Restriction map for
pFN21AE0322
(Linear) (Six-base) MAPSORT of: KIEE0322.seq Check: 6542 from: 1 to: 1740
KIEE0322.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1030 1740
Size: 1030 710
Acc65I G'GTAC_C
Cuts at: 0 83 1740
Size: 83 1657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1338 1435 1740
Size: 1338 97 305
Fragments arranged by size:
1338 305 97
AfeI AGC'GCT
Cuts at: 0 621 1740
Size: 621 1119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 1740
Size: 926 32 782
Fragments arranged by size:
926 782 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1374 1740
Size: 1374 366
ApaI G_GGCC'C
Cuts at: 0 385 1740
Size: 385 1355
AvaI C'yCGr_G
Cuts at: 0 189 378 889 1453 1740
Size: 189 189 511 564 287
Fragments arranged by size:
564 511 287 189 189
BamHI G'GATC_C
Cuts at: 0 72 324 1740
Size: 72 252 1416
Fragments arranged by size:
1416 252 72
BanI G'GyrC_C
Cuts at: 0 83 175 286 540 1740
Size: 83 92 111 254 1200
Fragments arranged by size:
1200 254 111 92 83
BanII G_rGCy'C
Cuts at: 0 202 311 385 874 1388 1740
Size: 202 109 74 489 514 352
Fragments arranged by size:
514 489 352 202 109 74
BbeI G_GCGC'C
Cuts at: 0 290 1740
Size: 290 1450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 393 427 1740
Size: 393 34 1313
Fragments arranged by size:
1313 393 34
BglI GCCn_nnn'nGGC
Cuts at: 0 244 1276 1369 1740
Size: 244 1032 93 371
Fragments arranged by size:
1032 371 244 93
BlpI GC'TnA_GC
Cuts at: 0 1396 1740
Size: 1396 344
Bme1580I G_kGCm'C
Cuts at: 0 385 1740
Size: 385 1355
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 1076 1539 1740
Size: 3 1073 463 201
Fragments arranged by size:
1073 463 201 3
BmtI G_CTAG'C
Cuts at: 0 1532 1740
Size: 1532 208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 768 1740
Size: 768 972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 949 962 1740
Size: 186 763 13 778
Fragments arranged by size:
778 763 186 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1740
Size: 703 1037
BsaHI Gr'CG_yC
Cuts at: 0 287 1740
Size: 287 1453
BsaWI w'CCGG_w
Cuts at: 0 666 1740
Size: 666 1074
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 573 603 1740
Size: 25 548 30 1137
Fragments arranged by size:
1137 548 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 726 1270 1273 1740
Size: 221 505 544 3 467
Fragments arranged by size:
544 505 467 221 3
BseYI C'CCAG_C
Cuts at: 0 1486 1740
Size: 1486 254
BsiEI CG_ry'CG
Cuts at: 0 38 1740
Size: 38 1702
BsiHKAI G_wGCw'C
Cuts at: 0 643 719 1740
Size: 643 76 1021
Fragments arranged by size:
1021 643 76
BsmI GAATG_Cn'
Cuts at: 0 927 1740
Size: 927 813
Bsp1286I G_dGCh'C
Cuts at: 0 202 311 385 643 719 874 1388 1740
Size: 202 109 74 258 76 155 514 352
Fragments arranged by size:
514 352 258 202 155 109 76 74
BspHI T'CATG_A
Cuts at: 0 1583 1740
Size: 1583 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 575 1667 1740
Size: 575 1092 73
Fragments arranged by size:
1092 575 73
BsrBI CCG'CTC
Cuts at: 0 1541 1740
Size: 1541 199
BsrFI r'CCGG_y
Cuts at: 0 80 289 680 1740
Size: 80 209 391 1060
Fragments arranged by size:
1060 391 209 80
BssHII G'CGCG_C
Cuts at: 0 54 1343 1740
Size: 54 1289 397
Fragments arranged by size:
1289 397 54
BssSI C'ACGA_G
Cuts at: 0 1210 1740
Size: 1210 530
BstYI r'GATC_y
Cuts at: 0 72 324 1740
Size: 72 252 1416
Fragments arranged by size:
1416 252 72
Bsu36I CC'TnA_GG
Cuts at: 0 393 497 1740
Size: 393 104 1243
Fragments arranged by size:
1243 393 104
BtgI C'CryG_G
Cuts at: 0 372 866 1740
Size: 372 494 874
Fragments arranged by size:
874 494 372
BtsI GCAGTG_nn'
Cuts at: 0 1602 1740
Size: 1602 138
DrdI GACnn_nn'nnGTC
Cuts at: 0 1294 1740
Size: 1294 446
EaeI y'GGCC_r
Cuts at: 0 35 236 1644 1740
Size: 35 201 1408 96
Fragments arranged by size:
1408 201 96 35
EagI C'GGCC_G
Cuts at: 0 35 1740
Size: 35 1705
EarI CTCTTCn'nnn_
Cuts at: 0 1393 1740
Size: 1393 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 949 962 1338 1435 1740
Size: 186 763 13 376 97 305
Fragments arranged by size:
763 376 305 186 97 13
EcoO109I rG'GnC_Cy
Cuts at: 0 382 893 993 1716 1740
Size: 382 511 100 723 24
Fragments arranged by size:
723 511 382 100 24
EcoRV GAT'ATC
Cuts at: 0 765 1740
Size: 765 975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1062 1213 1740
Size: 1062 151 527
Fragments arranged by size:
1062 527 151
HaeII r_GCGC'y
Cuts at: 0 94 260 290 623 1266 1740
Size: 94 166 30 333 643 474
Fragments arranged by size:
643 474 333 166 94 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 1740
Size: 573 32 1135
Fragments arranged by size:
1135 573 32
KasI G'GCGC_C
Cuts at: 0 286 1740
Size: 286 1454
KpnI G_GTAC'C
Cuts at: 0 87 1740
Size: 87 1653
MspA1I CmG'CkG
Cuts at: 0 45 918 954 1248 1740
Size: 45 873 36 294 492
Fragments arranged by size:
873 492 294 45 36
NaeI GCC'GGC
Cuts at: 0 291 682 1740
Size: 291 391 1058
Fragments arranged by size:
1058 391 291
NarI GG'CG_CC
Cuts at: 0 287 1740
Size: 287 1453
NcoI C'CATG_G
Cuts at: 0 866 1740
Size: 866 874
NgoMIV G'CCGG_C
Cuts at: 0 289 680 1740
Size: 289 391 1060
Fragments arranged by size:
1060 391 289
NheI G'CTAG_C
Cuts at: 0 1528 1740
Size: 1528 212
NspI r_CATG'y
Cuts at: 0 1231 1740
Size: 1231 509
PasI CC'CwG_GG
Cuts at: 0 313 487 1740
Size: 313 174 1253
Fragments arranged by size:
1253 313 174
PfoI T'CCnGG_A
Cuts at: 0 1330 1502 1740
Size: 1330 172 238
Fragments arranged by size:
1330 238 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1740
Size: 721 32 987
Fragments arranged by size:
987 721 32
PpuMI rG'GwC_Cy
Cuts at: 0 893 1716 1740
Size: 893 823 24
Fragments arranged by size:
893 823 24
PspOMI G'GGCC_C
Cuts at: 0 381 1740
Size: 381 1359
PstI C_TGCA'G
Cuts at: 0 358 581 1424 1740
Size: 358 223 843 316
Fragments arranged by size:
843 358 316 223
PvuII CAG'CTG
Cuts at: 0 954 1248 1740
Size: 954 294 492
Fragments arranged by size:
954 492 294
SapI GCTCTTCn'nnn_
Cuts at: 0 1393 1740
Size: 1393 347
SfcI C'TryA_G
Cuts at: 0 354 577 1420 1740
Size: 354 223 843 320
Fragments arranged by size:
843 354 320 223
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 244 1740
Size: 244 1496
SfoI GGC'GCC
Cuts at: 0 288 1740
Size: 288 1452
SgrAI Cr'CCGG_yG
Cuts at: 0 289 1740
Size: 289 1451
SmaI CCC'GGG
Cuts at: 0 191 380 891 1740
Size: 191 189 511 849
Fragments arranged by size:
849 511 191 189
SmlI C'TyrA_G
Cuts at: 0 718 1740
Size: 718 1022
SphI G_CATG'C
Cuts at: 0 1231 1740
Size: 1231 509
SrfI GCCC'GGGC
Cuts at: 0 191 380 1740
Size: 191 189 1360
Fragments arranged by size:
1360 191 189
StyI C'CwwG_G
Cuts at: 0 866 986 1320 1740
Size: 866 120 334 420
Fragments arranged by size:
866 420 334 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 385 1081 1740
Size: 240 145 696 659
Fragments arranged by size:
696 659 240 145
TatI w'GTAC_w
Cuts at: 0 26 113 1740
Size: 26 87 1627
Fragments arranged by size:
1627 87 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1087 1119 1740
Size: 27 1060 32 621
Fragments arranged by size:
1060 621 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 838 1291 1740
Size: 838 453 449
Fragments arranged by size:
838 453 449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 993 1089 1740
Size: 414 579 96 651
Fragments arranged by size:
651 579 414 96
XmaI C'CCGG_G
Cuts at: 0 189 378 889 1740
Size: 189 189 511 851
Fragments arranged by size:
851 511 189 189
Enzymes that do cut:
AccI Acc65I AcuI AfeI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BcgI BglI BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BssSI BstYI Bsu36I BtgI BtsI
DrdI EaeI EagI EarI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I KasI KpnI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PfoI PpiI PpuMI PspOMI
PstI PvuII SapI SfcI SfiI SfoI SgrAI SmaI
SmlI SphI SrfI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BciVI BclI BfrBI BglII BmgBI Bpu10I
BsaI BsaAI BsaBI BsgI BsiWI BsmBI BspEI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI MslI NdeI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SgfI SnaBI SpeI SspI StuI SwaI XbaI XhoI
XmnI ZraI