################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 528 CGCG CG^CG AflIII 306 ACACGT A^C[GA][CT]GT ApaI 663 GGGCCC GGGCC^C AvaI 362 CCCGAG C^[CT]CG[GA]G BalI 1115 TGGCCA TGG^CCA BamHI 1702 GGATCC G^GATCC BanI 1078 GGCACC G^G[CT][GA]CC BfaI 935 CTAG C^TAG BglI 595 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsoBI 362 CCCGAG C^[CT]CG[GA]G BspHI 149 TCATGA T^CATGA BspMI 1040 ACCTGC ACCTGC(4/8) Bst1107I 787 GTATAC GTA^TAC BstEII 1665 GGTGACC G^GT[ACGT]ACC BstPI 1665 GGTGACC G^GT[ACGT]ACC BstUI 528 CGCG CG^CG BstXI 1175 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1702 GGATCC [GA]^GATC[CT] BstZ17I 787 GTATAC GTA^TAC DsaI 1118 CCATGG C^C[GA][CT]GG EaeI 1115 TGGCCA [CT]^GGCC[GA] EcoO65I 1665 GGTGACC G^GT[ACGT]ACC EcoRV 178 GATATC GAT^ATC EcoT22I 43 ATGCAT ATGCA^T HgaI 1368 GCGTC GACGC(5/10) HindIII 462 AAGCTT A^AGCTT MaeI 935 CTAG C^TAG MaeII 308 ACGT A^CGT MflI 1702 GGATCC [GA]^GATC[CT] MluNI 1115 TGGCCA TGG^CCA MscI 1115 TGGCCA TGG^CCA MspA1I 804 CAGCGG C[AC]G^C[GT]G MvnI 528 CGCG CG^CG NcoI 1118 CCATGG C^CATGG NruI 527 TCGCGA TCG^CGA NsiI 43 ATGCAT ATGCA^T PshAI 322 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 149 TCATGA T^CATGA SacI 1018 GAGCTC GAGCT^C ScaI 1028 AGTACT AGT^ACT SfcI 468 CTACAG C^T[GA][CT]AG SspI 400 AATATT AAT^ATT Tsp509I 737 AATT ^AATT XhoII 1702 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 787 GTATAC GT^[AC][GT]AC 1084 GTAGAC BanII 663 GGGCCC G[GA]GC[CT]^C 1018 GAGCTC BbsI 960 GAAGAC GAAGAC(2/6) 310 GTCTTC BbvCI 1051 GCTGAGG CCTCAGC(-5/-2) 1132 GCTGAGG BlpI 604 GCTCAGC GC^T[ACGT]AGC 1020 GCTCAGC Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC 1020 GCTCAGC BpuAI 960 GAAGAC GAAGAC(2/6) 310 GTCTTC BsaI 799 GAGACC GGTCTC(1/5) 914 GGTCTC BsgI 302 CTGCAC GTGCAG(16/14) 1042 CTGCAC BsmAI 799 GAGAC GTCTC(1/5) 915 GTCTC BsrBI 199 CCGCTC CCGCTC(-3/-3) 1387 GAGCGG CelII 604 GCTCAGC GC^T[ACGT]AGC 1020 GCTCAGC EcoNI 840 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1047 CCTTGCTGAGG HaeII 509 AGCGCC [GA]GCGC^[CT] 1546 AGCGCC MslI 111 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1320 CACCAACATG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I