82 298 102
Fragments arranged by size:
441 298 249 190 102 82 45
NcoI C'CATG_G
Cuts at: 0 833 1256 1407
Size: 833 423 151
Fragments arranged by size:
833 423 151
NheI G'CTAG_C
Cuts at: 0 720 1407
Size: 720 687
NspI r_CATG'y
Cuts at: 0 1176 1407
Size: 1176 231
PasI CC'CwG_GG
Cuts at: 0 431 1407
Size: 431 976
PflMI CCAn_nnn'nTGG
Cuts at: 0 769 834 1407
Size: 769 65 573
Fragments arranged by size:
769 573 65
PmlI CAC'GTG
Cuts at: 0 1371 1407
Size: 1371 36
PpuMI rG'GwC_Cy
Cuts at: 0 227 353 464 1043 1407
Size: 227 126 111 579 364
Fragments arranged by size:
579 364 227 126 111
PshAI GACnn'nnGTC
Cuts at: 0 167 1407
Size: 167 1240
PspOMI G'GGCC_C
Cuts at: 0 257 427 597 789 876 1407
Size: 257 170 170 192 87 531
Fragments arranged by size:
531 257 192 170 170 87
PstI C_TGCA'G
Cuts at: 0 1051 1407
Size: 1051 356
PvuII CAG'CTG
Cuts at: 0 294 925 1007 1407
Size: 294 631 82 400
Fragments arranged by size:
631 400 294 82
SanDI GG'GwC_CC
Cuts at: 0 353 464 1407
Size: 353 111 943
Fragments arranged by size:
943 353 111
SbfI CC_TGCA'GG
Cuts at: 0 1051 1407
Size: 1051 356
SfcI C'TryA_G
Cuts at: 0 1047 1110 1407
Size: 1047 63 297
Fragments arranged by size:
1047 297 63
SmaI CCC'GGG
Cuts at: 0 716 1022 1407
Size: 716 306 385
Fragments arranged by size:
716 385 306
SphI G_CATG'C
Cuts at: 0 1176 1407
Size: 1176 231
StuI AGG'CCT
Cuts at: 0 376 547 1407
Size: 376 171 860
Fragments arranged by size:
860 376 171
StyI C'CwwG_G
Cuts at: 0 784 833 1256 1407
Size: 784 49 423 151
Fragments arranged by size:
784 423 151 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 571 1407
Size: 115 456 836
Fragments arranged by size:
836 456 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 934 966 1214 1246 1407
Size: 376 32 526 32 248 32 161
Fragments arranged by size:
526 376 248 161 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1361 1407
Size: 1361 46
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 199 697 1254 1407
Size: 199 498 557 153
Fragments arranged by size:
557 498 199 153
XmaI C'CCGG_G
Cuts at: 0 714 1020 1407
Size: 714 306 387
Fragments arranged by size:
714 387 306
Enzymes that do cut:
AarI AccI Acc65I AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BcgI BciVI
BclI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrFI BssSI Bsu36I BtgI BtgZI BtsI
ClaI DraIII EarI Eco57MI EcoO109I EcoRV HaeII Hin4I
KpnI MmeI MslI MspA1I NcoI NheI NspI PasI
PflMI PmlI PpuMI PshAI PspOMI PstI PvuII SanDI
SbfI SfcI SmaI SphI StuI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BfrBI BglI BglII BpuEI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PciI PfoI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TatI XbaI XhoI XmnI
ZraI