################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI KpnI MaeI MamI MluI MluNI MroI MscI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 520 AAATTT [GA]^AATT[CT] AfaI 702 GTAC GT^AC AlwNI 111 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 520 AAATTT [GA]^AATT[CT] AspHI 145 GAGCAC G[AT]GC[AT]^C AvaI 169 CCCGAG C^[CT]CG[GA]G BamHI 591 GGATCC G^GATCC BanI 154 GGCGCC G^G[CT][GA]CC BanII 667 GAGCCC G[GA]GC[CT]^C BbeI 154 GGCGCC GGCGC^C BbsI 127 GAAGAC GAAGAC(2/6) BglI 133 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 127 GAAGAC GAAGAC(2/6) BsgI 230 GTGCAG GTGCAG(16/14) BsiEI 453 CGATCG CG[GA][CT]^CG BsiHKAI 145 GAGCAC G[AT]GC[AT]^C BsmI 497 GCATTC GAATGC(1/-1) BsoBI 169 CCCGAG C^[CT]CG[GA]G BsrFI 372 GCCGGC [GA]^CCGG[CT] BsrI 221 ACTGG ACTGG(1/-1) BstYI 591 GGATCC [GA]^GATC[CT] Cfr10I 372 GCCGGC [GA]^CCGG[CT] DraII 192 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 150 CCGCGG C^C[GA][CT]GG EarI 314 GAAGAG CTCTTC(1/4) EcoO109I 192 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 719 CCAAGG C^C[AT][AT]GG HapII 373 CCGG C^CGG HindIII 767 AAGCTT A^AGCTT HpaI 262 GTTAAC GTT^AAC HpaII 373 CCGG C^CGG KasI 154 GGCGCC G^GCGCC Ksp632I 314 GAAGAG CTCTTC(1/4) KspI 150 CCGCGG CCGC^GG MaeII 540 ACGT A^CGT MaeIII 50 GTGAC ^GT[ACGT]AC MfeI 443 CAATTG C^AATTG MflI 591 GGATCC [GA]^GATC[CT] MslI 717 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 373 CCGG C^CGG MunI 443 CAATTG C^AATTG NaeI 372 GCCGGC GCC^GGC NarI 154 GGCGCC GG^CGCC NgoMIV 372 GCCGGC G^CCGGC PleI 355 GAGTC GAGTC(4/5) PstI 650 CTGCAG CTGCA^G PvuI 453 CGATCG CGAT^CG RsaI 702 GTAC GT^AC SacII 150 CCGCGG CCGC^GG SalI 536 GTCGAC G^TCGAC SfoI 154 GGCGCC GGC^GCC SmlI 786 CTTGAG C^T[CT][GA]AG StyI 719 CCAAGG C^C[AT][AT]GG Tsp45I 50 GTGAC ^GT[GC]AC XhoII 591 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 592 GATCC GGATC(4/5) 591 GGATC AvaII 254 GGACC G^G[AT]CC 549 GGACC BbvI 659 GCAGC GCAGC(8/12) 41 GCTGC BmyI 145 GAGCAC G[AGT]GC[ACT]^C 667 GAGCCC BpmI 596 CTCCAG CTGGAG(16/14) 117 CTGGAG BsiYI 71 CCGCGCGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 104 CCGACGGCAGG BslI 71 CCGCGCGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 104 CCGACGGCAGG Bsp1286I 145 GAGCAC G[AGT]GC[ACT]^C 667 GAGCCC BssKI 84 CCTGG ^CC[ACGT]GG 493 CCTGG BstNI 84 CCTGG CC^[AT]GG 493 CCTGG DdeI 683 CTCAG C^T[ACGT]AG 798 CTCAG EcoRII 84 CCTGG ^CC[AT]GG 493 CCTGG HaeII 113 GGCGCT [GA]GCGC^[CT] 154 GGCGCC HgaI 52 GACGC GACGC(5/10) 130 GACGC HincII 262 GTTAAC GT[CT]^[GA]AC 536 GTCGAC HindII 262 GTTAAC GT[CT]^[GA]AC 536 GTCGAC HphI 49 GGTGA GGTGA(8/7) 723 GGTGA MvaI 84 CCTGG CC^[AT]GG 493 CCTGG ScrFI 84 CCTGG CC^[ACGT]GG 493 CCTGG SfaNI 212 GATGC GCATC(5/9) 30 GCATC SfcI 635 CTGTAG C^T[GA][CT]AG 650 CTGCAG TaqI 486 TCGA T^CGA 537 TCGA TfiI 346 GATTC G^A[AT]TC 646 GAATC TseI 41 GCTGC G^C[AT]GC 659 GCAGC Tsp509I 444 AATT ^AATT 521 AATT TthHB8I 486 TCGA T^CGA 537 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccI AccII AciI AcyI AluI BsaHI BsaJI BseRI BsmAI BsmFI BstF5I BstUI Cac8I CfoI Cfr13I DpnII FauI Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HinfI ItaI MboI MboII MnlI MseI MspA1I MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I Tru9I