(Linear) (Six-base) MAPSORT of: KIEE4532.seq Check: 2480 from: 1 to: 1782 KIEE4532.orf With 182 enzymes: SgfI * March 22, 2012 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1049 1782 Size: 1049 733 AccI GT'mk_AC Cuts at: 0 788 1085 1782 Size: 788 297 697 Fragments arranged by size: 788 697 297 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 1012 1512 1765 1782 Size: 568 444 500 253 17 Fragments arranged by size: 568 500 444 253 17 AflIII A'CryG_T Cuts at: 0 306 1782 Size: 306 1476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 133 1782 Size: 133 1649 AlwNI CAG_nnn'CTG Cuts at: 0 690 990 1113 1132 1731 1782 Size: 690 300 123 19 599 51 Fragments arranged by size: 690 599 300 123 51 19 ApaI G_GGCC'C Cuts at: 0 667 1782 Size: 667 1115 AvaI C'yCGr_G Cuts at: 0 362 1782 Size: 362 1420 BamHI G'GATC_C Cuts at: 0 1702 1782 Size: 1702 80 BanI G'GyrC_C Cuts at: 0 1078 1782 Size: 1078 704 BanII G_rGCy'C Cuts at: 0 667 1022 1782 Size: 667 355 760 Fragments arranged by size: 760 667 355 BbsI GAAGACnn'nnnn_ Cuts at: 0 303 967 1782 Size: 303 664 815 Fragments arranged by size: 815 664 303 BbvCI CC'TCA_GC Cuts at: 0 1052 1133 1782 Size: 1052 81 649 Fragments arranged by size: 1052 649 81 BfrBI ATG'CAT Cuts at: 0 45 1782 Size: 45 1737 BglI GCCn_nnn'nGGC Cuts at: 0 601 1782 Size: 601 1181 BlpI GC'TnA_GC Cuts at: 0 605 1021 1782 Size: 605 416 761 Fragments arranged by size: 761 605 416 Bme1580I G_kGCm'C Cuts at: 0 667 1126 1346 1562 1782 Size: 667 459 220 216 220 Fragments arranged by size: 667 459 220 220 216 BmgBI CAC'GTC Cuts at: 0 309 1782 Size: 309 1473 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 217 1782 Size: 217 1565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 526 974 1782 Size: 232 294 448 808 Fragments arranged by size: 808 448 294 232 Bpu10I CC'TnA_GC Cuts at: 0 1052 1133 1782 Size: 1052 81 649 Fragments arranged by size: 1052 649 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 793 920 1782 Size: 793 127 862 Fragments arranged by size: 862 793 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 205 235 703 733 1782 Size: 205 30 468 30 1049 Fragments arranged by size: 1049 468 205 30 30 BseYI C'CCAG_C Cuts at: 0 205 683 715 1782 Size: 205 478 32 1067 Fragments arranged by size: 1067 478 205 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 1027 1782 Size: 287 740 755 Fragments arranged by size: 755 740 287 BsiHKAI G_wGCw'C Cuts at: 0 731 839 1022 1782 Size: 731 108 183 760 Fragments arranged by size: 760 731 183 108 Bsp1286I G_dGCh'C Cuts at: 0 667 731 839 1022 1126 1346 1562 1782 Size: 667 64 108 183 104 220 216 220 Fragments arranged by size: 667 220 220 216 183 108 104 64 BspHI T'CATG_A Cuts at: 0 149 1782 Size: 149 1633 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1049 1782 Size: 1049 733 BsrBI CCG'CTC Cuts at: 0 201 1389 1782 Size: 201 1188 393 Fragments arranged by size: 1188 393 201 BstEII G'GTnAC_C Cuts at: 0 1665 1782 Size: 1665 117 BstXI CCAn_nnnn'nTGG Cuts at: 0 1182 1782 Size: 1182 600 BstYI r'GATC_y Cuts at: 0 1702 1782 Size: 1702 80 BstZ17I GTA'TAC Cuts at: 0 789 1782 Size: 789 993 BtgI C'CryG_G Cuts at: 0 1118 1782 Size: 1118 664 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 870 1782 Size: 870 912 BtsI GCAGTG_nn' Cuts at: 0 868 1602 1782 Size: 868 734 180 Fragments arranged by size: 868 734 180 EaeI y'GGCC_r Cuts at: 0 1115 1782 Size: 1115 667 EcoICRI GAG'CTC Cuts at: 0 1020 1782 Size: 1020 762 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 526 568 974 1012 1512 1765 1782 Size: 232 294 42 406 38 500 253 17 Fragments arranged by size: 500 406 294 253 232 42 38 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 844 1051 1782 Size: 844 207 731 Fragments arranged by size: 844 731 207 EcoO109I rG'GnC_Cy Cuts at: 0 276 663 975 1110 1152 1578 1782 Size: 276 387 312 135 42 426 204 Fragments arranged by size: 426 387 312 276 204 135 42 EcoRV GAT'ATC Cuts at: 0 180 1782 Size: 180 1602 HaeII r_GCGC'y Cuts at: 0 513 1550 1782 Size: 513 1037 232 Fragments arranged by size: 1037 513 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 480 512 684 703 716 735 747 779 Size: 480 32 172 19 13 19 12 32 Cuts at: 779 1130 1154 1162 1186 1782 Size: 351 24 8 24 596 Fragments arranged by size: 596 480 351 172 32 32 24 24 19 19 13 12 8 HindIII A'AGCT_T Cuts at: 0 462 1782 Size: 462 1320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 9 673 1782 Size: 9 664 1109 Fragments arranged by size: 1109 664 9 MscI TGG'CCA Cuts at: 0 1117 1782 Size: 1117 665 MslI CAynn'nnrTG Cuts at: 0 115 1324 1782 Size: 115 1209 458 Fragments arranged by size: 1209 458 115 MspA1I CmG'CkG Cuts at: 0 806 1782 Size: 806 976 NcoI C'CATG_G Cuts at: 0 1118 1782 Size: 1118 664 NruI TCG'CGA Cuts at: 0 529 1782 Size: 529 1253 NsiI A_TGCA'T Cuts at: 0 47 1782 Size: 47 1735 PfoI T'CCnGG_A Cuts at: 0 614 695 839 1782 Size: 614 81 144 943 Fragments arranged by size: 943 614 144 81 PpuMI rG'GwC_Cy Cuts at: 0 276 975 1110 1152 1782 Size: 276 699 135 42 630 Fragments arranged by size: 699 630 276 135 42 PshAI GACnn'nnGTC Cuts at: 0 326 1782 Size: 326 1456 PspOMI G'GGCC_C Cuts at: 0 663 1782 Size: 663 1119 SacI G_AGCT'C Cuts at: 0 1022 1782 Size: 1022 760 ScaI AGT'ACT Cuts at: 0 1030 1782 Size: 1030 752 SfcI C'TryA_G Cuts at: 0 468 1782 Size: 468 1314 SspI AAT'ATT Cuts at: 0 402 1782 Size: 402 1380 StyI C'CwwG_G Cuts at: 0 437 1074 1118 1623 1782 Size: 437 637 44 505 159 Fragments arranged by size: 637 505 437 159 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 169 1051 1782 Size: 169 882 731 Fragments arranged by size: 882 731 169 TatI w'GTAC_w Cuts at: 0 1028 1782 Size: 1028 754 Enzymes that do cut: AarI AccI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI BanI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NruI NsiI PfoI PpuMI PshAI PspOMI SacI ScaI SfcI SspI StyI TaqII TatI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BglII BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoRI FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI