(Circular) (Six-base) MAPSORT of: pFN21AE4532.seq Check: 3623 from: 1 to: 6487 pFN21AE4532 6487 bp With 182 enzymes: SgfI * March 28, 2012 14:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3054 3054 Size: 6487 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2793 3090 3830 1937 Size: 856 297 740 4594 Fragments arranged by size: 4594 856 740 297 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3017 3517 3770 4550 5934 6202 1632 Size: 941 444 500 253 780 1384 268 1917 Fragments arranged by size: 1917 1384 941 780 500 444 268 253 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6290 AflIII A'CryG_T Cuts at: 1203 2311 5386 6363 1203 Size: 1108 3075 977 1327 Fragments arranged by size: 3075 1327 1108 977 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2138 1184 Size: 954 5533 AlwNI CAG_nnn'CTG Cuts at: 2695 2995 3118 3137 3736 4230 5802 2695 Size: 300 123 19 599 494 1572 3380 Fragments arranged by size: 3380 1572 599 494 300 123 19 ApaI G_GGCC'C Cuts at: 2672 2672 Size: 6487 ApaLI G'TGCA_C Cuts at: 4544 5700 4544 Size: 1156 5331 ApoI r'AATT_y Cuts at: 1493 3796 4022 4174 6283 1493 Size: 2303 226 152 2109 1697 Fragments arranged by size: 2303 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 2367 3812 1870 Size: 73 424 1445 4545 Fragments arranged by size: 4545 1445 424 73 BamHI G'GATC_C Cuts at: 3707 3817 4184 3707 Size: 110 367 6010 Fragments arranged by size: 6010 367 110 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3083 3808 5263 618 Size: 325 217 627 1296 725 1455 1842 Fragments arranged by size: 1842 1455 1296 725 627 325 217 BanII G_rGCy'C Cuts at: 1391 2672 3027 3806 1391 Size: 1281 355 779 4072 Fragments arranged by size: 4072 1281 779 355 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 2972 928 Size: 971 409 664 4443 Fragments arranged by size: 4443 971 664 409 BbvCI CC'TCA_GC Cuts at: 3057 3138 3057 Size: 81 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4678 4712 1903 Size: 34 2741 34 3678 Fragments arranged by size: 3678 2741 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4379 5589 4379 Size: 1210 5277 BclI T'GATC_A Cuts at: 1548 1548 Size: 6487 BfrBI ATG'CAT Cuts at: 2050 2050 Size: 6487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2606 5098 136 Size: 107 122 71 631 605 934 2492 1525 Fragments arranged by size: 2492 1525 934 631 605 122 107 71 BglII A'GATC_T Cuts at: 6482 6482 Size: 6487 BlpI GC'TnA_GC Cuts at: 2610 3026 3904 2610 Size: 416 878 5193 Fragments arranged by size: 5193 878 416 Bme1580I G_kGCm'C Cuts at: 1790 2672 3131 3351 3567 4548 5704 1790 Size: 882 459 220 216 981 1156 2573 Fragments arranged by size: 2573 1156 981 882 459 220 216 BmgBI CAC'GTC Cuts at: 1318 2314 1318 Size: 996 5491 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4218 5176 442 Size: 416 53 83 399 24 2801 958 1753 Fragments arranged by size: 2801 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2222 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2531 2979 5147 1239 Size: 998 294 448 2168 2579 Fragments arranged by size: 2579 2168 998 448 294 Bpu10I CC'TnA_GC Cuts at: 3057 3138 3057 Size: 81 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4612 5477 5775 4612 Size: 865 298 5324 Fragments arranged by size: 5324 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2798 2925 5150 882 Size: 617 1299 127 2225 2219 Fragments arranged by size: 2225 2219 1299 617 127 BsaAI yAC'GTr Cuts at: 493 1204 4278 493 Size: 711 3074 2702 Fragments arranged by size: 3074 2702 711 BsaBI GATnn'nnATC Cuts at: 6481 6481 Size: 6487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4294 4676 5311 275 Size: 53 83 186 936 2761 382 635 1451 Fragments arranged by size: 2761 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4920 5318 5592 5739 6150 4920 Size: 398 274 147 411 5257 Fragments arranged by size: 5257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2708 2738 1178 Size: 30 165 30 807 30 468 30 4927 Fragments arranged by size: 4927 807 468 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6487 BseYI C'CCAG_C Cuts at: 2210 2688 2720 5690 6186 2210 Size: 478 32 2970 496 2511 Fragments arranged by size: 2970 2511 496 478 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2292 3032 1163 Size: 573 556 740 4618 Fragments arranged by size: 4618 740 573 556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3937 4698 5726 1540 Size: 275 186 1936 761 1028 2301 Fragments arranged by size: 2301 1936 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2736 2844 3027 4548 4633 5704 2736 Size: 108 183 1521 85 1071 3519 Fragments arranged by size: 3519 1521 1071 183 108 85 BsmI GAATG_Cn' Cuts at: 3998 4091 3998 Size: 93 6394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2672 2736 2844 3027 3131 3351 3567 Size: 399 882 64 108 183 104 220 216 Cuts at: 3567 3806 4548 4633 5704 1391 Size: 239 742 85 1071 2174 Fragments arranged by size: 2174 1071 882 742 399 239 220 216 183 108 104 85 64 BspEI T'CCGG_A Cuts at: 6150 6150 Size: 6487 BspHI T'CATG_A Cuts at: 2154 4376 6106 2154 Size: 2222 1730 2535 Fragments arranged by size: 2535 2222 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3054 3842 844 Size: 2210 788 3489 Fragments arranged by size: 3489 2210 788 BsrBI CCG'CTC Cuts at: 1394 1823 2206 3394 4374 1394 Size: 429 383 1188 980 3507 Fragments arranged by size: 3507 1188 980 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 4982 5156 65 Size: 1183 3734 174 1396 Fragments arranged by size: 3734 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5131 5318 1700 Size: 110 3321 187 2869 Fragments arranged by size: 3321 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6487 BssSI C'ACGA_G Cuts at: 4547 5559 4547 Size: 1012 5475 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6487 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 3670 1184 Size: 2486 4001 BstXI CCAn_nnnn'nTGG Cuts at: 3187 3187 Size: 6487 BstYI r'GATC_y Cuts at: 1972 3707 3817 4184 4569 4586 6038 6124 6136 Size: 1735 110 367 385 17 1452 86 12 Cuts at: 6136 6482 1972 Size: 346 1977 Fragments arranged by size: 1977 1735 1452 385 367 346 110 86 17 12 BstZ17I GTA'TAC Cuts at: 2794 2794 Size: 6487 BtgI C'CryG_G Cuts at: 513 1065 2004 3123 513 Size: 552 939 1119 3877 Fragments arranged by size: 3877 1119 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2875 487 Size: 683 56 1649 4099 Fragments arranged by size: 4099 1649 683 56 BtsI GCAGTG_nn' Cuts at: 2873 3607 3790 4003 4797 4817 2873 Size: 734 183 213 794 20 4543 Fragments arranged by size: 4543 794 734 213 183 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6452 DraI TTT'AAA Cuts at: 3791 3791 Size: 6487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5494 809 Size: 507 4178 1802 Fragments arranged by size: 4178 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3120 3934 4823 Size: 54 1425 50 275 18 1290 814 889 Cuts at: 4823 6452 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1290 889 814 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4417 1353 Size: 3064 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5074 5448 5594 387 Size: 1064 138 3485 374 146 1280 Fragments arranged by size: 3485 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3025 3025 Size: 6487 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2531 2573 2979 3017 3517 3770 Size: 393 605 294 42 406 38 500 253 Cuts at: 3770 4550 5147 5934 6202 1239 Size: 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 605 597 500 406 393 294 268 253 42 38 EcoNI CCTnn'n_nnAGG Cuts at: 2849 3056 2849 Size: 207 6280 EcoO109I rG'GnC_Cy Cuts at: 2281 2668 2980 3115 3157 3583 2281 Size: 387 312 135 42 426 5185 Fragments arranged by size: 5185 426 387 312 135 42 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 EcoRV GAT'ATC Cuts at: 2185 2185 Size: 6487 FspI TGC'GCA Cuts at: 4993 4993 Size: 6487 HaeII r_GCGC'y Cuts at: 1678 2518 3555 5634 1678 Size: 840 1037 2079 2531 Fragments arranged by size: 2531 2079 1037 840 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 2689 2708 2721 Size: 32 135 32 913 32 172 19 13 Cuts at: 2721 2740 2752 2784 3135 3159 3167 3191 5128 Size: 19 12 32 351 24 8 24 1937 Cuts at: 5128 5160 5202 5234 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1937 913 351 172 135 42 32 32 32 32 32 32 24 24 19 19 13 12 8 HincII GTy'rAC Cuts at: 669 1657 1938 3831 4077 669 Size: 988 281 1893 246 3079 Fragments arranged by size: 3079 1893 988 281 246 HindIII A'AGCT_T Cuts at: 748 2467 748 Size: 1719 4768 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2014 2678 5601 5785 1305 Size: 709 664 2923 184 2007 Fragments arranged by size: 2923 2007 709 664 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3122 10 Size: 54 1425 343 1290 3375 Fragments arranged by size: 3375 1425 1290 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 3329 4445 4804 4963 518 Size: 648 954 1209 1116 359 159 2042 Fragments arranged by size: 2042 1209 1116 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2811 3903 4580 5728 5973 1606 Size: 1205 1092 677 1148 245 2120 Fragments arranged by size: 2120 1205 1148 1092 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3123 513 Size: 552 939 1119 3877 Fragments arranged by size: 3877 1119 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NruI TCG'CGA Cuts at: 2534 2534 Size: 6487 NsiI A_TGCA'T Cuts at: 2052 2052 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 3845 5390 1132 Size: 97 2616 1545 2229 Fragments arranged by size: 2616 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 5386 5386 Size: 6487 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6487 PfoI T'CCnGG_A Cuts at: 2619 2700 2844 2619 Size: 81 144 6262 Fragments arranged by size: 6262 144 81 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2980 3115 3157 2281 Size: 699 135 42 5611 Fragments arranged by size: 5611 699 135 42 PshAI GACnn'nnGTC Cuts at: 1613 2331 1613 Size: 718 5769 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PspOMI G'GGCC_C Cuts at: 2668 2668 Size: 6487 PstI C_TGCA'G Cuts at: 830 3839 830 Size: 3009 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 SacI G_AGCT'C Cuts at: 3027 3027 Size: 6487 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 3035 4735 6447 3035 Size: 1700 1712 3075 Fragments arranged by size: 3075 1712 1700 SfcI C'TryA_G Cuts at: 826 1045 2473 3835 4970 5651 5842 826 Size: 219 1428 1362 1135 681 191 1471 Fragments arranged by size: 1471 1428 1362 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 3814 1872 Size: 1942 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4591 5492 5754 820 Size: 197 926 2648 901 262 1553 Fragments arranged by size: 2648 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 2407 4411 5 Size: 47 2355 2004 2081 Fragments arranged by size: 2355 2081 2004 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6487 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 3079 3123 3628 3926 513 Size: 552 939 438 637 44 505 298 3074 Fragments arranged by size: 3074 939 637 552 505 438 298 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3056 4514 4531 4684 4869 5314 Size: 1255 594 882 1458 17 153 185 445 Cuts at: 5314 325 Size: 1498 Fragments arranged by size: 1498 1458 1255 882 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 4733 6445 Size: 274 80 33 51 1443 1056 1700 1712 Cuts at: 6445 96 Size: 138 Fragments arranged by size: 1712 1700 1443 1056 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6447 6479 6447 Size: 32 6455 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6487 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6487 XmaI C'CCGG_G Cuts at: 1870 3812 1870 Size: 1942 4545 XmnI GAAnn'nnTTC Cuts at: 4616 4616 Size: 6487 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI