Restriction map for
pFN21AE0322
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BseAI BsgI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII Eam1105I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfuI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 83 GGTACC G^GTACC
AccI 1029 GTCTAC GT^[AC][GT]AC
AcyI 286 GGCGCC G[GA]^CG[CT]C
AlwNI 1369 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 619 AGCGCT AGC^GCT
ApaI 381 GGGCCC GGGCC^C
Asp718I 83 GGTACC G^GTACC
BbeI 286 GGCGCC GGCGC^C
BcgI 404 CGACCACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1529 CTAG C^TAG
BlpI 1395 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1395 GCTAAGC GC^T[ACGT]AGC
BsaHI 286 GGCGCC G[GA]^CG[CT]C
BsaWI 666 TCCGGT [AT]^CCGG[AT]
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmI 921 GAATGC GAATGC(1/-1)
BspHI 1583 TCATGA T^CATGA
BsrBI 1539 CCGCTC CCGCTC(-3/-3)
BssSI 1210 CTCGTG CACGAG(-5/-1)
BstF5I 269 GGATG GGATG(2/0)
CelII 1395 GCTAAGC GC^T[ACGT]AGC
DrdI 1288 GACTCCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 35 CGGCCG C^GGCCG
EarI 1387 CTCTTC CTCTTC(1/4)
EclXI 35 CGGCCG C^GGCCG
Eco47III 619 AGCGCT AGC^GCT
Eco52I 35 CGGCCG C^GGCCG
EcoRV 763 GATATC GAT^ATC
FokI 269 GGATG GGATG(9/13)
Hin1I 286 GGCGCC G[GA]^CG[CT]C
KasI 286 GGCGCC G^GCGCC
KpnI 83 GGTACC GGTAC^C
Ksp632I 1387 CTCTTC CTCTTC(1/4)
MaeI 1529 CTAG C^TAG
MseI 1178 TTAA T^TAA
NarI 286 GGCGCC GG^CGCC
NcoI 866 CCATGG C^CATGG
NheI 1528 GCTAGC G^CTAGC
NspI 1227 GCATGC [GA]CATG^[CT]
RcaI 1583 TCATGA T^CATGA
SapI 1386 GCTCTTC GCTCTTC(1/4)
SfiI 237 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 286 GGCGCC GGC^GCC
SgrAI 288 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 718 CTCAAG C^T[CT][GA]AG
SphI 1227 GCATGC GCATG^C
Tru9I 1178 TTAA T^TAA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 392 CCTCAGG CC^T[ACGT]AGG
496 CCTAAGG
AspHI 639 GAGCAC G[AT]GC[AT]^C
715 GTGCTC
AspI 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1288 GACTCCGTC
BamHI 72 GGATCC G^GATCC
324 GGATCC
BsiHKAI 639 GAGCAC G[AT]GC[AT]^C
715 GTGCTC
BspMI 566 ACCTGC ACCTGC(4/8)
1658 ACCTGC
BssHII 54 GCGCGC G^CGCGC
1343 GCGCGC
BstYI 72 GGATCC [GA]^GATC[CT]
324 GGATCC
Bsu36I 392 CCTCAGG CC^T[ACGT]AGG
496 CCTAAGG
DsaI 372 CCACGG C^C[GA][CT]GG
866 CCATGG
Eco81I 392 CCTCAGG CC^T[ACGT]AGG
496 CCTAAGG
MboII 452 TCTTC GAAGA(8/7)
1388 TCTTC
MflI 72 GGATCC [GA]^GATC[CT]
324 GGATCC
NaeI 289 GCCGGC GCC^GGC
680 GCCGGC
NgoMIV 289 GCCGGC G^CCGGC
680 GCCGGC
PflFI 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1288 GACTCCGTC
PpuMI 892 GGGACCT [GA]G^G[AT]CC[CT]
1715 AGGTCCT
PvuII 952 CAGCTG CAG^CTG
1246 CAGCTG
TaqI 22 TCGA T^CGA
1391 TCGA
TfiI 301 GATTC G^A[AT]TC
770 GAATC
Tth111I 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1288 GACTCCGTC
TthHB8I 22 TCGA T^CGA
1391 TCGA
XhoII 72 GGATCC [GA]^GATC[CT]
324 GGATCC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TseI Tsp45I TspRI XcmI
XmaCI XmaI