(Circular) (Six-base) MAPSORT of: pFN21AE0322.seq Check: 2056 from: 1 to: 6445 pFN21AE0322 6445 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 3788 1937 Size: 1098 753 4594 Fragments arranged by size: 4594 1098 753 Acc65I G'GTAC_C Cuts at: 2088 3766 2088 Size: 1678 4767 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3343 3440 4508 5892 6160 1632 Size: 1711 97 1068 1384 268 1917 Fragments arranged by size: 1917 1711 1384 1068 268 97 AfeI AGC'GCT Cuts at: 2626 2626 Size: 6445 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 2931 Size: 32 6413 AlwNI CAG_nnn'CTG Cuts at: 3379 4188 5760 3379 Size: 809 1572 4064 Fragments arranged by size: 4064 1572 809 ApaI G_GGCC'C Cuts at: 2390 2390 Size: 6445 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 3754 3980 4132 6241 1493 Size: 2261 226 152 2109 1697 Fragments arranged by size: 2261 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2194 2383 2894 3458 3770 1870 Size: 73 251 189 511 564 312 4545 Fragments arranged by size: 4545 564 511 312 251 189 73 BamHI G'GATC_C Cuts at: 2077 2329 3775 4142 2077 Size: 252 1446 367 4380 Fragments arranged by size: 4380 1446 367 252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2180 2291 2545 3766 Size: 325 217 627 301 92 111 254 1221 Cuts at: 3766 5221 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1221 627 325 301 254 217 111 92 BanII G_rGCy'C Cuts at: 1391 2207 2316 2390 2879 3393 3764 1391 Size: 816 109 74 489 514 371 4072 Fragments arranged by size: 4072 816 514 489 371 109 74 BbeI G_GCGC'C Cuts at: 2295 2295 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2398 2432 4636 4670 1903 Size: 34 461 34 2204 34 3678 Fragments arranged by size: 3678 2204 461 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2249 3281 3374 Size: 107 122 71 631 605 577 1032 93 Cuts at: 3374 5056 136 Size: 1682 1525 Fragments arranged by size: 1682 1525 1032 631 605 577 122 107 93 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 3401 3862 3401 Size: 461 5984 Bme1580I G_kGCm'C Cuts at: 1790 2390 4506 5662 1790 Size: 600 2116 1156 2573 Fragments arranged by size: 2573 2116 1156 600 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2008 3081 3544 Size: 416 53 83 399 24 591 1073 463 Cuts at: 3544 4176 5134 442 Size: 632 958 1753 Fragments arranged by size: 1753 1073 958 632 591 463 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3537 1056 Size: 2481 3964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2773 2773 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2191 2954 2967 5105 1239 Size: 952 763 13 2138 2579 Fragments arranged by size: 2579 2138 952 763 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4570 5435 5733 2708 Size: 1862 865 298 3420 Fragments arranged by size: 3420 1862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5108 882 Size: 617 3609 2219 Fragments arranged by size: 3609 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4236 493 Size: 711 3032 2702 Fragments arranged by size: 3032 2702 711 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 4252 4634 5269 Size: 53 83 186 936 759 1960 382 635 Cuts at: 5269 275 Size: 1451 Fragments arranged by size: 1960 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2671 4878 5276 5550 5697 6108 2671 Size: 2207 398 274 147 411 3008 Fragments arranged by size: 3008 2207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2578 2608 1178 Size: 30 165 30 597 30 548 30 5015 Fragments arranged by size: 5015 597 548 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2226 2731 3275 3278 1186 Size: 1040 505 544 3 4353 Fragments arranged by size: 4353 1040 544 505 3 BseYI C'CCAG_C Cuts at: 3491 5648 6144 3491 Size: 2157 496 3792 Fragments arranged by size: 3792 2157 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5872 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3895 4656 5684 1540 Size: 275 186 42 1852 761 1028 2301 Fragments arranged by size: 2301 1852 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2648 2724 4506 4591 5662 2648 Size: 76 1782 85 1071 3431 Fragments arranged by size: 3431 1782 1071 85 76 BsmI GAATG_Cn' Cuts at: 2932 3956 4049 2932 Size: 1024 93 5328 Fragments arranged by size: 5328 1024 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2316 2390 2648 2724 2879 3393 Size: 399 417 109 74 258 76 155 514 Cuts at: 3393 3764 4506 4591 5662 1391 Size: 371 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 417 399 371 258 155 109 85 76 74 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 3588 4334 6064 3588 Size: 746 1730 3969 Fragments arranged by size: 3969 1730 746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2580 3672 3800 844 Size: 1736 1092 128 3489 Fragments arranged by size: 3489 1736 1092 128 BsrBI CCG'CTC Cuts at: 1394 1823 3546 4332 1394 Size: 429 1723 786 3507 Fragments arranged by size: 3507 1723 786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2294 2685 5089 5276 1700 Size: 110 275 209 391 2404 187 2869 Fragments arranged by size: 2869 2404 391 275 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 2059 3348 1925 Size: 134 1289 5022 Fragments arranged by size: 5022 1289 134 BssSI C'ACGA_G Cuts at: 3215 4505 5517 3215 Size: 1290 1012 4143 Fragments arranged by size: 4143 1290 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6445 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstYI r'GATC_y Cuts at: 1972 2077 2329 3775 4142 4527 4544 5996 6082 Size: 105 252 1446 367 385 17 1452 86 Cuts at: 6082 6094 6440 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 252 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2398 2502 2398 Size: 104 6341 BtgI C'CryG_G Cuts at: 513 1065 2004 2377 2871 513 Size: 552 939 373 494 4087 Fragments arranged by size: 4087 939 552 494 373 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5706 Fragments arranged by size: 5706 683 56 BtsI GCAGTG_nn' Cuts at: 3607 3961 4755 4775 3607 Size: 354 794 20 5277 Fragments arranged by size: 5277 794 354 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3299 5452 809 Size: 507 1983 2153 1802 Fragments arranged by size: 2153 1983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2241 3649 Size: 54 1425 50 275 18 210 201 1408 Cuts at: 3649 3892 4781 6410 8 Size: 243 889 1629 43 Fragments arranged by size: 1629 1425 1408 889 275 243 210 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 3892 1537 Size: 275 228 1852 4090 Fragments arranged by size: 4090 1852 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 3398 4375 1353 Size: 2045 977 3423 Fragments arranged by size: 3423 2045 977 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5032 5406 5552 387 Size: 1064 138 3443 374 146 1280 Fragments arranged by size: 3443 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2954 2967 3343 3440 4508 5105 Size: 393 559 763 13 376 97 1068 597 Cuts at: 5105 5892 6160 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1068 787 763 597 559 393 376 268 97 13 EcoO109I rG'GnC_Cy Cuts at: 2387 2898 2998 3721 2387 Size: 511 100 723 5111 Fragments arranged by size: 5111 723 511 100 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 EcoRV GAT'ATC Cuts at: 2770 2770 Size: 6445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3067 3218 3067 Size: 151 6294 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 2099 2265 2295 2628 3271 5592 1678 Size: 421 166 30 333 643 2321 2531 Fragments arranged by size: 2531 2321 643 421 333 166 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 5086 5118 5160 Size: 32 135 32 1006 32 2476 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 2476 1006 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 2291 2291 Size: 6445 KpnI G_GTAC'C Cuts at: 2092 3770 2092 Size: 1678 4767 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5559 5743 1305 Size: 4254 184 2007 Fragments arranged by size: 4254 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4623 Fragments arranged by size: 4623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4403 4762 4921 518 Size: 648 3237 359 159 2042 Fragments arranged by size: 3237 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2923 2959 3253 3861 4538 5686 5931 Size: 444 873 36 294 608 677 1148 245 Cuts at: 5931 1606 Size: 2120 Fragments arranged by size: 2120 1148 873 677 608 444 294 245 36 NaeI GCC'GGC Cuts at: 2296 2687 2296 Size: 391 6054 NarI GG'CG_CC Cuts at: 2292 2292 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 2004 2871 513 Size: 552 939 867 4087 Fragments arranged by size: 4087 939 867 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NgoMIV G'CCGG_C Cuts at: 2294 2685 2294 Size: 391 6054 NheI G'CTAG_C Cuts at: 1052 3533 1052 Size: 2481 3964 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 3236 3803 5348 1132 Size: 97 2007 567 1545 2229 Fragments arranged by size: 2229 2007 1545 567 97 PasI CC'CwG_GG Cuts at: 1348 2318 2492 1348 Size: 970 174 5301 Fragments arranged by size: 5301 970 174 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6445 PfoI T'CCnGG_A Cuts at: 3335 3507 3335 Size: 172 6273 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2726 2758 4514 4546 6027 6059 2726 Size: 32 1756 32 1481 32 3112 Fragments arranged by size: 3112 1756 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2898 3721 2898 Size: 823 5622 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 2386 2386 Size: 6445 PstI C_TGCA'G Cuts at: 830 2363 2586 3429 3797 830 Size: 1533 223 843 368 3478 Fragments arranged by size: 3478 1533 843 368 223 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2959 3253 2959 Size: 294 6151 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SapI GCTCTTCn'nnn_ Cuts at: 3398 3398 Size: 6445 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2359 2582 3425 3793 4928 5609 5800 Size: 219 1314 223 843 368 1135 681 191 Cuts at: 5800 826 Size: 1471 Fragments arranged by size: 1471 1314 1135 843 681 368 223 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2249 2249 Size: 6445 SfoI GGC'GCC Cuts at: 2293 2293 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 2294 1700 Size: 594 5851 SmaI CCC'GGG Cuts at: 1872 2196 2385 2896 3772 1872 Size: 324 189 511 876 4545 Fragments arranged by size: 4545 876 511 324 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 4549 5450 5712 820 Size: 197 926 780 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 780 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3236 3803 1132 Size: 2104 567 3774 Fragments arranged by size: 3774 2104 567 SrfI GCCC'GGGC Cuts at: 2196 2385 2196 Size: 189 6256 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6445 StyI C'CwwG_G Cuts at: 513 1065 2004 2871 2991 3325 3884 513 Size: 552 939 867 120 334 559 3074 Fragments arranged by size: 3074 939 867 559 552 334 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2390 3086 4472 4489 4642 4827 Size: 1255 665 145 696 1386 17 153 185 Cuts at: 4827 5272 325 Size: 445 1498 Fragments arranged by size: 1498 1386 1255 696 665 445 185 153 145 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2118 4691 Size: 274 80 33 51 1443 54 87 2573 Cuts at: 4691 6403 96 Size: 1712 138 Fragments arranged by size: 2573 1712 1443 274 138 87 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 3092 3124 6405 6437 2000 Size: 32 1060 32 3281 32 2008 Fragments arranged by size: 3281 2008 1060 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2843 3296 1316 Size: 1527 453 4465 Fragments arranged by size: 4465 1527 453 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 2998 3094 2419 Size: 579 96 5770 Fragments arranged by size: 5770 579 96 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 2194 2383 2894 3770 1870 Size: 324 189 511 876 4545 Fragments arranged by size: 4545 876 511 324 189 XmnI GAAnn'nnTTC Cuts at: 4574 4574 Size: 6445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SwaI