(Circular) (Six-base) MAPSORT of: pFN21AE5307.seq Check: 1586 from: 1 to: 5515 pFN21AE5307 5515 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2369 2542 2643 2858 1937 Size: 432 173 101 215 4594 Fragments arranged by size: 4594 432 215 173 101 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 3578 4962 5230 1632 Size: 582 1364 1384 268 1917 Fragments arranged by size: 1917 1384 1364 582 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 2726 1166 Size: 1560 3955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 2121 3258 4830 2121 Size: 1137 1572 2806 Fragments arranged by size: 2806 1572 1137 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2525 2824 3050 3202 5311 1493 Size: 1032 299 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2840 1870 Size: 73 231 666 4545 Fragments arranged by size: 4545 666 231 73 BamHI G'GATC_C Cuts at: 2596 2845 3212 2596 Size: 249 367 4899 Fragments arranged by size: 4899 367 249 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2836 4291 618 Size: 325 217 627 372 677 1455 1842 Fragments arranged by size: 1842 1455 677 627 372 325 217 BanII G_rGCy'C Cuts at: 1391 2676 2834 1391 Size: 1285 158 4072 Fragments arranged by size: 4072 1285 158 BbeI G_GCGC'C Cuts at: 2163 2163 Size: 5515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 928 Size: 971 240 4304 Fragments arranged by size: 4304 971 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 4126 136 Size: 107 122 71 631 605 472 1982 1525 Fragments arranged by size: 1982 1525 631 605 472 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 3576 4732 1790 Size: 1786 1156 2573 Fragments arranged by size: 2573 1786 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 3246 4204 Size: 416 53 83 399 24 819 1010 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 1010 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2586 4175 1239 Size: 904 443 1589 2579 Fragments arranged by size: 2579 1589 904 443 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2812 3640 4505 4803 2812 Size: 828 865 298 3524 Fragments arranged by size: 3524 865 828 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2136 2160 3322 Size: 53 83 186 936 525 78 24 1162 Cuts at: 3322 3704 4339 275 Size: 382 635 1451 Fragments arranged by size: 1451 1162 936 635 525 382 186 83 78 53 24 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 2744 2774 1178 Size: 30 165 30 948 30 363 30 3919 Fragments arranged by size: 3919 948 363 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2291 2428 1186 Size: 970 135 137 4273 Fragments arranged by size: 4273 970 137 135 BseYI C'CCAG_C Cuts at: 4718 5214 4718 Size: 496 5019 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2256 1163 Size: 573 520 4422 Fragments arranged by size: 4422 573 520 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2461 2965 3726 4754 1540 Size: 275 186 460 504 761 1028 2301 Fragments arranged by size: 2301 1028 761 504 460 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 3576 3661 4732 2154 Size: 1422 85 1071 2937 Fragments arranged by size: 2937 1422 1071 85 BsmI GAATG_Cn' Cuts at: 2502 3026 3119 2502 Size: 524 93 4898 Fragments arranged by size: 4898 524 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2676 2834 3576 3661 4732 1391 Size: 399 364 522 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 399 364 158 85 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2377 4159 4346 1700 Size: 110 567 1782 187 2869 Fragments arranged by size: 2869 1782 567 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2596 2845 3212 3597 3614 5066 5152 5164 Size: 624 249 367 385 17 1452 86 12 Cuts at: 5164 5510 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 624 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2155 513 Size: 552 939 151 3873 Fragments arranged by size: 3873 939 552 151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2095 487 Size: 683 56 869 3907 Fragments arranged by size: 3907 869 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480 Size: 54 1425 50 275 18 1132 889 1629 Cuts at: 5480 8 Size: 43 Fragments arranged by size: 1629 1425 1132 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2314 3445 1353 Size: 961 1131 3423 Fragments arranged by size: 3423 1131 961 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2214 2586 3578 4175 4962 5230 Size: 393 511 71 372 992 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 992 787 597 511 393 372 268 71 EcoO109I rG'GnC_Cy Cuts at: 2198 2198 Size: 5515 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2122 2163 4662 1678 Size: 444 41 2499 2531 Fragments arranged by size: 2531 2499 444 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2351 2383 2636 Size: 32 135 32 743 32 4 32 253 Cuts at: 2636 2668 2671 2693 2703 2725 4156 4188 4230 Size: 32 3 22 10 22 1431 32 42 Cuts at: 4230 4262 1373 Size: 32 2626 Fragments arranged by size: 2626 1431 743 253 135 42 32 32 32 32 32 32 32 22 22 10 4 3 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2543 2859 3105 669 Size: 988 281 331 274 316 246 3079 Fragments arranged by size: 3079 988 331 316 281 274 246 HindIII A'AGCT_T Cuts at: 748 2772 748 Size: 2024 3491 HpaI GTT'AAC Cuts at: 2269 3105 2269 Size: 836 4679 KasI G'GCGC_C Cuts at: 2159 2159 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 2448 3114 2448 Size: 666 4849 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3693 Fragments arranged by size: 3693 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2726 3473 3832 3991 518 Size: 648 1560 747 359 159 2042 Fragments arranged by size: 2042 1560 747 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2157 2606 2931 3608 4756 5001 1606 Size: 440 111 449 325 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 449 440 325 245 111 NaeI GCC'GGC Cuts at: 2379 2379 Size: 5515 NarI GG'CG_CC Cuts at: 2160 2160 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NgoMIV G'CCGG_C Cuts at: 2377 2377 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PstI C_TGCA'G Cuts at: 830 2659 2867 830 Size: 1829 208 3478 Fragments arranged by size: 3478 1829 208 PvuI CG_AT'CG Cuts at: 2001 2461 2001 Size: 460 5055 SacII CC_GC'GG Cuts at: 2158 2158 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2541 2857 1936 Size: 605 316 4594 Fragments arranged by size: 4594 605 316 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2640 2655 2863 3998 4679 4870 826 Size: 219 1595 15 208 1135 681 191 1471 Fragments arranged by size: 1595 1471 1135 681 219 208 191 15 SfoI GGC'GCC Cuts at: 2161 2161 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2791 3619 4520 4782 820 Size: 197 926 848 828 901 262 1553 Fragments arranged by size: 1553 926 901 848 828 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2004 2724 2954 513 Size: 552 939 720 230 3074 Fragments arranged by size: 3074 939 720 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2734 3542 3559 3712 3897 4342 325 Size: 1255 1154 808 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1154 808 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 3761 5473 Size: 274 80 33 51 1443 729 1055 1712 Cuts at: 5473 96 Size: 138 Fragments arranged by size: 1712 1443 1055 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI